Details for Patent: 9,820,959
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Title: | Therapeutic compositions |
Abstract: | Ingenol angelate is a potent anticancer agent, and can be stabilized by dissolving it in an aprotic solvent in the presence of an acidic buffer. |
Inventor(s): | Brown; Marc Barry (Watford, GB), Crothers; Michael Edward Donald (Hillsborough, GB), Nazir; Tahir (Middlesex, GB) |
Assignee: | LEO Laboratories Limited (Dublin, IE) |
Filing Date: | May 24, 2016 |
Application Number: | 15/163,390 |
Claims: | 1. A formulation, comprising ingenol angelate, at least about 95% of the ingenol angelate being ingenol-3-angelate (isoform `b`), wherein the formulation is a topical formulation and comprises ingenol-3-angelate (isoform `b`) in an amount from 0.001% by weight to 0.15% by weight and, when an effective amount of the formulation is applied to the skin of a subject, the rate of permeation of the ingenol-3-angelate (isoform `b`) across the skin is between 11 ng cm.sup.-2 h.sup.-1 and 1.92 .mu.g cm.sup.-2 h.sup.-1. 2. The formulation of claim 1, wherein the rate of permeation of the ingenol-3-angelate (isoform `b`) across the skin is between 11 ng cm.sup.-2 h.sup.-1 and 1 .mu.g cm.sup.-2 h.sup.-1. 3. The formulation of claim 1, wherein the amount of ingenol-3-angelate (isoform `b`) applied to the skin is between 0.01 .mu.g cm.sup.-2 and 1 mg cm.sup.-2. 4. The formulation of claim 1, wherein the amount of ingenol-3-angelate (isoform `b`) applied to the skin is between 0.01 .mu.g cm.sup.-2 and 100 .mu.g cm.sup.-1. 5. The formulation of claim 1, further comprising an acidifying agent. 6. The formulation of claim 5, wherein the acidifying agent is an acid buffer. 7. The formulation of claim 6, wherein the acid buffer is selected from the group consisting of a citrate buffer, a phosphate buffer, an acetate buffer, and a citrate-phosphate buffer. 8. The formulation of claim 7, wherein the acid buffer is a citrate buffer. 9. The formulation of claim 6, wherein the formulation comprises from 0.5% by weight to 10% by weight acid buffer. 10. The formulation of claim 1, further comprising a pharmaceutically acceptable solvent, wherein the solvent is selected from the group consisting of polyethylene glycol, methyl ethyl ketone, ethyl acetate, diethyl ether, and benzyl alcohol. 11. The formulation of claim 10, wherein the solvent is benzyl alcohol. 12. The formulation of claim 11, wherein the formulation comprises 0.9% by weight benzyl alcohol. 13. The formulation of claim 1, further comprising a penetration enhancer, wherein the penetration enhancer is selected from the group consisting of isopropyl alcohol, a sulphoxide, an azone, a pyrrolidone, and an alkanol. 14. The formulation of claim 13, wherein the penetration enhancer is isopropyl alcohol. 15. The formulation of claim 14, wherein the formulation comprises 30% by weight isopropyl alcohol. 16. The formulation of claim 1, further comprising a gelling agent. 17. The formulation of claim 16, wherein the gelling agent is selected from the group consisting of a hydroxyalkyl cellulose polymer, carboxymethyl cellulose, methylhydroxyethyl cellulose, methyl cellulose, a carbomer, and a carrageenan. 18. The formulation of claim 17, wherein the gelling agent is hydroxyethylcellulose. 19. The formulation of claim 18, wherein the formulation comprises 1.5% by weight hydroxyethylcellulose. 20. The formulation of claim 17, wherein the formulation comprises from 1% by weight to 5% by weight gelling agent. 21. The formulation of claim 1, wherein the formulation comprises from 0.01% by weight to 0.1% by weight ingenol-3-angelate (isoform `b`). 22. The formulation of claim 1, wherein the formulation has a pH of no greater than 4.5. 23. The formulation of claim 1, wherein the formulation has a pH of no less than 2.5. 24. The formulation of claim 1, wherein ingenol-3-angelate (isoform `b`) is the only active ingredient. 25. The formulation of claim 1, wherein the formulation is selected from the group consisting of a gel, a cream, an ointment, a paint, a lotion, and a foam. 26. The formulation of claim 1, wherein the formulation is sterilized. 27. The formulation of claim 1, wherein the formulation is suitable for storage at 2-8.degree. C. 28. The formulation of claim 1, wherein the formulation is suitable for storage at 2-8.degree. C. for at least one year. 29. A method of treating a condition in a subject in need thereof, the method comprising: topically administering a therapeutically effective amount of the formulation of claim 1 to a skin lesion of the subject, wherein the condition is selected from the group consisting of squamous cell carcinoma, basal cell carcinoma, malignant melanoma, and actinic keratosis. 30. The method of claim 29, wherein the condition is actinic keratosis. 31. The formulation of claim 1, wherein at least about 99% of the ingenol angelate is ingenol-3-angelate (isoform `b`). |