Claims for Patent: 8,846,064

Summary for Patent: 8,846,064

Title:	Cosmetic and pharmaceutical compositions having modified proteins in the form of a supramolecular assembly
Abstract:	A cosmetic and pharmaceutical formulation for rejuvenation and restoration of the skin, and methods of making this formulation. The cosmetic and pharmaceutical formulations comprise liposomes containing partially acylated proteins with a modification level of 0.1 to 10% of their mass, wherein the proteins are present in the form of a supramolecular assembly.
Inventor(s):	Martynov; Artur (Kharkov, UA), Farber; Boris S. (Brooklyn, NY), Farber; Sonya Sophya (New York, NY), Sitenko; Aleksandr (Khar\'kovskaya oblast, UA)
Assignee:
Application Number:	12/931,463
Patent Claims:	1. A Cosmetic and pharmaceutical composition comprising liposomes with proteins, wherein the proteins are partially acylated at a modification level of 0.1 to 10% of their mass and are present in the form of a supramolecular assembly, wherein the partially acylated proteins are derived from at least one of collagenase, hyaluronidase, insulin and mixtures thereof. 2. The composition according to claim 1, wherein the partially acylated proteins are derived from hyaluronidase. 3. The composition according to claim 1, wherein the partially acylated proteins are derived from collagenase. 4. The composition according to claim 1, wherein the partially acylated proteins are derived from insulin. 5. The composition according to claim 1, wherein the partially acylated proteins are derived from a mixture of collagenase, hyaluronidase, and insulin. 6. The composition according to claims 1, wherein the partially acylated proteins are produced via reaction of succinic anhydride and proteins. 7. The composition according to claim 1, wherein the liposomes are obtained by a reverse phase method, followed by an ultrasound processing of the liposomes. 8. The composition according to claim 1, wherein the liposomes are obtained by a freeze-thawing method. 9. The composition according to claim 1, wherein the liposomes are obtained by a method of introducing the protein solution to a suspension of lyophilized liposomes. 10. The composition according to claim 1, wherein the liposomes are derived from phosphatidylcholine. 11. The composition according to claim 1, wherein the liposomes are derived from phosphatidylethanolamine. 12. The composition according to claim 1, wherein the liposomes are derived from a mixture of phosphatidylcholine and phosphatidylethanolamine. 13. A method of making the cosmetic and pharmaceutical composition of claim 1, comprising the partial acylation of proteins with a modification level of 0.1 to 10% of their mass and are present in the form of a supramolecular assembly, and incorporating the partially acylated proteins into liposomes, wherein the partially acylated proteins are derived from at least one of collagenase, hyaluronidase, insulin and mixtures thereof. 14. The method according to claim 13, wherein the partially acylated proteins are derived from hyaluronidase. 15. The method according to claim 13, wherein the partially acylated proteins are derived from collagenase. 16. The method according to claim 13, wherein the partially acylated proteins are derived from insulin. 17. The method according to claim 13, wherein the partially acylated proteins are derived from a mixture of collagenase, hyaluronidase and insulin. 18. The method according to claim 13, wherein the partially acylated proteins are produced via a reaction with succinic anhydride. 19. The method according to claim 13, wherein the liposomes are obtained by a reverse phase method, followed by an ultrasound treatment of the liposomes. 20. The method according to claim 13, wherein the liposomes are obtained by freezing-thawing. 21. The method according to claim 13, wherein the liposomes are obtained by introducing a protein solution into a suspension of lyophilized liposomes. 22. The method according to claims 13, wherein the liposomes are derived from phosphatidylcholine. 23. The method according to claim 13, wherein the liposomes are derived from phosphatidylethanolamine. 24. The method according to claim 13, wherein the liposomes are derived from a mixture of phosphatidylcholine and phosphatidylethanolamine.

Details for Patent 8,846,064

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Bausch & Lomb Incorporated	VITRASE	hyaluronidase	Injection	021640	05/05/2004		2030-11-22
Bausch & Lomb Incorporated	VITRASE	hyaluronidase	Injection	021640	12/02/2004		2030-11-22
Amphastar Pharmaceuticals, Inc.	AMPHADASE	hyaluronidase	Injection	021665	10/26/2004		2030-11-22
Akorn, Inc.	HYDASE	hyaluronidase	Injection	021716	10/25/2005		2030-11-22