Claims for Patent: 4,835,258

Summary for Patent: 4,835,258

Title:	Conjugation of aromatic amines or nitro-containing compounds with proteins or polypeptides by photoirradiation of the azide derivatives with ultraviolet light in order to produce antibodies against the haptens
Abstract:	A method of linking primary aromatic amine- or nitro-compounds to carrier proteins by photochemical reactions in order to produce antibodies against the haptens.
Inventor(s):	Hollenberg; Paul F. (Skokie, IL), Pandey; Ramendra N. (Lombard, IL)
Assignee:	Northwestsern University (Evanston, IL)
Application Number:	06/946,436
Patent Claims:	1. A method of conjugating nitro or amino containing compounds to carrier proteins, without loss of function of the carrier proteins, to elicit an antibody response against the hapten wherein the improvement comprises irradiating the protein in the presence of the azido derivatives of the primary aromatic amino- or nitro-compound at physiological pH. 2. The method of claim 1 wherein the amount of time needed for the conjugation of the azido derivatives of the nitro- or amino-containing compounds to the carrier proteins is about 1 second to about 30 minutes. 3. The method of claim 1 wherein the protein conjugated can be isolated in about 2 to about 3 hours. 4. The method of claim 1 wherein the pH ranges from about 6.8 to about 7.8. 5. The method of claim 1 wherein the carrier proteins may be any protein or polypeptide. 6. The method of claim 1 wherein the carrier protein may be isolated from human or animal serum, tissue, cellular extracts or protein components of pathogenic organisms. 7. The method of claim 5 wherein the carrier protein may be selected from the group of proteins and polypeptides selected from the group consisting of bovine serum albumin, human transferrin, thyroglobulin, poly (lysine.tyrosine) and poly (lysine.phenylalanine). 8. The method of claim 1 wherein the nitro or amino containing compound may be a primary aromatic amine. 9. The method of claim 8 wherein the primary aromatic amine is converted to an azide compound. 10. The method of claim 9 wherein the azide compound is selected from the group consisting of 3-azido-N-ethyl carbazole, 1-azidopyrene, 2-azidoflourene, 2-azidonapthalene. 11. The method of claim 1 wherein the structure of the linkage region between the hapten and carrier protein is R--NH-carrier, wherein R is an aryl residue. 12. The method of claim 1 wherein the irradiation is accomplished by irradiation with ultravioloet or visible light. 13. The method of claim 1 wherein the duration of the irradiation varies from about 1 second to about 30 minutes.

Details for Patent 4,835,258

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Grifols Therapeutics Llc	ALBUKED, PLASBUMIN-20, PLASBUMIN-25, PLASBUMIN-5	albumin (human)	For Injection	101138	10/21/1942		2040-01-28
Baxalta Us Inc.	BUMINATE, FLEXBUMIN	albumin (human)	Injection	101452	03/03/1954		2040-01-28
Csl Behring Ag	ALBURX	albumin (human)	Injection	102366	07/23/1976		2040-01-28