Enrichment method for variant proteins having altered binding properties, M13 phagemids, and growth hormone variants
A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.
Garrard; Lisa J. (Burlingame, CA), Henner; Dennis J. (Pacifica, CA), Bass; Steven (Redwood City, CA), Greene; Ronald (Durham, NC), Lowman; Henry B. (Hercules, CA), Wells; James A. (Burlingame, CA), Matthews; David J. (San Francisco, CA)
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