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Last Updated: April 26, 2024

Claims for Patent: 7,932,087

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Summary for Patent: 7,932,087

Title:	Method of expressing recombinant protein in CHO cells
Abstract:	Method of expressing recombinant protein in CHO cells, by using an expression vector comprising the murine IgG 2A gene locus.
Inventor(s):	Kallmeier; Robert (Holmer Green, GB), Gay; Robert (Pinner Middx, GB)
Assignee:	Lonza Biologics PLC (Berkshire, GB)
Application Number:	11/962,261
Patent Claims:	1. The method for enhancing the transfection rate of a mammalian expression vector in CHO cells by transfecting the cells with an expression vector that comprises a transcription unit for a product gene which transcription unit is under control of the mCMV promoter, said vector further comprising a portion from the murine IgG 2A gene locus which portion is enhancing the activity of the mCMV promoter. 2. The method according to claim 1 wherein the expression vector further comprises a second transcription unit comprising a glutamine synthetase (GS) marker gene. 3. The method according to claim 1, characterized in that the mCMV promoter comprises the natural transcription start site (+0) and extends upstream to position -500. 4. The method according to claim 3, characterized in that the mCMV promoter extends to the natural Xho I restriction site. 5. The method according to claim 1, characterized in that the mCMV promoter lacks the first, natural intron of the mCMV promoter.

Details for Patent 7,932,087

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Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986	⤷ Try a Trial	2022-07-19
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132		⤷ Try a Trial	2022-07-19
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132		⤷ Try a Trial	2022-07-19
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

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