Claims for Patent: RE45660

Summary for Patent: RE45660

Title:	Xylanases, nucleic acids encoding them and methods for making and using them
Abstract:	The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal .beta.-1,4-xylosidic linkages or endo-.beta.-1,4-ghicanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.
Inventor(s):	Weiner; David (Del Mar, CA), Blum; David (Nashville, TN), Varvak; Alexander (San Diego, CA), Healey; Shaun (Carlsbad, CA), Chang; Kristine (San Diego, CA), Hazlewood; Geoff (Newbury, GB), Todaro; Thomas (San Diego, CA), Desantis; Grace (San Diego, CA), Chang; Hwai (San Marcos, CA), Hansen; Connie Jo (San Diego, CA), Beaver; Scott W. (San Marcos, CA), Woodward; Thomas (Scottsville, VA), Hancock; Charles (San Marcos, CA)
Assignee:	BP Corporation North America Inc. (Houston, TX)
Application Number:	14/064,032
Patent Claims:	1. An isolated, synthetic or recombinant nucleic acid comprising: (a) a nucleic acid of SEQ ID NO: 383; wherein the nucleic acid encodes a polypeptide having a xylanase activity (b) the nucleic acid of (a), further comprising modification of the nucleotides at residues 40 to 42, wherein the nucleotides are modified to CAC or CAT and wherein in the nucleic acid encodes a polypeptide having a xylanase activity, or (c) a nucleic acid encoding a polypeptide having a xylanase activity, wherein the polypeptide comprises .[.the.]. .Iadd.a .Iaddend.sequence of SEQ ID NO: 384. 2. The nucleic acid of claim 1 further comprising at least one modification selected from the group consisting of: the nucleotides at residues 10 to 12 are modified to .[.CCT.]. .Iadd.CTT.Iaddend., TTA, TTG, CTC, CTA or CTG, the nucleotides at residues 25 to 27 are modified to CCC, CCG, CCA or CCT, the nucleotides at residues 28 to 30 are modified to TCA, TCC, TCT, TCG, AGT or AGC, the nucleotides at residues 37 to 39 are modified to TTT or TTC, the nucleotides at residues 37 to 39 are modified to TAC or TAT, the nucleotides at residues 37 to 39 are modified to ATA, ATT or ATC, the nucleotides at residues 37 to 39 are modified to TGG, the nucleotides at residues 52 to 54 are modified to TTC or TTT, the nucleotides at residues 73 to 75 are modified to GAG or GAA, the nucleotides at residues 73 to 75 are modified to CCC, CCG, CCA or CCT, the nucleotides at residues 88 to 90 are modified to GTG, GTC, GTA or GTT, the nucleotides at residues 100 to 102 are modified to TGT or TGC, the nucleotides at residues 100 to 102 are modified to CAT or CAC, the nucleotides at residues 100 to 102 are modified to TTG, TTA, CTT, CTC, CTA or CTG, the nucleotides at residues 103 to 105 are modified to GAG or GAA, the nucleotides at residues 103 to 105 are modified to GAT or GAC, the nucleotides at residues 211 to 213 are modified to ACA, ACT, ACC or ACG, the nucleotides at residues 211 to 213 are modified to TGT or TGC, and the nucleotides at residues 508 to 582 are modified to CAT or CAC, wherein said nucleic acid encodes a polypeptide having xylanase activity. 3. An expression cassette, a vector or a cloning vehicle comprising the nucleic acid of claim 1, wherein the cloning vehicle comprises a viral vector, a plasmid, a phage, a phagemid, a cosmid, a fosmid, a bacteriophage or an artificial chromosome, wherein the viral vector comprises an adenovirus vector, a retroviral vector or an adeno-associated viral vector, or, the artificial chromosome comprises a bacterial artificial chromosome (BAC), a bacteriophage P 1-derived vector (PAC), a yeast artificial chromosome (YAC), or a mammalian artificial chromosome (MAC). 4. A transformed isolated host cell comprising the nucleic acid of claim 1, or comprising an expression cassette, a vector or a cloning vehicle as set forth in claim 3, wherein the host cell is a bacterial cell, a mammalian cell, a fungal cell, a yeast cell, an insect cell or a plant cell. 5. A transgenic non-human animal, plant, plant part or plant seed comprising the nucleic acid of claim 1, wherein the plant is a corn plant, a sorghum plant, a potato plant, a tomato plant, a wheat plant, an oilseed plant, a rapeseed plant, a soybean plant, a rice plant, a barley plant, a grass, a cotton plant, a cottonseed plant, a palm, a sesame plant, a peanut plant, a sunflower plant or a tobacco plant. 6. A method of producing a recombinant polypeptide comprising (a) providing the nucleic acid of claim 1 or claim 2; and (b) expressing the nucleic acid of (a) under conditions that allow expression of the polypeptide, thereby producing a recombinant polypeptide, and the method further comprises transforming an isolated host cell with the nucleic acid of (a) followed by expressing the nucleic acid of (a), thereby producing a recombinant polypeptide in a transformed isolated host cell, or (II) (a) providing a vector comprising the nucleic acid of claim 1 or claim 2; and (b) expressing the vector of (a), wherein expression is effected by use of a high activity promoter, a dicistronic vector or by gene amplification of the vector.

Details for Patent RE45660

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Aimmune Therapeutics, Inc.	PALFORZIA	peanut (arachis hypogaea) allergen powder	Powder	125696	01/31/2020		2040-02-22