Claims for Patent: 4,438,198

Summary for Patent: 4,438,198

Title:	Biochemically active matrix for use in a bio-artificial organ
Abstract:	A biochemically active matrix for use in a bio-artificial organ is disclosed. The biochemically active matrix has an enzyme covalently bonded to a matrix of organochemically cross-linked fibrin. The matrix may be suspended in a medium of agarose which irreversibly solidifies below 37.degree. C. The bio-artificial organ is useful for extracorporeal treatment of blood to remove excess substrate from the blood. .
Inventor(s):	Schmer; Gottfried (Seattle, WA)
Assignee:	Trimedyne, Inc. (Arlington Heights, IL)
Application Number:	06/307,073
Patent Claims:	1. A biochemically active matrix suitable for use in a bio-artificial organ comprising at least one biochemically active enzyme covalently bonded to the surface of a carrier matrix of organochemically cross-linked fibrin through a reactive group of the enzyme which is not essential for its enzymatic activity and suspended in a medium of agarose which irreversibly solidifies below 37.degree. C. 2. The biochemically active matrix of claim 1 wherein the biochemically active enzyme is covalently bonded to the surface of the matrix by glutaraldehyde. 3. The biochemically active matrix of claim 1 wherein the biochemically active enzyme is L-asparaginase. 4. The biochemically active matrix of claim 1 wherein the biochemically active enzyme is urease. 5. The biochemically active matrix of claim 1 wherein the biochemically active enzyme is indolyl-3-alkane-.alpha.-hydroxylase. 6. The biochemically active matrix of claim 1 wherein said enzyme is asparaginase and is covalently bonded by glutaraldehyde. 7. A bio-artificial organ comprising: (a) a reactor housing defining an enclosed space with an inlet and an outlet, the inner surface of the housing being compatible with an extracorporeal blood flow; (b) a carrier matrix of organochemically cross-linked fibrin disposed within the reactor, said carrier matrix being suspended in a medium of agarose which gels irreversibly below 37.degree. C.; and (c) a biochemically active enzyme covalently bonded to the surface of the matrix through a reactive group of the enzyme which is not essential for its enzymatic activity. 8. The bio-artificial organ of claim 7 wherein the biochemically active enzyme is covalently bonded to the surface of the matrix by glutaraldehyde. 9. The bio-artificial organ of claim 7 the biochemically active enzyme is L-asparaginase. 10. The bio-artificial organ of claim 7 wherein the carrier matrix is disposed on a physical support means.

Details for Patent 4,438,198

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Recordati Rare Diseases, Inc.	ELSPAR	asparaginase	For Injection	101063	01/10/1978		2039-03-29