Claims for Patent: 9,650,641

Summary for Patent: 9,650,641

Title:	Zea mays promoter from chlorophyll a/b binding protein gene and uses thereof
Abstract:	Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays chlorophyll a/b binding gene regulatory elements.
Inventor(s):	Gupta; Manju (Carmel, IN), Bennett; Sara (Indianapolis, IN), Elango; Navin (Indianapolis, IN), Muthuraman; Karthik (Indianapolis, IN), Beringer; Jeffrey (Indianapolis, IN), Wu; Huixia (Indianapolis, IN)
Assignee:	Dow AgroSciences LLC (Indianapolis, IN)
Application Number:	14/604,510
Patent Claims:	1. A gene expression cassette comprising a promoter operably linked to a heterologous nucleic acid, wherein the promoter comprises a polynucleotide comprising a sequence identity of at least 90% to SEQ ID NO:2. 2. The gene expression cassette of claim 1, wherein the polynucleotide has at least 95% sequence identity to SEQ ID NO:2. 3. The gene expression cassette of claim 1, wherein the polynucleotide comprises an intron. 4. The gene expression cassette of claim 3, wherein the intron has at least 90% sequence identity to SEQ ID NO:5. 5. the gene expression cassette of claim 1, wherein the polynucleotide has at least 90% sequence identity to SEQ D NO: 1. 6. The gene expression cassette of claim 1, wherein the operably linked nucleic acid encodes a polypeptide or a small RNA. 7. The gene expression cassette of claim 1, wherein the nucleic acid is selected from the group consisting of a nucleic acid conferring insecticidal resistance, a nucleic acid conferring herbicide tolerance, a nucleic acid conferring nitrogen use efficiency, a nucleic acid conferring water use efficiency, a nucleic acid conferring nutritional quality, a nucleic acid encoding a DNA binding protein, and a nucleic acid encoding a selectable marker. 8. The gene expression cassette of claim 1 further comprising a 3'-untranslated region. 9. The gene expression cassette of claim 8, wherein the 3'-untranslated region has at least 90% sequence identity to SEQ ID NO:7 or SEQ ID NO;8. 10. The gene expression cassette of claim 1 further comprising a 5'-untranslated regions, wherein the 5'-UTR has at least 90% sequence identity to SEQ ID NO:19 . 11. A recombinant vector comprising the gene expression cassette of claim 1 ,wherein the vector is selected from the group consisting of a plasmid, a cosmid, a bacterial artificial chromosome, a virus, and a bacteriophage. 12. A transgenic cell comprising the gene expression cassette of claim 1. 13. The transgenic cell of claim 12, wherein the transgenic cell is a transgenic plant cell. 14. A transgenic plant comprising the transgenic plant cell of claim 13. 15. The transgenic plant of claim 14, wherein the transgenic plant is a monocotyledonous plant or dicotyledonous plant. 16. The transgenic plant of claim 15, wherein the monocotyledonous plant is selected from the group consisting of a maize plant, a rice plant, and a wheat plant. 17. A transgenic seed from the transgenic plant of claim 14, wherein the seed comprises the gene expression cassette. 18. The gene expression cassette of claim 1, wherein the promoter is a tissue-preffered promoter. 19. The gene expression cassette of claim 1, wherein the tissue-preferred promoter is a leaf, husk, stem or silk tissue-preferred promoter. 20. The gene expression cassette of claim 1, wherein the promoter comprises the polynucleotide sequence of nucleotides 1-1,887 of SEQ ID NO: 2. 21. A method for expressing a coding sequence in a transgenic plant, the method comprising: a) transforming a plant cell with a gene expression cassette comprising a polynucleotide sequence comprising a sequence identity of at least 90% to SEQ ID NO:2 operably linked to a heterologous coding sequence, which is operably linked to a 3'-untranslated region; b) isolating the transformed plant cell comprising the gene expression cassette; c) regenerating a transgenic plant from the transformed plant cell; and, d) obtaining the transgenic plant, wherein the transgenic plant expresses the coding sequence. 22. A method for manufacturing a synthetic polynucleotide sequence comprising a sequence identity of at least 90% to SEQ ID NO:2, the method comprising: a) isolating a nucleic acid comprising a polynucleotide sequence comprising SEQ ID NO:2; b) producing a plurality of oligonucleotide primer sequences, wherein the oligonucleotide primer sequences bind to the nucleic acid under stringent hybridization conditions; c) ligating the plurality of oligonucleotide primer sequences to synthesize a synthetic polynucleotide sequence; and, d) sequencing the resulting synthetic polynucleotide to confirm that it comprises at least 90% identity to SEQ ID NO:2.

Details for Patent 9,650,641

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2034-01-23
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2034-01-23
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2034-01-23