Claims for Patent: 7,626,077

Summary for Patent: 7,626,077

Title:	Glyphosate-resistant plants
Abstract:	This invention relates to glyphosate-resistant transgenic plants and methods of making the same. In a preferred embodiment, a DNA fragment which comprises an EPSPS 5\' regulatory sequence and a glyphosate-resistant EPSPS coding sequence is introduced into regenerable plant cells. The encoded EPSPS has a chloroplast transit peptide. The DNA fragment does not contain a non-EPSPS enhancer. Cells are selected for stable transformation, and the selected cells can be used to regenerate glyphosate-resistant transgenic plants. The DNA fragment used for transformation preferably comprises a modified plant genomic sequence, such as SEQ ID NO: 2, SEQ ID NO:4 or SEQ ID NO: 6. In one embodiment, two DNA fragments of this invention are stably transformed into a plant to confer glyphosate-resistance.
Inventor(s):	Held; Bruce M. (Ames, IA), Wilson; Herbert M. (Ames, IA), Dykema; Philip E. (Ames, IA), Lewnau; Carol J. (Ames, IA), Eby; Janelle C. (Ames, IA)
Assignee:	Mertec LLC (West Point, IA)
Application Number:	11/409,909
Patent Claims:	1. A method of making a glyphosate-resistant plant, comprising: (a) introducing a DNA fragment into a plurality of regenerable plant cells; (b) selecting from said plurality of plant cells at lease one glyphosate-resistant plant cell; and (c) using the selected at least one glyphosate-resistant plant cell to regenerate glyphosate-resistant plants; wherein the DNA fragment comprises a non-heterologous EPSPS 5' regulatory sequence, a coding sequence encoding a glyphosate-resistant EPSPS, wherein the EPSPS 5' regulatory sequence is operably linked to the coding sequence, intron and exon sequences of an EPSPS genomic sequence, and a non-heterologous EPSPS 3' regulatory sequence, and wherein the DNA fragment further comprises a non-heterologous chloroplast transit peptide but does not contain a non-EPSPS enhancer; such that when said plant is exposed to glyphosate at commercial rates, said plant is resistant to said glyphosate. 2. The method according to claim 1, wherein the coding sequence consists of a plant genomic sequence with at least two nucleotide mutations, and wherein the plant genomic sequence with at least two nucleotide mutations encodes a glyphosate-resistant EPSPS. 3. The method according to claim 1, wherein the glyphosate-resistant plant made by the method of claim 1 is soybean. 4. The method according to claim 1, wherein the glyphosate-resistant plant made by the method of claim 1 is corn. 5. The method according to claim 1, wherein the EPSPS 5' regulatory sequence comprises at least 400 bp. 6. The method according to claim 1, wherein the EPSPS 5' regulatory sequence comprises at least 1000 bp. 7. The method according to claim 1, wherein the EPSPS 5' regulatory sequence is an Arabidopsis thaliana genomic sequence and comprises at least 1,200 bp. 8. The method according to claim 1, wherein the EPSPS 5' regulatory sequence is a Zea mays genomic sequence and comprises at least 1,800 bp. 9. A method of making a glyphosate-resistant plant, comprising: (a) introducing a DNA fragment into a plurality of regenerable plant cells; (b) selecting from said plurality of plant cells at lease one glyphosate-resistant plant cell; and (c) using the selected at least one glyphosate-resistant plant cell to regenerate glyphosate-resistant plants; wherein the DNA fragment is derived from Zea mays or Arabidopsis thaliana and comprises a non-heterologous EPSPS 5' regulatory sequence and a coding sequence encoding a glyphosate-resistant EPSPS, wherein the EPSPS 5' regulatory sequence is operably linked to the coding sequence, intron and exon sequences of a EPSPS genomic sequence, a non-heterologous plant EPSPS 3' regulatory sequence, and wherein the DNA fragment further comprises a non-heterologous chloroplast transit peptide but does not contain a non-EPSPS enhancer; such that when said plant is exposed to glyphosate at commercial rates, said plant is resistant to said glyphosate. 10. The method according to claim 9, wherein the coding sequence consists of a plant genomic sequence with at least two nucleotide substitutions, and wherein the plant genomic sequence with at least two nucleotide substitutions encodes a glyphosate-resistant EPSPS. 11. A method of making a glyphosate-resistant plant cell, comprising: (a) isolating a nucleic acid having a sequence encoding an endogenous genomic EPSPS gene from a plant cell, wherein said sequence comprises the genomic EPSPS gene of said plant cell, comprising a complete non-heterologous EPSPS 5' regulatory sequence, 3' EPSPS regulatory sequence, and intron and exon sequences of said EPSPS gene; (b) mutating said nucleic acid such that it encodes a glyphosate-resistant EPSPS; (c) introducing said nucleic acid into a plant cell, wherein said nucleic acid does not contain a non-EPSPS enhancer; and (d) producing a plant from the plant cell, such that the plant is glyphosate resistant when exposed to said glyphosate at commercial rates.

Details for Patent 7,626,077

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2022-08-19
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2022-08-19
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2022-08-19