Claims for Patent: 8,067,171

Summary for Patent: 8,067,171

Title:	Methods and compositions for improved fertilization and embryonic survial
Abstract:	Single nucleotide polymorphic site at position 11646 of the bovine FGF2 gene is associated with improved fertilization rate and/or improved embryo survival rate, as well as improved milk production. Also disclosed are nucleic acid molecules, kits, methods of genotyping and marker assisted bovine breeding methods.
Inventor(s):	Khatibm; Hasan (Madison, WI)
Assignee:	Wisconsin Alumni Research Foundation (Madison, WI)
Application Number:	12/424,796
Patent Claims:	1. A method for detecting a single nucleotide polymorphism in the bovine FGF2 gene, wherein the FGF2 gene comprises SEQ ID NO: 1, the method comprising: detecting, in a nucleic acid sample from a bovine, the presence of a G nucleotide in intron 1 of the FGF2 gene at the position corresponding to position 1326 of SEQ ID NO: 1. 2. A method for genotyping a bovine cell, comprising obtaining a nucleic acid sample from said cell and detecting the presence of a G nucleotide in intron 1 of the FGF2 gene at the position corresponding to position 1326 of SEQ ID NO: 1. 3. The method of claim 2, wherein the bovine cell is an adult cell, an embryo cell, a sperm, an egg, a fertilized egg, or a zygote. 4. The method of claim 2, wherein the G nucleotide is detected by sequencing the FGF2 gene, or a fragment thereof, isolated from the cell. 5. The method of claim 4, wherein the gene or a fragment thereof is isolated from the cell via amplification by polymerase chain reaction (PCR) of genomic DNA of the cell, or by RT-PCR of suitable mRNA of the cell. 6. The method of claim 4, wherein both copies of the FGF2 gene in the cell are genotyped. 7. A method for progeny testing of cattle, the method comprising collecting a nucleic acid sample from said progeny, and genotyping said nucleic sample according to the method of claim 2. 8. A method for selectively breeding of cattle using a multiple ovulation and embryo transfer (MOET) procedure, the method comprising superovulating a female bovine animal, collecting eggs from said superovulated female, in vitro fertilizing said eggs using sperm from a suitable male animal, implanting said fertilized eggs into other females allowing for an embryo to develop, and genotyping said developing embryo according to the method of claim 2, and terminating pregnancy if the developing embryo does not have a G nucleotide in intron 1 of the FGF2 gene at the position corresponding to position 1326 of SEQ ID NO: 1. 9. A method for selectively breeding dairy cattle, comprising selecting a bull that is homozygous for the presence of a G nucleotide in intron 1 of the FGF2 gene at a position corresponding to position 1326 of SEQ ID NO: 1 and using its semen for fertilizing a female animal. 10. The method of claim 9, wherein the female animal is in vitro fertilized. 11. The method of claim 9, wherein said female animal is homozygous for the presence of a G nucleotide in intron 1 of the FGF2 gene at a position corresponding to position 1326 of SEQ ID NO: 1. 12. A method for identifying a dairy cattle as having an increased embryo survival rate, said method comprising: detecting in a nucleic acid sample from said cattle the presence of a G nucleotide in intron 1 of the both copies of the FGF2 gene at the position corresponding to position 1326 of SEQ ID NO: 1; wherein the presence of a G nucleotide in intron 1 of the both copies of the FGF2 gene at the position corresponding to position 1326 of SEQ ID NO: 1 is indicative of an increased embryo survival rate.

Details for Patent 8,067,171

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2029-04-16
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2029-04-16
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2029-04-16