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Last Updated: May 29, 2023

Patent: 6,127,139

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Summary for Patent: 6,127,139
Title: Method for assaying proteolytic enzymes using fluorescence-quenched substrates
Abstract:A method is disclosed for assaying a proteolytic enzyme comprising: (a) incubating an enzyme-containing sample with an immobilized fluorescence-quenched peptide having the formula Que-Sub-Flu-Spa-Car or Flu-Sub-Que-Spa-Car wherein Sub is a peptide chain containing a specific cleavage site for said proteolytic enzyme; Flu is a fluorophore; Que is a quencher capable of absorbing fluorescent radiation emitted by the fluorophore; Spa is a direct bond or a spacing chain; and Car is a water-insoluble and/or macromolecular carrier; (b) optionally separating the liquid from the carrier material; (c) irradiating said carrier material and measuring fluorescence. Also disclosed are immobilized substrates containing specific amino acid sequences for use in such an assay, especially in an assay for aggrecanase, and metalloproteinase-1, -3 and -13 activity.
Inventor(s): Te Koppele; Johannes Maria (Leiderdorp, NL), Beekman; Bob (Leiden, NL)
Assignee: Nederlands Organisatle voor Toegepast-Natuurwetenschappelijk Onderzoek (Delft, NL)
Application Number:09/101,167
Patent Claims:see list of patent claims

Details for Patent 6,127,139

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Smith & Nephew, Inc. SANTYL collagenase Ointment 101995 06/04/1965 ⤷  Try a Trial 2016-01-04
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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