You’re using a public version of DrugPatentWatch with 5 free searches available | Register to unlock more free searches. CREATE FREE ACCOUNT

Last Updated: April 26, 2024

Claims for Patent: 5,028,524

✉ Email this page to a colleague

« Back to Dashboard

Summary for Patent: 5,028,524

Title:	Assay for anti-pre-S antibody
Abstract:	Assays for an anti-pre-S antibody in a human serum by an enzyme immunoassay in which a biotin-avidin system is used are disclosed. The assays include inhibition method, sandwich method, competitive method and Protein A or anti-human IgG antibody method in a solid phase. The assays can determine the anti-pre-S antibody titer in human sera highly sensitively and simply without use of radioactive materials.
Inventor(s):	Fujisawa; Yukio (Kobe, JP), Nishimura; Osamu (Kawanishi, JP), Itoh; Yasuaki (Tsukuba, JP)
Assignee:	Takeda Chemical Industries, Ltd. (Osaka, JP)
Application Number:	07/181,210
Patent Claims:	1. An assay for an anti-pre-S2 antibody in a human serum specimen which comprises reacting a human serum specimen, a biotinated pre-S2 peptide having the amino acid sequence: and an avidinated enzyme with an anti-pre-S2 antibody which is bound to a carrier, and measuring enzyme activity on the carrier wherein the amount of measured enzyme activity is inversely proportional to the amount of pre S-2 antibody in the specimen. 2. The assay as claimed in claim 1, wherein the enzyme is horseradish peroxidase. 3. The assay as claimed in claim 1, wherein the carrier is a particle or a plate. 4. An assay for an anti-pre-S2 antibody in a human serum specimen which comprises reacting a human serum specimen with a pre-S2 peptide having the amino acid sequence: which is bound to a carrier, then adding a biotinated pre-S2 peptide having the amino acid sequence: and an avidinated enzyme thereto, and measuring enzyme activity on the carrier wherein the amount of measured enzyme activity is proportional to the amount of pre S-2 antibody in the specimen. 5. The assay as claimed in claim 4, wherein the enzyme is horseradish peroxidase. 6. The assay as claimed in claim 4, wherein the carrier is a particle or a plate. 7. An assay for an anti-pre-S2 antibody in a human serum specimen which comprises reacting a human serum specimen, a biotinated anti-pre-S2 antibody and an avidinated enzyme with a pre-S2 peptide having the amino acid sequence: which is bound to a carrier, and measuring enzyme activity on the carrier wherein the amount of measured enzyme activity is inversely proportional to the amount of pre S-2 antibody in the specimen. 8. The assay as claimed in claim 7, wherein the enzyme is horseradish peroxidase. 9. The assay as claimed in claim 7, wherein the carrier is a particle or a plate. 10. An assay for an anti-pre-S2 antibody in a human serum specimen which comprises reacting a human serum specimen, a biotinated pre-S2 peptide having the amino acid sequence: and an avidinated enzyme with an anti-human immunoglobulin G antibody or Protein A which is bound to a carrier, and measuring enzyme activity on the carrier wherein the amount of measured enzyme activity is proportional to the amount of pre S-2 antibody in the specimen. 11. The assay as claimed in claim 10, wherein the enzyme is horseradish peroxidase. 12. The assay as claimed in claim 10, wherein the carrier is a particle or a plate.

Details for Patent 5,028,524

Subscribe to access the full database, or Try a Trial
Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Csl Behring Ag	CARIMUNE, CARIMUNE NF, PANGLOBULIN, SANDOGLOBULIN	immune globulin intravenous (human)	For Injection	102367	07/27/2000	⤷ Try a Trial	2008-07-02
Csl Behring Ag	PRIVIGEN	immune globulin intravenous (human), 10% liquid	Injection	125201	07/26/2007	⤷ Try a Trial	2008-07-02
Csl Behring Ag	PRIVIGEN	immune globulin intravenous (human), 10% liquid	Injection	125201	10/02/2009	⤷ Try a Trial	2008-07-02
Csl Behring Ag	PRIVIGEN	immune globulin intravenous (human), 10% liquid	Injection	125201	02/07/2013	⤷ Try a Trial	2008-07-02
Bio Products Laboratory	GAMMAPLEX	immune globulin intravenous (human)	Injection	125329	09/17/2009	⤷ Try a Trial	2008-07-02
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

Make Better Decisions: Try a trial or see plans & pricing

Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.