Claims for Patent: 5,342,771

Summary for Patent: 5,342,771

Title:	Functionalized imidazolinone haptens and protein conjugates thereof useful in assays for the detection of imidazolinone herbicides
Abstract:	This invention provides enzyme conjugates, antigens, antibodies and diagnostic test kits used in an enzyme-linked immunosorbent assay method for determining the presence and concentration of imidazolinone compounds.
Inventor(s):	Wong; Rosie B. (Piscataway, NJ), Ahmed; Zareen (Princeton Junction, NJ), Bullock; Milon W. (Skillman, NJ)
Assignee:	American Cyanamid Company (Wayne, NJ)
Application Number:	08/023,231
Patent Claims:	1. An antigen for producing an antibody against an imidazolinone having the structure ##STR16## wherein A is N or CH; R is H or CH.sub.3 ; X is H, halogen, methyl or hydroxyl; and Y and Z are each independently hydrogen, halogen, C.sub.1 -C.sub.4 alkyl, C.sub.1 -C.sub.4 alkoxy, C.sub.1 -C.sub.4 haloalkyl, nitro, cyano; or Y and Z are taken together to represent --CH.dbd.CH--CH.dbd.CH--; which comprises an imidazolinone hapten having the structural formula ##STR17## wherein W is ##STR18## E is --NH--N.dbd.CH-- or --NH--CH.sub.2 --; B is C.sub.2 alkyl or C.sub.2 alkenyl; n is an integer of 0, 1 or 2; Z is a protein; A is N or CH; and R is H or CH.sub.3. 2. The antigen according to claim 1 wherein the imidazolinone is selected from the group consisting of imazaquin, imazethapyr, imazamethabenz, imazapyr imazamethapyr and 2-(4-isopropyl)-4-methyl-5-oxo-2-imidazolin-2-yl)-5-(methoxymethyl)nicotin ic acid. 3. The antigen according to claim 1 wherein the protein is selected from the group consisting of bovine serum albumin, human serum albumin, fibrinogen, ovalbumin, thyroglobulin and keyhole limpet hemocyanin. 4. An antigen according to claim 1 having the formula ##STR19## wherein X is an integer from about 10 to 40 and BSA is bovine serum albumin. 5. An enzyme conjugate comprising an imidazolinone having the structural formula ##STR20## wherein A is N or CH; R is H or CH.sub.3 : R.sub.1 is NHQ, ##STR21## R.sub.2 is hydrogen; or R.sub.1 and R.sub.2 are taken together to represent ##STR22## ##STR23## m is an integer of 1 or 2; and wherein Q is an enzyme. 6. The enzyme conjugate according to claim 5 wherein the enzyme is selected from the group consisting of horseradish peroxidase, alkaline phosphatase and urease. 7. The enzyme conjugate according to claim 5 having the structural formula ##STR24## wherein HRP is horseradish peroxidase. 8. An imidazolinone hapten having the structural formula ##STR25## wherein W is S.dbd.C.dbd.N-- or ##STR26## B is C.sub.2 alkyl or C.sub.2 alkenyl; n is an integer of 0, 1 or 2 A is N or CH: and R is H or CH.sub.3 ; with the proviso that when A is N then n is not 0. 9. A hapten according to claim 8, 5-(2-formylvinyl)-2-(4-isopropyl-4-methyl-5-oxo-2-imidazolin-2-yl)benzoic acid. 10. A hapten according to claim 8, selected from the group consisting of methyl 6-(4-isopropyl-4-methyl-5-oxo-2-imidazolin-2-yl)isophthalaldehydate and 5-(4-formyl-1,3-butadienyl )-2-(4-isopropyl-4-methyl-5-oxo-2-imidazolin-2-yl) benzoic acid.

Details for Patent 5,342,771

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Grifols Therapeutics Llc	ALBUKED, PLASBUMIN-20, PLASBUMIN-25, PLASBUMIN-5	albumin (human)	For Injection	101138	10/21/1942		2011-08-30
Baxalta Us Inc.	BUMINATE, FLEXBUMIN	albumin (human)	Injection	101452	03/03/1954		2011-08-30
Csl Behring Ag	ALBURX	albumin (human)	Injection	102366	07/23/1976		2011-08-30