Claims for Patent: 8,273,959

Summary for Patent: 8,273,959

Title:	Corn event PV-ZMGT32(nk603) and compositions and methods for detection thereof
Abstract:	The present invention provides a DNA construct that confers tolerance to transgenic corn plant. Also provided are assays for detecting the presence of the PV-ZMGT32(nk603) corn event based on the DNA sequence of the recombinant construct inserted into the corn genome and of genomic sequences flanking the insertion site.
Inventor(s):	Behr; Carl Frederick (Wildwood, MO), Heck; Gregory R. (Crystal Lake Park, MO), Hironaka; Catherine Hironaka (Dublin, CA), You; Jinsong (Ballwin, MO)
Assignee:	Monsanto Technology LLC (St. Louis, MO)
Application Number:	12/460,523
Patent Claims:	1. A corn plant comprising (a) a DNA construct of comprising: a first and a second expression cassette, wherein said first expression cassette in operable linkage comprises (i) a rice actin 1 promoter; (ii) a rice actin 1 intron; (iii) a chloroplast transit peptide encoding DNA molecule; (iv) a glyphosate tolerant EPSPS encoding DNA molecule; and (v) a transcriptional terminator DNA molecule; and said second expression cassette comprising in operable linkage (a) a CaMV 35S promoter; (b) a Hsp70 intron; (c) a chloroplast transit peptide encoding DNA molecule; (d) a glyphosate tolerant EPSPS encoding DNA molecule; and (e) a transcriptional terminator DNA molecule; and (b) a DNA molecule comprising at least two sequences selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11 and SEQ ID NO:12. 2. A method of producing a corn plant that tolerates application of glyphosate herbicide comprising: (a) transforming a corn cell with a DNA construct comprising a first and a second expression cassette, wherein said first expression cassette in operable linkage comprises (i) a rice actin 1 promoter; (ii) a rice actin 1 intron; (iii) a chloroplast transit peptide encoding DNA molecule; (iv) a glyphosate tolerant EPSPS encoding DNA molecule; and (v) a transcriptional terminator DNA molecule; and said second expression cassette comprising in operable linkage (a) a CaMV 35S promoter; (b) a Hsp70 intron; (c) a chloroplast transit peptide encoding DNA molecule; (d) a glyphosate tolerant EPSPS encoding DNA molecule; and (e) a transcriptional terminator DNA molecule; (b) selecting said corn cell with tolerance to application of glyphosate, the cell comprising a DNA molecule comprising at least two sequences selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11 and SEQ ID NO:12; (c) growing said corn cell into a fertile corn plant. 3. A corn plant, seed, nucleus, or part thereof capable of producing a PV-ZMGT32(nk603) diagnostic amplicon. 4. The corn plant, seed, nucleus, or part thereof of claim 3, wherein the PV-ZMGT32(nk603) diagnostic amplicon comprises SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, or SEQ ID NO:12. 5. A corn plant, seed, nucleus, or part thereof comprising corn event PV-ZMGT32(nk603). 6. A glyphosate tolerant corn plant, or DNA-containing part thereof, comprising incorporated into the plant's genome a DNA insert encoding EPSPS from two copies of an EPSPS-encoding gene, wherein the sequence overlapping the junction between the corn genomic DNA and the 5' end of the insert comprises SEQ ID NO:7 and the sequence overlapping the junction between the corn genomic DNA and the 3' end of the insert comprises SEQ ID NO:8.

Details for Patent 8,273,959

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2020-06-22
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2020-06-22
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2020-06-22