Claims for Patent: 4,461,832

Summary for Patent: 4,461,832

Title:	Preparation of an enzymatically active formulation embedded in silica gel
Abstract:	A process for the preparation of an enzymatically active formulation embedded in silica gel, wherein an aqueous mixture of an enzymatically active formulation and a dissolved alkali metal silicate and/or ammonium silicate is suspended in an organic, water-immiscible fluid and then converted to a water-insoluble gel.
Inventor(s):	Tschang; Chung-Ji (Frankenthal, DE), Klefenz; Heinrich (Hochdorf-Assenheim, DE), Sanner; Axel (Frankenthal, DE), Zahn; Wolfgang (Altrip, DE)
Assignee:	BASF Aktiengesellschaft (DE)
Application Number:	06/376,597
Patent Claims:	1. A process for the preparation of an enzymatically active formulation embedded in silica gel which comprises: suspending an aqueous mixture consisting of an enzymatically active formulation and a dissolved alkali metal silicate and/or ammonium silicate as droplets in a stirred organic, water-immiscible fluid and then converting said silicate to a water-insoluble gel. 2. A process as claimed in claim 1, wherein the enzymatically active formulation used consists of active cells or cell fragments of microbiological, vegetable, animal or human origin. 3. A process set forth in claim 1, wherein the formation of a suspension is assisted by using a suspending agent. 4. A process as set forth in claim 1, 2 or 3, wherein the gelling is effected by lowering the pH by means of an agent which is soluble in water and in the organic water-immiscible fluid. 5. A process of claim 4 wherein said agent is an organic acid. 6. A process as set forth in claim 1, wherein the gelling is effected in the presence of an inert material. 7. A process as claimed in claim 1, wherein said enzymatically active formulation embody as micro-organisms one of Streptomyces, Arthrobacter or Bacillus microorganisms, or Escherichia coli, Saccharomuces, Curvularia lunata, or Aspergillus ochraceus. 8. A process as claimed in claim 1, wherein the enzymatically active formulation embody of one of trypsin, chymotrypsin, pancreatin, .alpha.- and .beta.-amylase, ribonucleases, desoxyribonucleases, cellulase, maltase, pectinase, chitinase, pepsin, bromelain, keratinase, amyloglycosidase, lipase, cholinesterase, lecithinase, phosphatase, alginase, asparaginase, glutaminase, urease, lactase, penicillin-amidase, penicillinase, glucose-isomerase, glucose-oxidase, catalase, peroxidase, lipoxidase, xanthin-oxidase, cytochrome-reductase, lactic acid oxidase, aminoacid oxidase, rennin, ficin, subtilisin, tannase, phenol-oxidase, pullulanase, isoamylase, hexokinase, galactose-oxidase, diaphorase, aldolase, glycollic acid oxidase, luciferase, aldehyde-oxidase, naringinase, uricase, glutathione-reductase, nitrito-reductase, nitrate-reductase, succinic acid dehydrogenase, catechol-oxidase, .beta.-fructosidase, aminoacid acylase and urokinase. 9. A process as claimed in claim 1, wherein said enzymatically active formulation comprises baker's yeast. 10. A process as claimed in claim 1, wherein the embedded enzymatic formulation is immobilized by the embedding thereof in said gel. 11. A process for the preparation of an enzymatically active formulation embedded in silica gel which comprises: suspending an aqueous mixture consisting of an enzymatically active formulation, soluble sulfates or phosphates and a dissolved alkali metal silicate and/or ammonium silicate as droplets in a stirred organic, water-immiscible fluid, then converting said silicate to a water-insoluble gel, and thereafter treating the product obtained with a solution of salt of which the cation forms a sparingly soluble sulfate or phosphate.

Details for Patent 4,461,832

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Microbix Biosystems Inc.	KINLYTIC	urokinase	For Injection	021846	01/16/1978		2001-07-24
Recordati Rare Diseases, Inc.	ELSPAR	asparaginase	For Injection	101063	01/10/1978		2001-07-24