Claims for Patent: 7,598,089

Summary for Patent: 7,598,089

Title:	Methods and compositions for separating cells
Abstract:	The invention provides compositions and methods for cell separation. These reagents and techniques specifically agglutinate cells via surface antigen recognition and can be used to recover rare cell types in high yield.
Inventor(s):	Collins; Daniel P. (Lino Lakes, MN)
Assignee:	BioE, Inc. (St. Paul, MN)
Application Number:	11/406,790
Patent Claims:	1. A cell separation reagent comprising: a) dextran; b) anti-glycophorin A antibody; c) heparin; d) serum albumin; and e) ethylenediaminetetraacetic acid (EDTA) wherein dextran, anti-glycophorin A antibody, heparin, serum albumin, and EDTA are in solution. 2. The reagent of claim 1, further comprising phosphate buffered saline. 3. The reagent of claim 1, wherein the pH of said reagent is between 6.8 to 7.8. 4. The reagent of claim 1, wherein said serum albumin is bovine serum albumin. 5. The reagent of claim 1, wherein said serum albumin is human serum albumin. 6. The reagent of claim 1, wherein said anti-glycophorin A antibody is monoclonal. 7. The reagent of claim 1, wherein said anti-glycophorin A antibody is an IgM antibody or an IgG antibody. 8. The reagent of claim 1, wherein said anti-glycophorin A antibody is an anti-human glycophorin A antibody. 9. The reagent of claim 1, wherein the concentration of said anti-glycophorin A antibody is about 0.001 mg/L to about 15 mg/L. 10. The reagent of claim 1, wherein the concentration of said serum albumin is about 0.5% to about 5%. 11. The reagent of claim 1, wherein the concentration of EDTA is 0.1 mM to 5 mM. 12. A kit comprising a blood collection vessel and the cell separation reagent of claim 1. 13. The kit of claim 12, wherein said blood collection vessel is a blood bag. 14. The kit of claim 12, wherein said blood collection vessel is a vacuum tube. 15. A method for separating cells, said method comprising a) contacting a blood cell-containing sample with the cell separation reagent of claim 1; b) allowing said sample of step a) to partition into an agglutinate and a supernatant phase at 1 xg; and c) recovering said cells from the agglutinate or the supernatant phase. 16. The method of claim 15, wherein said sample is a human blood cell-containing sample. 17. The method of claim 15, wherein said sample is a peripheral blood sample. 18. The method of claim 15, wherein said sample is an umbilical cord sample. 19. The method of claim 15, wherein said sample is a bone marrow sample. 20. The method of claim 15, wherein said cells are recovered from said supernatant phase.

Details for Patent 7,598,089

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Grifols Therapeutics Llc	ALBUKED, PLASBUMIN-20, PLASBUMIN-25, PLASBUMIN-5	albumin (human)	For Injection	101138	10/21/1942		2040-01-28
Baxalta Us Inc.	BUMINATE, FLEXBUMIN	albumin (human)	Injection	101452	03/03/1954		2040-01-28
Csl Behring Ag	ALBURX	albumin (human)	Injection	102366	07/23/1976		2040-01-28
Grifols Biologicals Llc	ALBUTEIN	albumin (human)	Injection	102478	08/15/1978		2040-01-28
Instituto Grifols, S.a.	HUMAN ALBUMIN GRIFOLS	albumin (human)	Injection	103352	02/17/1995		2040-01-28