Claims for Patent: 7,959,920

Summary for Patent: 7,959,920

Title:	Methods of making and using antibodies directed to parathyroid hormone
Abstract:	The present invention relates to methods of generating anti-PTH antibodies and antibodies generated by the methods of the invention. The anti-PTH antibodies generated by the methods of the invention specifically bind to an epitope on a whole PTH (1-84) which includes one or more N-terminal amino acid residues of the whole PTH. The anti-PTH antibodies generated are useful for detecting whole PTH in a biological sample and for diagnosing parathyroid diseases or disorders.
Inventor(s):	Cantor; Thomas L. (El Cajon, CA)
Assignee:	Scantibodies Laboratory, Inc. (Santee, CA)
Application Number:	11/516,912
Patent Claims:	1. A method of generating an antibody that specifically binds to a target parathyroid hormone (PTH) peptide, said method comprising: a) introducing a PTH peptide immunogen to a mammal in an amount sufficient to produce an antibody to said PTH peptide immunogen; wherein said PTH peptide immunogen has an amino acid sequence which starts at amino acid position 1 and ends at amino acid position 84 of a whole PTH; wherein the whole PTH has the amino acid sequence: (SEQ ID NO:1) TABLE-US-00017 Ser Val Ser Glu Ile Gln Leu Met His Asn Leu 1 5 10 Gly Lys His Leu Asn Ser Met Glu Arg Val Glu 15 20 Trp Leu Arg Lys Lys Leu Gln Asp Val His Asn 25 30 Phe Val Ala Leu Gly Ala Pro Leu Ala Pro Arg 35 40 Asp Ala Gly Ser Gln Arg Pro Arg Lys Lys Glu 45 50 55 Asp Asn Val Leu Val Glu Ser His Glu Lys Ser 60 65 Leu Gly Glu Ala Asn Lys Ala Asp Val Asn Val 70 75 Leu Thr Lys Ala Lys Ser Gln; 80 b) recovering said antibody from said mammal; c) purifying said antibody by a combination of a positive adsorption to a PTH peptide consisting of an amino acid sequence consisting of PTH.sub.1-34, and a negative adsorption to a PTH peptide having an amino acid sequence which starts at amino acid position 2 and ends at amino acid position 34 of said whole PTH. 2. The method of claim 1, wherein the positive adsorption to a PTH peptide in step c) is performed before the negative adsorption to a PTH peptide in step c). 3. The method of claim 1, wherein the positive adsorption to a PTH peptide in step c) is performed after the negative adsorption to a PTH peptide in step c).

Details for Patent 7,959,920

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Nps Pharmaceuticals, Inc.	NATPARA	parathyroid hormone	For Injection	125511	01/23/2015		2025-09-06