Claims for Patent: 9,757,435

Summary for Patent: 9,757,435

Title:	Clostridium histolyticum enzymes and methods for the use thereof
Abstract:	The invention relates to recombinant nucleic acid and polypeptides encoding collagenase I and collagenase II, methods for the preparation thereof and methods for the use thereof. The invention also encompasses methods related to releasing a composition comprising collagenase prior to therapeutic administration.
Inventor(s):	Herber; Wayne K. (Coopersburg, PA)
Assignee:	ENDO GLOBAL VENTURES (Hamilton, BM)
Application Number:	14/328,772
Patent Claims:	1. A recombinant nucleic acid molecule comprising; a) a polynucleotide having the nucleic acid sequence of SEQ ID NO: 1 or the complement of SEQ ID NO: 1, and b) a heterologous regulatory sequence operably linked to the polynucleotide. 2. The recombinant nucleic acid molecule of claim 1, wherein the heterologous regulatory sequence is a heterologous promoter operably linked to the nucleic acid sequence of SEQ ID NO: 1 or to the complement of SEQ ID NO: 1. 3. A vector comprising the nucleic acid molecule of claim 1. 4. The vector of claim 3, wherein the vector is a plasmid. 5. A recombinant host cell comprising the vector of claim 3. 6. The recombinant host cell of claim 5, wherein the host cell is selected from the group consisting of a bacterial cell, a fungal cell, an insect cell, a plant cell and a mammalian cell. 7. The recombinant host cell of claim 6, wherein the host cell is selected from the group consisting of E. coli, Streptomyces, Pseudomonas, Serratia marcescens, Salmonella typhimurium, a yeast cell, plant cells, thymocytes, Chinese hamster ovary cell (CHO), COS cell, and Lactococcus lacti. 8. A method of producing collagenase I comprising culturing the cell of claim 5 under conditions suitable for expression of the nucleic acid and recovering the collagenase I. 9. The recombinant nucleic acid molecule of claim 1, wherein the recombinant nucleic acid molecule is fused to a marker sequence. 10. The recombinant nucleic acid molecule of claim 9, wherein the marker sequence encodes a polypeptide selected from the group consisting of: a glutathione-S-transferase (GST) fusion protein, a hemaglutin A (HA) polypeptide marker from influenza, and hexa-histidine peptide. 11. The vector of claim 3, wherein the vector is introduced into a host cell using conventional transformation or transfection techniques. 12. The recombinant nucleic acid molecule of claim 1 having a biological activity of a naturally-occurring collagenase.

Details for Patent 9,757,435

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2032-01-12