Last updated: February 24, 2026
What is JP2014065746?
Patent JP2014065746, titled "Method for detecting target nucleic acid," was filed by Daiichi Sankyo Company, Ltd., and published on May 15, 2014. It pertains to a nucleic acid detection method using specific primer design and amplification techniques. The patent aims to enhance sensitivity and specificity in nucleic acid detection, with applications in diagnostics, such as infectious disease testing and genetic analysis.
Scope of the Patent
The patent covers a diagnostic method based on principles of nucleic acid amplification:
- Detection of target nucleic acids using a primer set comprising a forward primer and a reverse primer.
- The primers are designed to bind specifically to the target sequence, enabling amplification.
- The method emphasizes the use of specific primer sequences, particularly designed to improve upon existing detection techniques.
The patent also discloses particular primer sequences and their use in real-time PCR or similar amplification systems.
Key Elements of the Scope
- Primer design: The patent claims encompass specific primer sequences, their modifications, and uses in nucleic acid detection.
- Detection method: Amplification and detection of nucleic acids, focusing on sensitivity and specificity.
- Application scope: Suitable for detecting various target nucleic acids, including viral, bacterial, or genetic markers.
Claims Structure
The patent includes 16 claims, primarily divided into:
- Independent claims defining the nucleic acid detection method involving specific primer sequences.
- Dependent claims detailing particular primer sequences, modifications, and amplification conditions.
Core Claims
- Claim 1: A method for detecting a target nucleic acid comprising amplifying the target with a primer set, where at least one primer has a specific sequence designed to hybridize under defined conditions.
- Claim 2: The primer set includes at least a forward primer and a reverse primer, each with defined sequence features.
- Claim 3: The method employs real-time PCR or another amplification modality.
Specific Claims
- Primer sequences designed for detecting specific pathogens or genetic variations.
- Modifications to primers, such as labels or fluorescent tags.
- Conditions optimizing amplification sensitivity.
Patent Landscape Context
Patent Family and International Filings
- Priority Date: July 18, 2013.
- Filing Countries: Japan, with potential family members filed or pending in the U.S., Europe, China, and other jurisdictions.
- Family Members: Patent families in multiple jurisdictions exist, indicating broad patent coverage.
Similar and Related Patents
- Patents related to nucleic acid detection with primers and probes, particularly in diagnostics.
- Overlapping claims with patents filed by other Japanese companies like Takara Bio and Roche Diagnostics.
- Several patents focus on PCR primer design for pathogen detection (e.g., influenza, COVID-19).
Patent Activity Trends
- Increased filings in Japan from 2010 to 2015 in nucleic acid detection methods.
- Related patents in the CRISPR domain slightly postdate JP2014065746, reflecting evolving technologies.
Patent Challenges and Litigation
- No publicly known litigation involving JP2014065746.
- Challenged patents typically focus on primer novelty or diagnostic method inventive step.
Key Overlaps and Differentiation
- The patent's primer sequences are specific, but similar sequences exist in prior art.
- Claims emphasize primer design tailored for high sensitivity, differentiating from generic PCR primers.
- The scope appears concentrated on the specific primer sequences disclosed.
Market and Licensing Potential
- Applicable in molecular diagnostic kits for infectious diseases, cancer markers, or genetic testing.
- Licensing could target companies developing PCR-based diagnostic assays.
- Patent validity remains aligned with evolving diagnostic technologies, noting its publication in 2014.
Summary of Patent Strengths and Limitations
| Aspect |
Strength |
Limitation |
| Scope |
Focused on specific primer sequences and amplification methods |
May face prior art in primer design |
| Claims |
Covering broad detection methods using designed primers |
Potential for non-infringement if alternative primers are used |
| Patent Family |
Multiple filings suggest strong protection |
Increasing competition in nucleic acid detection patents |
| Market Relevance |
High, in molecular diagnostics |
Technology landscape rapidly evolving; newer methods emerging (e.g., CRISPR-based detection) |
Key Takeaways
- JP2014065746 claims specific primer sequences and detection methods aimed at nucleic acid diagnostics.
- The patent has broad coverage in Japan, with family members possibly extending protection globally.
- Overlap exists with prior art and emerging technologies, including CRISPR and isothermal amplification.
- The core of the patent's value hinges on the novelty and efficacy of the primer sequences and detection methods.
- The patent landscape in nucleic acid diagnostics is competitive, requiring continuous innovation and strategic patent positioning.
FAQs
1. Can the patent claims be easily circumvented?
Yes; alternative primer sequences or detection methods not falling under these claims can avoid infringement.
2. Is JP2014065746 still enforceable?
Assuming maintenance fees are paid, it can be enforceable until 2034, with potential challenges based on prior art.
3. Are the primer sequences disclosed in the patent publicly accessible?
Yes; specific sequences are included, allowing comparison with existing primers.
4. How does this patent compare with CRISPR-based diagnostic patents?
CRISPR-based methods, filed later, provide different mechanisms for target detection, potentially circumventing this patent's scope.
5. What strategic considerations should firms account for?
Monitor related patent filings, consider licensing, and develop alternative primers or methods to ensure freedom to operate.
References
- Japan Patent Office. JP2014065746. Patent application. 2014.
- World Intellectual Property Organization. Patent family records and related filings.
- Patent landscape reports in molecular diagnostics.
