Claims for Patent: 9,458,466
✉ Email this page to a colleague
Summary for Patent: 9,458,466
Title: | Translational control system using RNA-protein interaction motif |
Abstract: | A translational control method using an RNA-protein interaction motif is provided. The method comprises a step of introducing an mRNA having: a 5\'UTR regulation structure comprising: (1) a cap structure at the 5\' terminus, (2) a spacer positioned on the 3\' side of the cap structure, and (3) one or more RNA motifs positioned on the 3\' side of the spacer, which comprises an RNA-protein interaction motif-derived nucleotide sequence or a variant thereof; and a nucleotide sequence encoding a target protein gene on the 3\' side of the 5\'UTR regulation structure, into a cell in the presence of a protein specifically binding to the RNA motifs, wherein a translational level is decreased as the number of bases of the spacer decreases, and the translational level is decreased as the number of the RNA motifs increases. |
Inventor(s): | Saito; Hirohide (Kyoto, JP), Endo; Kei (Kyoto, JP), Inoue; Tan (Kyoto, JP) |
Assignee: | Kyoto University (Kyoto, JP) |
Application Number: | 14/415,009 |
Patent Claims: | 1. An mRNA comprising: a 5' UTR regulation structure comprising: (1) a cap structure at the 5' terminus; (2) one or more RNA motifs positioned on the 3' side of the
cap structure, of an RNA-protein interaction motif-derived nucleotide sequence or a variant thereof; and (3) an ON switch cassette, said ON switch cassette comprising (a) a bait open reading frame (a bait ORF), (b) an intron comprising a human beta
globin intron or a chimeric intron, and (c) an internal ribosome entry site (IRES); and a nucleotide sequence positioned on the 3' side of the 5' UTR regulation structure and having a nucleotide sequence encoding a gene of a target protein, wherein the
bait ORF is a sequence comprising a stop codon in more than 320 bases from the intron; wherein said ON switch cassette is positioned on (a) 3' side of the RNA motif if the 5' UTR regulation structure comprises one RNA motif structure; or (b) 3' side of
the RNA motif positioned at 3' end of the RNA motifs if the 5' UTR regulation structure comprises two or more RNA motifs; wherein the mRNA is an artificially prepared non-natural mRNA; wherein said human beta globin intron comprises a nucleotide
sequence of Seq. ID No. 18; wherein said chimeric intron comprises a nucleotide sequence of Seq. ID No. 83.
2. The mRNA according to claim 1, wherein the bait ORF is a sequence comprising a stop codon inserted at the 457th and/or 466th base from the 5' side of Renilla luciferase; wherein said Renilla luciferase comprising a nucleotide sequence of Seq. ID No. 17 or Seq. ID No. 81. 3. The mRNA according to claim 1, wherein the intron is human .beta. globin intron. 4. The mRNA according to claim 1, wherein each of the RNA motifs is a binding sequence selected from the group consisting of a binding sequence of L7Ae protein, a binding sequence of MS2 phage coat protein and a binding sequence of Bacillus stearothermophilus ribosomal protein S15. |
Details for Patent 9,458,466
Applicant | Tradename | Biologic Ingredient | Dosage Form | BLA | Approval Date | Patent No. | Expiredate |
---|---|---|---|---|---|---|---|
Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | For Injection | 103132 | 06/04/1986 | ⤷ Try a Trial | 2032-07-16 |
Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | For Injection | 103132 | ⤷ Try a Trial | 2032-07-16 | |
Merck Sharp & Dohme Corp. | INTRON A | interferon alfa-2b | Injection | 103132 | ⤷ Try a Trial | 2032-07-16 | |
>Applicant | >Tradename | >Biologic Ingredient | >Dosage Form | >BLA | >Approval Date | >Patent No. | >Expiredate |
Make Better Decisions: Try a trial or see plans & pricing
Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.