Make Better Decisions - Finding and Evaluating Generic and Branded Drug Market Entry Opportunities

Get the Book: Make Better Decisions

Finding and Evaluating Generic and Branded Drug Market Entry Opportunities

PDF eBook: Just $10 Get Print Book on Amazon

Serving hundreds of leading biopharmaceutical companies globally:

Merck
Fish and Richardson
Harvard Business School
Argus Health
UBS
Medtronic

Generated: August 21, 2019

DrugPatentWatch Database Preview

Claims for Patent: 9,181,310

  Try a free trial


See Plans and Pricing

« Back to Dashboard

Summary for Patent: 9,181,310
Title:Use of bacteriophage outer membrane breaching proteins expressed in plants for the control of gram-negative bacteria
Abstract: The present invention provides compositions and methods for killing or suppressing growth of Gram-negative bacteria that infect, infest or cause disease in plants, including pathogenic, saprophytic and opportunistic microbes that cause disease in plants and food borne illness in people or in animal feed.
Inventor(s): Gabriel; Dean W. (Alachua, FL), Jiang; Yingnan (Alachua, FL)
Assignee: University of Florida Research Foundation, Inc. (Gainesville, FL) Integrated Plant Genetics, Inc. (Gainesville, FL)
Application Number:13/801,088
Patent Claims:1. A transgenic plant, plant part, plant cell, or plant tissue culture comprising a DNA molecule, wherein the DNA molecule encodes a Bacterial Outer Membrane Breaching (BOMB) polypeptide sharing at least 90% amino acid identity with a BOMB polypeptide selected from the group consisting of SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, and SEQ ID NO:17.

2. The transgenic plant, plant part, plant cell, or plant tissue culture of claim 1, wherein the DNA molecule encodes a BOMB polypeptide sharing at least 95% amino acid identity with a BOMB polypeptide selected from the group consisting of SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, and SEQ ID NO:17.

3. The transgenic plant, plant part, plant cell, or plant tissue culture of claim 1, wherein the DNA encoding the BOMB polypeptide is any codon-optimized version of said DNA.

4. A method for enhancing resistance of a plant to infection or infestation by Gram-negative bacteria, said method comprising introducing into the genome of the plant an expression cassette comprising: 1) a plant promoter; 2) a gene comprising a nucleic acid sequence selected from the group consisting of (a) a nucleic acid sequence encoding a BOMB polypeptide sharing at least 90% amino acid identity with a BOMB polypeptide selected from the group consisting of SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, and SEQ ID NO:17; and (b) any codon optimized version of a nucleic acid of a sequence of (a), wherein the nucleic acid sequence is operably fused to said promoter; and 3) a plant terminator.

5. The method of claim 4, wherein the Gram-negative bacteria are pathogenic.

6. The method of claim 4, wherein the expression cassette further comprises a nucleic acid sequence encoding a secretion signal and/or an intron.

7. The method of claim 4 wherein the expression cassette further comprises an endoplasmic reticulum (ER) retention signal.

8. The method of claim 4, wherein said BOMB polypeptide is used in combination with, whether separately cloned and transformed or not, whether operably fused with or not, an additional protein, polypeptide, or peptide fragment selected from the group consisting of: (1) a nonenzymatic lytic peptide or peptide fragment, (2) an enzymatic lytic peptide or peptide fragment or protein, and (3) an enzymatic peptidoglycan degrading peptide or peptide fragment.

9. The method of claim 8, wherein the additional protein, polypeptide, or peptide fragment is selected from the group consisting of lysozymes, endolysins, proteases, chitinases, mureinolytic enzymes, enzymes with transglycosylase activity, lipases and esterases, and functional fragments thereof.

10. The plant, plant part, plant cell, or plant tissue culture of claim 3, wherein the plant is a dicot plant or a monocot plant.

11. The plant, plant part, plant cell, or plant tissue culture of claim 3, wherein the plant is selected from the group consisting of geranium plants, citrus plants, tobacco plants, and rice plants.

12. Progeny of the plant of claim 3, wherein the progeny comprises the DNA molecule encoding the BOMB polypeptide.

13. The transgenic plant, plant part, plant cell, or plant tissue culture of claim 1, wherein the DNA molecule encoding the BOMB polypeptide comprises a plant intron.

14. The transgenic plant, plant part, plant cell, or plant tissue culture of claim 1, wherein the BOMB polypeptide originates from a bacteriophage.

15. The transgenic plant, plant part, plant cell, or plant tissue culture of claim 1, wherein the BOMB polypeptide has the following properties: (a) originating from a bacteriophage; (b) lacking a bacterial secretion signal sequence; (c) lacking a functional alphahelical transmembrane domain; (d) contains a beta strand-linker-beta strand domain, wherein the domain is predicted to localize in an outer membrane of a bacterium when contacted with the bacterium; and (e) contains a globular domain.

16. The method of claim 4, wherein the BOMB polypeptide shares at least 90% or at least 95% amino acid identity with a BOMB polypeptide selected from the group consisting of SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, and SEQ ID NO:17.

Details for Patent 9,181,310

Applicant Tradename Biologic Ingredient Dosage Form BLA Number Approval Date Patent No. Assignee Estimated Patent Expiration Status Orphan Source
Schering INTRON A interferon alfa-2b VIAL 103132 001 1986-06-04   Try a Free Trial University of Florida Research Foundation, Inc. (Gainesville, FL) Integrated Plant Genetics, Inc. (Gainesville, FL) 2023-05-14 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 002 1986-06-04   Try a Free Trial University of Florida Research Foundation, Inc. (Gainesville, FL) Integrated Plant Genetics, Inc. (Gainesville, FL) 2023-05-14 RX search
Schering INTRON A interferon alfa-2b VIAL 103132 003 1986-06-04   Try a Free Trial University of Florida Research Foundation, Inc. (Gainesville, FL) Integrated Plant Genetics, Inc. (Gainesville, FL) 2023-05-14 RX search
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Number >Approval Date >Patent No. >Assignee >Estimated Patent Expiration >Status >Orphan >Source

Subscribe to access the full database, or try a Free Trial

Make Better Decisions: Try a trial or see plans & pricing

Serving hundreds of leading biopharmaceutical companies globally:

US Army
Medtronic
Express Scripts
McKesson
Fuji
Cipla

Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verifification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.