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Claims for Patent: 4,658,021

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Claims for Patent: 4,658,021

Title: Methionyl human growth hormone
Abstract:Described are methods and means for the construction and microbial expression of quasi-synthetic genes arising from the combination of organic synthesis and enzymatic reverse transcription from messenger RNA sequences incomplete from the standpoint of the desired protein product. Preferred products of expression lack bio-inactivating leader sequences common in eukaryotic expression products but problematic with regard to microbial cleavage to yield bioactive material. Illustrative is a preferred embodiment in which a gene coding for human growth hormone (useful in, e.g., treatment of hypopituitary dwarfism) is constructed and expressed.
Inventor(s): Goeddel; David V. (Burlingame, CA), Heyneker; Herbert L. (Burlingame, CA)
Assignee: Genentech, Inc. (So. San Francisco, CA)
Application Number:06/654,340
Patent Claims: 1. Methionyl human growth hormone.
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