Get our Free Patent Expiration Newsletter

Serving leading biopharmaceutical companies globally:

McKesson
Baxter
Colorcon
Dow
AstraZeneca
Johnson and Johnson

Last Updated: January 23, 2020

DrugPatentWatch Database Preview

Claims for Patent: 9,273,320

Join the DrugPatentWatch Referral Program
Get access to a free drug patent landscape report or a free one-month subscription

« Back to Dashboard

Summary for Patent: 9,273,320
Title:Method of producing a sweet protein
Abstract: The present invention relates to a method of producing a recombinant sweet protein in a filamentous fungus. The invention also relates to isolated polynucleotides encoding the sweet protein and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, as well as methods of using the sweet protein produced by the method of the invention.
Inventor(s): Vind; Jesper (Vaerloese, DK), Tams; Jeppe Wegener (Gentofe, DK), Beier; Lars (Lyngby, DK), Olsen; Carsten Lillelund (Bagsvaerd, DK)
Assignee: NOVOZYMES A/S (Bagsvaerd, DK)
Application Number:13/382,535
Patent Claims:1. A method of producing a recombinant sweet protein comprising an amino acid sequence having at least 90% identity to amino acids 2 to 54 of SEQ ID NO: 2, which comprises: a) cultivating an Aspergillus host cell comprising a nucleic acid construct comprising a polynucleotide encoding the sweet protein under conditions conducive for production of the sweet protein; and b) recovering the sweet protein wherein the sweet protein is secreted from the host cell in a functional form.

2. A method according to claim 1, wherein the sweet protein is brazzein or a variant of brazzein.

3. A method according to claim 1, wherein the host cell is an Aspergillus oryzae host cell or an Aspergillus niger host cell.

4. A method according to claim 1, wherein the nucleic acid construct comprises the polynucleotide encoding the sweet protein operably linked to a first nucleotide sequence encoding a signal peptide comprising or consisting of an amino acid sequence having at least 90% identity to amino acids -55 to -33 of SEQ ID NO: 2, and a second nucleotide sequence encoding a propeptide comprising or consisting of an amino acid sequence having at least 90% identity to amino acids -32 to -1 of SEQ ID NO: 2.

5. A method according to claim 4, wherein the sweet protein is recovered in a form where the signal peptide and the propeptide have been cleaved off.

6. A method according to claim 1, wherein the nucleic acid construct comprises one or more intron sequence(s).

7. The method of claim 1, wherein the recombinant sweet protein comprises an amino acid sequence having at least 95% identity to amino acids 2 to 54 of SEQ ID NO: 2.

8. The method of claim 1, wherein the recombinant sweet protein comprises an amino acid sequence having at least 97% identity to amino acids 2 to 54 of SEQ ID NO: 2.

9. The method of claim 1, wherein the recombinant sweet protein comprises an amino acid sequence having at least 98% identity to amino acids 2 to 54 of SEQ ID NO: 2.

10. The method of claim 1, wherein the recombinant sweet protein comprises an amino acid sequence having at least 99% identity to amino acids 2 to 54 of SEQ ID NO: 2.

11. The method of claim 1, wherein the recombinant sweet protein comprises the amino acid sequence shown as amino acids 2 to 54 of SEQ ID NO: 2.

12. The method of claim 1, wherein the recombinant sweet protein consists of the amino acid sequence shown as amino acids 2 to 54 of SEQ ID NO: 2.

13. A method of increasing the sweetness of a food, beverage or medication product for human consumption comprising the step of adding a sufficient amount of a sweet protein to the product, so that the product has an increased sweetness, wherein the sweet protein has been produced according to the method of claim 1.

14. A food, beverage or medication product for human consumption which comprises a sweet protein which has been produced according to the method of claim 1.

Summary for Patent:   Start Trial

Foriegn Application Priority Data
Foreign Country Foreign Patent Number Foreign Patent Date
09167464Aug 7, 2009
PCT Information
PCT FiledAugust 05, 2010PCT Application Number:PCT/EP2010/061435
PCT Publication Date:February 10, 2011PCT Publication Number:WO2011/015633

Details for Patent 9,273,320

Applicant Tradename Biologic Ingredient Dosage Form BLA Number Approval Date Patent No. Assignee Estimated Patent Expiration Status Orphan Source
Schering INTRON A interferon alfa-2b VIAL 103132 001 1986-06-04   Start Trial NOVOZYMES A/S (Bagsvaerd, DK) 2029-08-07 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 002 1986-06-04   Start Trial NOVOZYMES A/S (Bagsvaerd, DK) 2029-08-07 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 003 1986-06-04   Start Trial NOVOZYMES A/S (Bagsvaerd, DK) 2029-08-07 RX search
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Number >Approval Date >Patent No. >Assignee >Estimated Patent Expiration >Status >Orphan >Source

Make Better Decisions: Try a trial or see plans & pricing

Serving leading biopharmaceutical companies globally:

Medtronic
Baxter
Colorcon
Dow
AstraZeneca
McKinsey

Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verifification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.