Claims for Patent: 8,993,303

Summary for Patent: 8,993,303

Title:	Genetically engineered cyanobacteria
Abstract:	The disclosed embodiments provide cyanobacteria spp. that have been genetically engineered to have increased production of carbon-based products of interest. These genetically engineered hosts efficiently convert carbon dioxide and light into carbon-based products of interest such as long chained hydrocarbons. Several constructs containing polynucleotides encoding enzymes active in the metabolic pathways of cyanobacteria are disclosed. In many instances, the cyanobacteria strains have been further genetically modified to optimize production of the carbon-based products of interest. The optimization includes both up-regulation and down-regulation of particular genes.
Inventor(s):	Zhou; Ruanbao (Brookings, SD), Gibbons; William (Brookings, SD)
Assignee:	South Dakota State University (Brookings, SD)
Application Number:	13/405,208
Patent Claims:	1. A composition comprising an Anabaena spp. genetically engineered with at least one recombinant polynucleotide expression construct, wherein the at least one recombinant polynucleotide expression construct comprises a nucleotide sequence encoding at least one enzyme, wherein the at least one enzyme increases production of a carbon based product of interest by the genetically engineered Anabaena spp. following expression of the polynucleotide expression construct, wherein said Anabaena spp. is ethanol producing Anabaena sp. PCC7120 (pZR672) strain deposited under ATCC accession number PTA-12833 or is linalool producing Anabaena sp. PCC7120 (pZR808) strain deposited under ATCC accession number PTA-12832. 2. The composition of claim 1 wherein the Anabaena spp. is Anabaena PCC7120 (pZR672) strain deposited under ATCC accession number PTA-12833. 3. The composition of claim 1, wherein the Anabaena spp. is linalool producing Anabaena sp. PCC7120 (pZR808) strain deposited under ATCC accession number PTA-12832. 4. The composition of claim 1 wherein the Anabaena spp. has an up-regulated 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. 5. The composition of claim 4 wherein the up-regulated MEP pathway is up-regulated by expressing at least one gene responsible for control of the MEP pathway in the Anabaena spp. 6. The composition of claim 1 wherein the at least one recombinant polynucleotide expression construct further comprises a nucleotide sequence encoding ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo). 7. The composition of claim 6 wherein the at least one recombinant polynucleotide expression construct comprising a nucleotide sequence encoding RuBisCo, further comprises a nucleotide sequence encoding RuBisCo activase. 8. The composition of claim 1 wherein the carbon based product of interest is ethanol. 9. The composition of claim 1 wherein the Anabaena spp. is combined with a photoautotrophic liquid media, and optionally, wherein said media contains no combined nitrogen. 10. The composition of claim 1 wherein the carbon based product of interest is linalool (C.sub.10H.sub.18O).

Details for Patent 8,993,303

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Genentech, Inc.	ACTIVASE	alteplase	For Injection	103172	11/13/1987		2031-02-24
Genentech, Inc.	CATHFLO ACTIVASE	alteplase	For Injection	103172	09/04/2001		2031-02-24