Claims for Patent: 10,337,018

Summary for Patent: 10,337,018

Title:	Asparaginase mutant with efficient expression, activity and stability
Abstract:	The present disclosure discloses an asparaginase mutant with efficient expression, activity and stability, belonging to the technical fields of gene engineering and enzyme engineering. The amino acid sequence of the asparaginase mutant is set forth in SEQ ID NO: 99, SEQ ID NO: 100 or SEQ ID NO: 101; meanwhile, compared with the wild type asparaginase mutant, the extracellular enzyme activity of the asparaginase fusion enzyme mutant is increased by up to 2.25 times, the stability is increased by up to 3.56 times, and the specific enzyme activity is increased by up to 1.34 times.
Inventor(s):	Liu; Song (Wuxi, CN), Chen; Jian (Wuxi, CN), Du; Guocheng (Wuxi, CN), Zhao; Weixin (Wuxi, CN)
Assignee:	JIANGNAN UNIVERSITY (Wuxi, CN)
Application Number:	16/177,480
Patent Claims:	1. An asparaginase mutant, wherein the amino acid sequence of the asparaginase mutant is set forth in SEQ ID NO: 99, SEQ ID NO: 100, or SEQ ID NO: 101. 2. A gene encoding the asparaginase mutant of claim 1. 3. A recombinant plasmid comprising the gene of claim 2. 4. The recombinant plasmid of claim 3, wherein the recombinant plasmid is constructed from a pUC, pET or pGEX. 5. The recombinant plasmid of claim 3, wherein the recombinant plasmid is constructed from a pET. 6. A host cell comprising the gene of claim 2. 7. A host cell comprising the recombinant plasmid of claim 3. 8. A method, wherein the method comprises expressing the asparaginase mutant of claim 1 at a predetermined condition.

Details for Patent 10,337,018

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Recordati Rare Diseases, Inc.	ELSPAR	asparaginase	For Injection	101063	01/10/1978		2039-07-18