Claims for Patent: 10,526,382

Summary for Patent: 10,526,382

Title:	Human neutrophil gelatinase-associated lipocalin (hNGAL) muteins capable of binding angiopoietin-2 (Ang-2) and methods of use thereof
Abstract:	The present disclosure provides hNGAL muteins that bind Ang-2 and can be used in various application including pharmaceutical applications, for example, to inhibit or reduce angiogenesis. The present disclosure also concerns methods of making one or more muteins described herein as well as compositions and combinations comprising one or more of such muteins. The present disclosure further relates to nucleic acid molecules encoding such muteins and to methods for generation of such muteins and nucleic acid molecules. In addition, the application discloses therapeutic and/or diagnostic uses of these muteins as well as compositions and combinations comprising one or more of such muteins.
Inventor(s):	Bel Aiba; Rachida SIham (Munich, DE), Allersdorfer; Andrea (Wolnzach, DE), Wiedenmann; Alexander (Ulm, DE), Rothe; Christine (Dachau, DE), Olwill; Shane (Freising, DE), Gille; Hendrik (Berlin, DE), Audoly; Laurent (Toulouse, FR)
Assignee:	Pieris Pharmaceuticals GmbH (Freising-Weihenstephan, DE)
Application Number:	15/546,609
Patent Claims:	1. A human neutrophil gelatinase associated lipocalin (hNGAL) mutein that is capable of binding angiopoietin-2 (Ang-2) with detectable affinity, wherein the mutein comprises the following mutated amino acid residue in comparison with the linear polypeptide sequence of mature hNGAL (SEQ ID NO: 16): Gln 28.fwdarw.His, wherein the mutein comprises at least fifteen of the following mutated amino acid residues in comparison with the linear polypeptide sequence of mature hNGAL (SEQ ID NO: 16): Leu 36.fwdarw.Gln, Glu, His, Val, Met or Phe; Ala 40.fwdarw.Val, Tyr, His or Trp; Ile 41.fwdarw.His, Tyr, Trp, Val, Arg, Glu, or Asp; Gln 49.fwdarw.Gly, Ile, Glu or Val; Tyr 52.fwdarw.Trp, His, Thr or Ser; Ser 68.fwdarw.Gly, Asp, Gln, Glu or Ile; Leu 70.fwdarw.Ser, Thr, Gly, Arg, Tyr or Ala; Arg 72 Gly, Ala, Trp, Thr or Glu; Lys 73.fwdarw.Pro, Phe, Leu, Arg, Ala or Gln; Asp 77.fwdarw.Asn, Lys, Ser or Val; Trp 79.fwdarw.Thr, Arg, Ser or Asn; Arg 81.fwdarw.Trp, His or Tyr; Asn 96.fwdarw.Gly, Ala, Pro, Gln or Asp; Tyr 100.fwdarw.Pro, Trp, Gly, Ser, Leu or Asp; Leu 103.fwdarw.Gly, Glu, Asp, Met or Gln; Tyr 106.fwdarw.Thr, Leu, Phe, or Pro; Lys 125.fwdarw.His, Thr or Gly; Ser 127.fwdarw.Leu, Met, or Tyr; Tyr 132.fwdarw.Phe, Trp or Val; and Lys 134.fwdarw.Ala, Glu or Trp, and wherein the mutein has a sequence identity of at least 85% to a sequence selected from the group consisting of SEQ ID NOs: 1-14. 2. The hNGAL mutein of claim 1, wherein the mutein is capable of binding Ang-2 with a dissociation constant (KD) of about 5 nM or lower. 3. The hNGAL mutein of claim 1, wherein the mutein is capable of binding Ang-2 with an affinity measured by an EC50 value of about 5 nM or lower. 4. The hNGAL mutein of claim 1, wherein the mutein is capable of binding Ang-2 with an affinity measured by an IC50 value of about 5 nM or lower. 5. The hNGAL mutein of claim 1, wherein the mutein is capable of inhibiting or reducing lymphatic microvascular endothelial cell proliferation mediated by Ang-2 with an IC50 value of about 5 nM or lower. 6. The hNGAL mutein of claim 1, wherein the mutein comprises a mutated amino acid residue at sixteen or more positions corresponding to positions 36, 40, 41, 49, 52, 65, 68, 70, 72-74, 77, 79, 81, 87, 96, 100, 103, 106, 116, 125, 126, 127, 129, 132, and 134 of the linear polypeptide sequence of mature hNGAL (SEQ ID NO: 16). 7. The hNGAL mutein of claim 1, wherein the mutein comprises one or more of the following mutated amino acid residues in comparison with the linear polypeptide sequence of mature hNGAL (SEQ ID NO: 16): Asn 65.fwdarw.Asp; Lys 74.fwdarw.Glu; Cys 87.fwdarw.Ser; Asn 116.fwdarw.Asp; Val 126.fwdarw.Met; and Asn 129.fwdarw.Asp. 8. The hNGAL mutein of claim 1, wherein the mutein comprises one of the following sets of mutated amino acid residues in comparison with the linear polypeptide sequence of mature hNGAL (SEQ ID NO: 16): (a) Gln 28.fwdarw.His; Leu 36.fwdarw.Gln; Ala 40.fwdarw.Tyr; Gln 49.fwdarw.Gly; Tyr 52.fwdarw.Trp; Ser 68.fwdarw.Gly; Leu 70.fwdarw.Ser; Arg 72.fwdarw.Gly; Lys 73.fwdarw.Pro; Asp 77.fwdarw.Asn; Trp 79.fwdarw.Thr; Arg 81.fwdarw.Trp; Cys 87.fwdarw.Ser; Asn 96.fwdarw.Gly; Tyr 100.fwdarw.Pro; Leu 103.fwdarw.Gly; Tyr 106.fwdarw.Thr; Lys 125.fwdarw.His; Ser 127.fwdarw.Leu; Tyr 132.fwdarw.Phe; Lys 134.fwdarw.Glu; (b) Gln 28.fwdarw.His; Leu 36.fwdarw.Phe; Ala 40.fwdarw.His; Ile 41.fwdarw.Arg; Gln 49.fwdarw.Gly; Tyr 52.fwdarw.His; Ser 68.fwdarw.Asp; Leu 70.fwdarw.Thr; Arg 72.fwdarw.Ala; Lys 73.fwdarw.Phe; Asp 77.fwdarw.Asn; Trp 79.fwdarw.Arg; Arg 81.fwdarw.His; Cys 87.fwdarw.Ser; Tyr 100.fwdarw.Trp; Leu 103.fwdarw.Glu; Tyr 106.fwdarw.Thr; Lys 125.fwdarw.Thr; Ser 127.fwdarw.Met; Tyr 132.fwdarw.Trp; Lys 134.fwdarw.Trp; (c) Gln 28.fwdarw.His; Leu 36.fwdarw.Val; Ala 40.fwdarw.Trp; Ile 41.fwdarw.Tyr; Gln 49.fwdarw.Ile; Tyr 52.fwdarw.Thr; Ser 68.fwdarw.Gln; Leu 70.fwdarw.Gly; Arg 72.fwdarw.Glu; Lys 73.fwdarw.Gln; Asp 77.fwdarw.Lys; Trp 79.fwdarw.Ser; Arg 81.fwdarw.His; Cys 87.fwdarw.Ser; Asn 96.fwdarw.Asp; Tyr 100.fwdarw.Trp; Leu 103.fwdarw.Asp; Tyr 106.fwdarw.Leu; Lys 125.fwdarw.Gly; Ser 127.fwdarw.Met; Tyr 132.fwdarw.Val; Lys 134.fwdarw.Ala; (d) Gln 28.fwdarw.His; Leu 36.fwdarw.Glu, Ala 40.fwdarw.Val; Ile 41.fwdarw.Glu; Gln 49.fwdarw.Val; Tyr 52.fwdarw.Thr Ser 68.fwdarw.Glu; Leu 70.fwdarw.Arg; Arg 72.fwdarw.Trp; Lys 73.fwdarw.Leu; Asp 77.fwdarw.Lys; Trp 79.fwdarw.Asn; Arg 81.fwdarw.His; Cys 87.fwdarw.Ser; Asn 96.fwdarw.Ala; Tyr 100.fwdarw.Gly; Leu 103.fwdarw.Met; Tyr 106.fwdarw.Thr; Lys 125.fwdarw.Thr; Ser 127.fwdarw.Met; Tyr 132.fwdarw.Trp; Lys 134.fwdarw.Trp; (e) Gln 28.fwdarw.His; Leu 36.fwdarw.Gln; Ala 40.fwdarw.Tyr; Ile 41.fwdarw.Trp; Gln 49.fwdarw.Ile; Tyr 52.fwdarw.Ser; Ser 68.fwdarw.Ile; Leu 70.fwdarw.Tyr; Arg 72.fwdarw.Thr; Lys 73.fwdarw.Arg; Asp 77.fwdarw.Ser; Trp 79.fwdarw.Arg; Arg 81.fwdarw.Tyr; Cys 87.fwdarw.Ser; Asn 96.fwdarw.Pro; Leu 103.fwdarw.Asp; Tyr 106.fwdarw.Thr; Lys 125.fwdarw.His; Ser 127.fwdarw.Tyr; Tyr 132.fwdarw.Trp; Lys 134.fwdarw.Glu; (f) Gln 28.fwdarw.His; Leu 36.fwdarw.Gln; Ala 40.fwdarw.Tyr; Gln 49.fwdarw.Glu; Tyr 52.fwdarw.Trp; Asn 65.fwdarw.Asp; Ser 68.fwdarw.Gly; Leu 70.fwdarw.Ser; Arg 72.fwdarw.Gly; Lys 73.fwdarw.Pro; Asp 77.fwdarw.Asn; Trp 79.fwdarw.Arg; Arg 81.fwdarw.Trp; Cys 87.fwdarw.Ser; Asn 96.fwdarw.Gly; Tyr 100.fwdarw.Ser; Leu 103.fwdarw.Gln; Tyr 106.fwdarw.Thr; Lys 125.fwdarw.His; Ser 127.fwdarw.Leu; Tyr 132.fwdarw.Phe; Lys 134.fwdarw.Glu; (g) Gln 28.fwdarw.His; Leu 36.fwdarw.His; Ala 40.fwdarw.Tyr; Gln 49.fwdarw.Glu; Tyr 52.fwdarw.Trp; Asn 65.fwdarw.Asp; Ser 68.fwdarw.Glu; Leu 70.fwdarw.Ser; Arg 72.fwdarw.Gly; Lys 73.fwdarw.Pro; Asp 77.fwdarw.Asn; Trp 79.fwdarw.Arg; Arg 81.fwdarw.Trp; Cys 87.fwdarw.Ser; Asn 96.fwdarw.Gly; Tyr 100.fwdarw.Pro; Leu 103.fwdarw.Asp; Tyr 106.fwdarw.Thr; Lys 125.fwdarw.His; Ser 127.fwdarw.Leu; Tyr 132.fwdarw.Phe; Lys 134.fwdarw.Glu; (h) Gln 28.fwdarw.His; Leu 36.fwdarw.Gln; Ala 40.fwdarw.Tyr; Gln 49.fwdarw.Gly; Tyr 52 Trp; Asn 65.fwdarw.Asp; Ser 68.fwdarw.Glu; Leu 70.fwdarw.Ser; Arg 72.fwdarw.Gly; Lys 73.fwdarw.Ala; Asp 77.fwdarw.Asn; Trp 79.fwdarw.Arg; Arg 81.fwdarw.Trp; Cys 87.fwdarw.Ser; Asn 96.fwdarw.Gly; Tyr 100.fwdarw.Asp; Leu 103.fwdarw.Gly; Tyr 106.fwdarw.Thr; Lys 125.fwdarw.His; Ser 127.fwdarw.Leu; Tyr 132.fwdarw.Phe; Lys 134.fwdarw.Glu; (i) Gln 28.fwdarw.His; Leu 36.fwdarw.His; Ala 40.fwdarw.Tyr; Gln 49.fwdarw.Gly; Tyr 52 Trp; Asn 65.fwdarw.Asp; Ser 68.fwdarw.Glu; Leu 70.fwdarw.Ser; Arg 72.fwdarw.Gly; Lys 73.fwdarw.Pro; Asp 77.fwdarw.Asn; Trp 79.fwdarw.Arg; Arg 81.fwdarw.Trp; Cys 87.fwdarw.Ser; Asn 96.fwdarw.Gly; Tyr 100.fwdarw.Pro; Leu 103.fwdarw.Gly; Tyr 106.fwdarw.Thr; Lys 125.fwdarw.His; Ser 127.fwdarw.Leu; Tyr 132.fwdarw.Phe; Lys 134.fwdarw.Glu; (j) Gln 28.fwdarw.His; Leu 36.fwdarw.Gln; Ala 40.fwdarw.Tyr; Tyr 52 Trp; Asn 65.fwdarw.Asp; Ser 68.fwdarw.Gly; Leu 70.fwdarw.Ser; Arg 72.fwdarw.Gly; Lys 73.fwdarw.Ala; Asp 77.fwdarw.Val; Trp 79.fwdarw.Arg; Arg 81.fwdarw.Trp; Cys 87.fwdarw.Ser; Asn 96.fwdarw.Gly; Tyr 100.fwdarw.Pro; Leu 103.fwdarw.Gly; Tyr 106.fwdarw.Thr; Lys 125.fwdarw.His; Ser 127.fwdarw.Leu; Tyr 132.fwdarw.Phe; Lys 134.fwdarw.Glu; (k) Gln 28.fwdarw.His; Leu 36.fwdarw.Gln; Ala 40.fwdarw.Tyr; Gln 49.fwdarw.Val; Tyr 52 Trp; Asn 65.fwdarw.Asp; Ser 68.fwdarw.Glu; Leu 70.fwdarw.Ser; Arg 72.fwdarw.Gly; Lys 73.fwdarw.Pro; Asp 77.fwdarw.Asn; Trp 79.fwdarw.Arg; Arg 81.fwdarw.Trp; Cys 87.fwdarw.Ser; Asn 96.fwdarw.Gly; Tyr 100.fwdarw.Leu; Leu 103.fwdarw.Gly; Tyr 106.fwdarw.Thr; Lys 125.fwdarw.His; Ser 127.fwdarw.Leu; Tyr 132.fwdarw.Phe; Lys 134.fwdarw.Glu; (l) Gln 28.fwdarw.His; Leu 36.fwdarw.Val; Ala 40.fwdarw.Trp; Ile 41.fwdarw.Tyr; Gln 49.fwdarw.Ile; Tyr 52 Thr; Asn 65.fwdarw.Asp; Ser 68.fwdarw.Gln; Leu 70.fwdarw.Gly; Arg 72.fwdarw.Glu; Lys 73.fwdarw.Gln; Lys 74.fwdarw.Glu; Asp 77.fwdarw.Lys; Trp 79.fwdarw.Ser; Arg 81.fwdarw.His; Cys 87.fwdarw.Ser; Tyr 100.fwdarw.Trp; Leu 103.fwdarw.Asp; Tyr 106.fwdarw.Pro; Asn 116.fwdarw.Asp; Lys 125.fwdarw.Gly; Ser 127.fwdarw.Met; Asn 129.fwdarw.Asp; Tyr 132.fwdarw.Val; Lys 134.fwdarw.Ala; (m) Gln 28.fwdarw.His; Leu 36.fwdarw.Val; Ala 40.fwdarw.Trp; Ile 41.fwdarw.Tyr; Gln 49.fwdarw.Ile; Tyr 52 Thr; Asn 65.fwdarw.Asp; Ser 68.fwdarw.Gln; Leu 70.fwdarw.Gly; Arg 72.fwdarw.Glu; Lys 73.fwdarw.Gln; Lys 74.fwdarw.Glu; Asp 77.fwdarw.Lys; Trp 79.fwdarw.Ser; Arg 81.fwdarw.His; Cys 87.fwdarw.Ser; Asn 96.fwdarw.Asp; Tyr 100.fwdarw.Trp; Leu 103.fwdarw.Asp; Tyr 106.fwdarw.Pro; Lys 125.fwdarw.Gly; Val 126.fwdarw.Met; Ser 127.fwdarw.Met; Asn 129.fwdarw.Asp; Tyr 132.fwdarw.Val; Lys 134.fwdarw.Ala; or (n) Gln 28.fwdarw.His; Leu 36.fwdarw.Met; Ala 40.fwdarw.Trp; Ile 41.fwdarw.Asp; Gln 49.fwdarw.Ile; Tyr 52 Thr; Asn 65.fwdarw.Asp; Ser 68.fwdarw.Gln; Leu 70.fwdarw.Ala; Arg 72.fwdarw.Glu; Lys 73.fwdarw.Gln; Asp 77.fwdarw.Lys; Trp 79.fwdarw.Ser; Arg 81.fwdarw.His; Cys 87.fwdarw.Ser; Asn 96.fwdarw.Gln; Tyr 100.fwdarw.Trp; Leu 103.fwdarw.Asp; Tyr 106.fwdarw.Pro; Lys 125.fwdarw.Gly; Ser 127.fwdarw.Met; Tyr 132.fwdarw.Val; Lys 134.fwdarw.Ala. 9. The hNGAL mutein of claim 1, wherein the mutein comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 1-14. 10. The hNGAL mutein of claim 1, wherein the mutein has at least 90% identity to an amino acid sequence selected from the group consisting of SEQ ID NOs: 1-14. 11. The hNGAL mutein of claim 1, wherein the mutein is capable of binding angiopoietin-1 (Ang-1) with detectable affinity. 12. The hNGAL mutein of claim 11, wherein the mutein is capable of binding Ang-1 with an affinity measured by an IC50 value of about 150 nM or lower. 13. The hNGAL mutein of claim 11, wherein the mutein comprises a mutated amino acid residue at sixteen or more positions corresponding to positions 36, 40, 41, 49, 52, 65, 68, 70, 72-74, 77, 79, 81, 87, 96, 100, 103, 106, 116, 125, 126, 127, 129, 132, and 134 of the linear polypeptide sequence of mature hNGAL (SEQ ID NO: 16). 14. The hNGAL mutein of claim 11, wherein the mutein comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 1-14. 15. The hNGAL mutein of claim 11, wherein said mutein has at least 90% identity to an amino acid sequence selected from the group consisting of SEQ ID NOs: 1-14. 16. The hNGAL mutein of claim 1, wherein the mutein is cross-reactive with both human Ang-2 and mouse Ang-2. 17. The hNGAL mutein of claim 16, wherein the mutein is capable of binding mouse Ang-2 with an affinity measured by an IC50 value of about 5 nM or lower. 18. The hNGAL mutein of claim 1, wherein the mutein is capable of blocking the binding of human Ang-2 to human Tie-2 with an IC50 value of about 25 nM or lower. 19. The hNGAL mutein of claim 1, wherein the mutein comprises one or more non-native cysteine residues substituting one or more amino acids of mature hNGAL (SEQ ID NO: 16). 20. The hNGAL mutein of claim 19, wherein the mutein comprises at least one amino acid substitution of a native cysteine residue by another amino acid. 21. The hNGAL mutein of claim 20, wherein said another amino acid is a serine residue. 22. The hNGAL mutein of claim 1, wherein the mutein is conjugated to a compound selected from the group consisting of an organic molecule, an enzyme label, a radioactive label, a colored label, a fluorescent label, a chromogenic label, a luminescent label, a hapten, digoxigenin, biotin, a cytostatic agent, a toxin, a metal complex, a metal, and colloidal gold. 23. The hNGAL mutein of claim 1, wherein the mutein is fused at its N-terminus and/or its C-terminus, with or without an intervening linker, to a fusion partner. 24. The hNGAL mutein of claim 23, wherein the fusion partner is selected from the group consisting of an antibody, an antibody fragment, a protein, a protein domain, a peptide, or a lipocalin mutein. 25. The hNGAL mutein of claim 1, wherein the mutein is conjugated to a compound that extends the serum half-life of the mutein. 26. The hNGAL mutein of claim 25, wherein the compound that extends the serum half-life is selected from the group consisting of a polyalkylene glycol molecule, hydroxyethyl starch, an Fc part of an immunoglobulin, a CH3 domain of an immunoglobulin, a CH4 domain of an immunoglobulin, an albumin binding peptide, and an albumin binding protein. 27. The hNGAL mutein of claim 26, wherein the polyalkylene glycol is polyethylene glycol (PEG) or an activated derivative thereof. 28. A pharmaceutical composition comprising an hNGAL mutein of claim 1. 29. The composition of claim 28, wherein the composition further includes at least one pharmaceutically acceptable adjuvant, diluent or carrier. 30. A composition comprising at least (a) an hNGAL mutein of claim 1 and (b) an anti-angiogenic agent. 31. The composition of claim 30, wherein the mutein and the anti-angiogenic agent are administered in combination, including concurrently, concomitantly or in series. 32. The composition of claim 30, wherein the mutein and the anti-angiogenic agent are administered independent from each other, including at individual intervals at independent points of time. 33. The composition of claim 30, wherein the anti-angiogenic agent is selected from the group consisting of: (i) antagonists of angiopoietin (Ang)-1, Ang-2, Ang-3, Ang-4, and/or Tie-2; (ii) antagonists of fms-like tyrosine kinase receptor 1 (Flt1), kinase insert domain-containing receptor (KDR), fms-like tyrosine kinase 4 (Flt4), vascular endothelial growth factor (VEGF)-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, placental growth factor (PIGF), and/or endocrine gland-derived VEGF (EG-VEGF); (iii) delta-like ligand 4 (DLL4, a vascular-specific Notch ligand) antagonists, (iv) epidermal growth factor receptor (EGFR) antagonists, and (v) cytokine inhibitors. 34. The composition of claim 30, wherein the anti-angiogenic agent is a VEGF inhibitor. 35. The composition of claim 34, wherein the VEGF inhibitor is selected from the group consisting of: VEGF-Trap, bevacizumab, sorafenib, sunitinib, and pazopanib. 36. The composition of claim 34, wherein the VEGF inhibitor is an antagonist of VEGF-A and/or an antagonist of VEGF-C. 37. The composition of claim 30, wherein the composition further comprises an additional anti-angiogenic agent. 38. The composition of claim 37, wherein the first anti-angiogenic agent is a VEGF-A antagonist and the second anti-angiogenic agent is a VEGF-C antagonist. 39. The composition of claim 38, wherein the antagonist of VEGF-A is an anti-VEGF-A antibody or a lipocalin mutein that has binding specificity for VEGF-A. 40. The composition of claim 38, wherein the antagonist of VEGF-C is an anti-VEGF-C antibody or a lipocalin mutein that has binding specificity for VEGF-C. 41. A diagnostic or analytical kit comprising an hNGAL mutein of claim 1. 42. A method of detecting the presence of Ang-2 in a sample, comprising the steps of (a) contacting the sample with an hNGAL mutein of claim 1 under conditions that allow the formation of a complex between the mutein and Ang-2, and (b) detecting the complex for the presence of Ang-2. 43. A method for the separation of Ang-2 in a sample, comprising the steps of (a) contacting the sample with an hNGAL mutein of claim 1 under conditions that allow the formation of a complex between the mutein and Ang-2, and (b) separating the complex from the sample. 44. A method of binding Ang-2 in a subject, comprising administering to said subject an hNGAL mutein of claim 1 or a composition comprising such mutein. 45. A method of inhibiting the binding of Ang-2 to Tie-2 in a subject, comprising administering to said subject an hNGAL mutein of claim 1 or a composition comprising such mutein. 46. A method of inhibiting or reducing angiogenesis in a subject, comprising administering to said subject an effective amount of an hNGAL mutein of claim 1 or a composition comprising such mutein. 47. A method of treating, or ameliorating a disease or disorder associated with deregulated angiogenesis in a subject wherein the disease or disorder is selected from the group consisting of: tumor growth, eye disorders, vascular diseases, inflammatory or infectious diseases, cancer, ocular neovascular diseases, arthritis and psoriasis, comprising administering to said subject an effective amount of an hNGAL mutein of claim 1 or a composition comprising such mutein.

Details for Patent 10,526,382

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Genentech, Inc.	AVASTIN	bevacizumab	Injection	125085	02/26/2004		2035-01-28