Claims for Patent: 7,585,837

Summary for Patent: 7,585,837

Title:	Reversible pegylated drugs
Abstract:	Reversible pegylated drugs are provided by derivatization of free functional groups of the drug selected from amino, hydroxyl, mercapto, phosphate and/or carboxyl with groups sensitive to mild basic conditions such as 9-fluorenylmethoxycarbonyl (Fmoc) or 2-sulfo-9-fluorenylmethoxycarbonyl (FMS), to which group a PEG moiety is attached. In these pegylated drugs, the PEG moiety and the drug residue are not linked directly to each other, but rather both residues are linked to different positions of the scaffold Fmoc or FMS structure that is highly sensitive to bases and is removable under physiological conditions. The drugs are preferably drugs containing an amino group, most preferably peptides and proteins of low or medium molecular weight. Similar molecules are provided wherein a protein carrier or another polymer carrier replaces the PEG moiety.
Inventor(s):	Shechter; Yoram (Rehovot, IL), Fridkin; Matityahu (Rehovot, IL), Tsubery; Haim (Elad, IL)
Assignee:	Yeda Research and Development Co. Ltd. (Rehovot, IL)
Application Number:	11/244,402
Patent Claims:	1. A compound of the formula: (X).sub.n--Y wherein Y is a moiety of a drug bearing at least one functional group selected from free amino, carboxyl, phosphate, hydroxyl and/or mercapto, and X is a radical of the formula (i): ##STR00045## wherein: R.sub.1 is a radical containing a protein or a polymer carrier moiety wherein said polymer carrier has suitable functional groups and is selected from the group consisting of linear or branched polyethylene glycol (PEG) having a molecular weight in the range of 5000 to 80000 Da; copolymers of linear or branched PEG; poly(lactic acid) or copolymers thereof; polyesters having suitable functional groups based on polylactide (PLA), polyglycolide (PGA), polycaprolactone (PCL), or their copolymers; and polyamides based on polymethacrylamide or their copolymers; R.sub.2 is selected from the group consisting of hydrogen, (C1-C8)alkyl, (C1-C8) alkoxy, (C1-C8)alkoxyalkyl, (C6-C10)aryl, (C1-C8)alkaryl, (C6-C10)ar(C1-C8)alkyl, halogen, nitro, --SO.sub.3H, --SO.sub.2NHR, amino, ammonium, carboxyl, PO.sub.3H.sub.2, and OPO.sub.3H.sub.2, and R is selected from the group consisting of hydrogen, (C1-C8)alkyl and (C6-C10)aryl; R.sub.3 and R.sub.4, the same or different, are each selected from the group consisting of hydrogen, (C.sub.1-C.sub.8)alkyl and (C10-C10)aryl; A is a covalent bond when the radical is linked to a carboxyl, phosphate or mercapto group of the drug Y, or A is OCO when the radical is linked to an amino or hydroxyl group of the drug Y; n is an integer of at least one, or pharmaceutically acceptable salts thereof. 2. A compound according to claim 1 wherein said protein carrier is selected from the group consisting of albumin, a modified albumin, and a protein containing globin-like domains having long half-life in circulation. 3. A compound according to claim 2 wherein said albumin is human serum albumin (HSA), said modified albumin is cationized bovine serum albumin (CBSA) or cationized human serum albumin (CHSA), and said protein containing globin-like domains having long half-life in circulation is hemoglobin A or S. 4. A compound according to claim 3 wherein said drug Y is insulin. 5. The compound according to claim 4 wherein the protein is human serum albumin linked via a spacer to the radical (i) wherein R.sub.2, R.sub.3 and R.sub.4 each is H and A is OCO--, herein designated HAS-Fmoc-Insulin. 6. A compound according to claim 1 wherein said polymer carrier is poly(lactic acid)-block-polyethylene glycol, N-(2-hydroxypropyl)methacrylamide (HMPA) copolymer or poly-D,L-lactide-co-glycolide (PLGA) nanoparticles. 7. A compound according to claim 1 wherein the functional groups of said polymer carrier are selected from the group consisting of hydroxy, mercapto, amino, carboxyl, sulfonic acid group, and combinations thereof. 8. A compound according to claim 1 wherein said polymer carrier is liposomes containing phospholipids with covalently attached poly(ethylene glycol). 9. A compound according to claim 1 wherein said polymer carrier is in the form of nanoparticles. 10. A compound according to claim 1 wherein the polymer carrier is linear or branched PEG. 11. A compound according to claim 10 wherein in formula (I) R.sub.2, R.sub.3 and R.sub.4 each is hydrogen and A is --OCO--(hereinafter "Fmoc"). 12. A compound according to claim 10 wherein in formula (I) R.sub.2 is --SO.sub.3H at position 2 of the fluorene ring, R.sub.3 and R.sub.4 each is hydrogen, and A is --OCO-- (hereinafter "FMS"). 13. A compound according to claim 10 wherein R.sub.1 is a radical of the formula: --R.sub.5--R.sub.6-PEG wherein R.sub.5 is selected from the group consisting of --NH--, --S--, --CO--, --COO--, --CH.sub.2--, --SO.sub.2--, --SO.sub.3--, --PO.sub.2--, and --PO.sub.3--, and R.sub.6 is a bond or a radical through which the polyethylene glycol (PEG) moiety is covalently attached to R.sub.5. 14. A compound according to claim 13 wherein: R.sub.5 is --NH--; R.sub.6 is selected from the group consisting of --CO--, --COO--, --CH.sub.2--, --CH(CH.sub.3)--, CO--NH--, --CS--NH, --CO--CH.sub.2--NH--CO--, --CO-- CH(CH.sub.3)--NH--CO--, --CO--CH.sub.2--NH--CO--NH, ##STR00046## Z is O, S or NH that is linked to the PEG moiety; and R.sub.7 is selected from the group consisting of C1-C18 straight or branched alkylene, phenylene, an oxyalkylene radical having 3-18 carbon atoms in the backbone, a residue of a peptide containing 2-10 amino acid residues, and a residue of a saccharide containing 1-10 monosaccharide residues. 15. A compound according to claim 10 wherein the PEG moiety has a molecular weight in the range of 5000 to 40000 Da. 16. A compound according to claim 15 wherein the PEG moiety is a branched molecule of 40000 Da. 17. A compound according to claim 15 wherein the PEG moiety is a branched molecule of 5000 Da. 18. A compound according to claim 14 wherein Y is a moiety of a drug containing at least one amino group. 19. A compound according to claim 18 wherein said drug is an antibiotic aminoglycoside or an antineoplastic drug. 20. A compound according to claim 19 wherein said antibiotic aminoglycoside is gentamicin or amphotericin and said antineoplastic drug is aminolevulinic acid, daunorubicin or doxorubicin. 21. A compound according to claim 18, wherein said drug is a peptide or a protein drug of low or medium molecular weight. 22. A compound according to claim 21 wherein said peptide or protein is selected from the group consisting of insulin, an interferon, a PYY agonist, an exendin, an exendin analogue or exendin agonist, atrial natriuretic peptide (ANP), human growth hormone (hGH), erythropoietin, TNF-.alpha., calcitonin, gonadotropin releasing hormone (GnRH), a GnRH analogue, hirudin, glucagon, and a monoclonal antibody fragment. 23. A compound according to claim 22 wherein said interferon is IFN-.alpha.2, said PYY agonist is the peptide PYY.sub.3-36, said exendin is exendin-3 or exendin-4, and said monoclonal antibody fragment is anti-TNF-.alpha. monoclonal antibody fragment. 24. A compound according to claim 14 of the formula: ##STR00047## wherein R.sub.2 is H or --SO.sub.3H at position 2 of the fluorene ring, Y is the residue of a drug containing at least one amino group, and PEG is a linear or branched PEG moiety of molecular weight in the range of 5000 to 80000 Da. 25. A compound according to claim 24 wherein the PEG moiety has a molecular weight in the range of 5000 to 40000 Da. 26. A compound according to claim 25 wherein the PEG moiety is a branched molecule of 40000 Da. 27. A compound according to claim 25 wherein the PEG moiety is a branched molecule of 5000 Da. 28. A compound according to claim 24 wherein R.sub.2 is H, herein designated PEG-Fmoc-drug conjugate. 29. A compound according to claim 28 wherein the conjugate has the formula (PEG-Fmoc).sub.n-drug, wherein n is an integer from 1 to 3. 30. A compound according to claim 29 wherein the PEG moiety has a molecular weight in the range of 5000 to 40000 Da. 31. A compound according to claim 30 wherein the PEG moiety is a branched molecule of 40000 Da. 32. A compound according to claim 30 wherein the PEG moiety is a branched molecule of 5000 Da. 33. A compound according to claim 29 wherein the drug is an antibiotic aminoglycoside drug or an antineoplastic drug. 34. A compound according to claim 33 wherein said antibiotic aminoglycoside drug is gentamicin or amphotericin B, and said antineoplastic drug is aminolevulinic acid, daunorubicin or doxorubicin. 35. A compound according to claim 29 wherein the drug is a peptide or a protein drug of low or medium molecular weight. 36. A compound according to claim 35 wherein said peptide or protein is selected from the group consisting of insulin, an interferon, a PYY agonist, an exendin, an exendin analogue, an exendin agonist, atrial natriuretic peptide (ANP), human growth hormone (hGH), erythropoietin, TNF-.alpha., calcitonin, gonadotropin releasing hormone (GnRH), a GnRH analogue, hirudin, glucagon, and a monoclonal antibody fragment. 37. A compound according to claim 36 wherein said interferon is IFN-.alpha.2, said PYY agonist is the peptide PYY.sub.3-36, said exendin is exendin-3 or exendin-4, and said monoclonal antibody fragment is anti-TNF-.alpha. monoclonal antibody fragment. 38. A compound according to claim 24 wherein R.sub.2 is --SO.sub.3H, herein designated PEG-FMS-drug conjugate. 39. A compound according to claim 38 wherein the conjugate has the formula (PEG-FMS).sub.n-drug, wherein n is an integer from 1 to 3. 40. A compound according to claim 39 wherein the PEG moiety has a molecular weight in the range of 5000 to 40000 Da. 41. A compound according to claim 40 wherein the PEG moiety is a branched molecule of 40000 Da. 42. A compound according to claim 41 wherein the PEG moiety is a branched molecule of 5000 Da. 43. A compound according to claim 39 wherein the drug is an antibiotic aminoglycoside drug or an antineoplastic drug. 44. A compound according to claim 43 wherein said antibiotic aminoglycoside drug is gentamicin or amphotericin B, and said antineoplastic drug is aminolevulinic acid, daunorubicin or doxorubicin. 45. A compound according to claim 39 wherein the drug is a peptide or a protein drug of low or medium molecular weight. 46. A compound according to claim 45 wherein said peptide or protein is selected from the group consisting of insulin, an interferon, a PYY agonist, an exendin, an exendin analogue, an exendin agonist, atrial natriuretic peptide (ANP), human growth hormone (hGH), erythropoietin, TNF-.alpha., calcitonin, gonadotropin releasing hormone (GnRH), a GnRH analogue, hirudin, glucagon, and a monoclonal antibody fragment. 47. A compound according to claim 46 wherein said interferon is IFN-.alpha.2, said PYY agonist is the peptide PYY.sub.3-36, said exendin is exendin-3 or exendin-4, and said monoclonal antibody fragment is anti-TNF-.alpha. monoclonal antibody fragment. 48. A compound according to claim 45 wherein said peptide or protein drug is insulin. 49. A compound according to claim 48 wherein said insulin is human insulin. 50. The compound according to claim 49 wherein said compound is herein identified as the conjugate PEG.sub.40-FMS-insulin. 51. A compound according to claim 46 wherein said peptide or protein drug is an interferon. 52. A compound according to claim 51 wherein said interferon is IFN-.alpha.2. 53. The compound according to claim 52 wherein said compound is herein identified as the conjugate PEG.sub.40-FMS-IFN-.alpha.2 or (PEG.sub.40-FMS).sub.2-IFN-.alpha.2. 54. A compound according to claim 46 wherein said peptide or protein drug is a PYY agonist. 55. The compound according to claim 54 wherein said PYY agonist is PYY.sub.3-36 [SEQ ID NO:12]. 56. The compound according to claim 55 herein identified as the conjugate PEG.sub.40-PMS-PYY.sub.3-36 (PEG.sub.40-FMS-[SEQ ID NO:12]). 57. A compound according to claim 46 wherein said peptide or protein drug is human growth hormone (hGH). 58. The compound according to claim 57 herein identified as the conjugate PEG.sub.40-FMS-hGH or (PEG.sub.40-FMS).sub.2-hGH. 59. A compound according to claim 46 wherein said peptide or protein drug is atrial natriuretic peptide (ANP) [SEQ ID NO:13]. 60. The compound according to claim 59 herein identified as the conjugate PEG.sub.40-FMS-ANP. 61. A compound according to claim 46 wherein said peptide or protein drug is an exendin or an exendin agonist. 62. A compound according to claim 61 wherein said exendin is exendin-4 [SEQ ID NO:1]. 63. The compound according to claim 62 herein identified as the conjugate PEG.sub.40-FMS-exendin-4 (PEG.sub.40-FMS-[SEQ ID NO:1]). 64. A compound according to claim 61 wherein said exendin is exendin-3 [SEQ ID NO:2] or said exendin agonist is selected from the group consisting of [SEQ ID NO:3] [SEQ ID NO:4], [SEQ ID NO:5], [SEQ ID NO:6], [SEQ ID NO:7], [SEQ ID NO:6], [SEQ ID NO:9], and [SEQ ID NO:10]. 65. A compound according to claim 14 of the formula: ##STR00048## wherein R.sub.2 is H or --SO.sub.3H at position 2 of the fluorene ring, Y is the residue of a drug containing at least one amino group, and PEG is a linear or branched PEG moiety of molecular weight in the range of 5000 to 80000 Da. 66. A compound according to claim 65 wherein the PEG moiety has a molecular weight in the range of 5000 to 40000 Da. 67. A compound according to claim 66 wherein the PEG moiety is a branched molecule of 40000 Da. 68. A compound according to claim 66 wherein the PEG moiety is a branched molecule of 5000 Da. 69. A compound according to claim 65 wherein Y is a moiety of a drug containing at least one amino group. 70. A compound according to claim 69 wherein said drug is an antibiotic aminoglycoside or an antineoplastic drug. 71. A compound according to claim 70 wherein said antibiotic aminoglycoside is gentamicin or amphotericin B, and said antineoplastic drug is aminolevulinic acid, daunorubicin or doxorubicin. 72. A compound according to claim 65, wherein Y is a moiety of a peptide or a protein drug of low or medium molecular weight. 73. A compound according to claim 72 wherein said peptide or protein is selected from the group consisting of insulin, an interferon, a PYY agonist, an exendin, an exendin analogue, an exendin agonist, atrial natriuretic peptide (ANP), human growth hormone (hGH), erythropoietin, TNF-.alpha., calcitonin, gonadotropin releasing hormone (GnRH), a GnRH analogue, hirudin, glucagon, and a monoclonal antibody fragment. 74. A compound according to claim 73 wherein said interferon is IFN-.alpha.2, said PYY agonist is the peptide PYY.sub.3-36, said exendin is exendin-3 or exendin-4, and said monoclonal antibody fragment is anti-TNF-.alpha. monoclonal antibody fragment. 75. A compound according to claim 13 wherein: R.sub.5 is --S--; and R.sub.6 is selected from the group consisting of ##STR00049## wherein Z is O, S or NH. 76. A compound according to claim 13 wherein: R.sub.5 is --CO; R.sub.6 is selected from the group consisting of O--; --NH--; --NH--R.sub.7--COO--; --NH--R.sub.7--NH; --NH--R.sub.7--CO--NH; ##STR00050## Z is O, S or NH; and R.sub.7 is selected from the group consisting of C1-C18 straight or branched alkylene, phenylene, an oxyalkylene radical having 3-18 carbon atoms in the backbone, a residue of a peptide containing 2-10 amino acid residues, and a residue of a saccharide containing 1-10 monosaccharide residues. 77. A compound according to claim 13 wherein: R.sub.5 is --CH.sub.2--; and R.sub.6 is --(CH.sub.2).sub.n--S-- or --(CH.sub.2).sub.n--NH--, wherein n is 0 to 18. 78. A compound according to claim 13 wherein: R.sub.5 is --SO.sub.2--; and R.sub.6 is O; --NH-- or --CH.sub.2--CH.sub.2--S. 79. A compound according to claim 13 wherein: R.sub.5 is --PO.sub.2--; and R.sub.6 is --O-- or --NH--. 80. A compound according to claim 75 wherein the radical R.sub.1 contains a linear or branched PEG moiety of molecular weight in the range of 5000 to 80000 Da. 81. A compound according to claim 80 wherein the PEG moiety has a molecular weight in the range of 5000 to 40000 Da. 82. A compound according to claim 81 wherein the PEG moiety is a branched molecule of 40000 Da or 5000 Da. 83. A compound according to claim 80 wherein Y is a moiety of a drug containing at least one amino group, said drug being selected from the group consisting of an antibiotic aminoglycoside, an antineoplastic drug, and a peptide or a protein drug of low or medium molecular weight wherein said peptide or protein is selected from the group consisting of insulin, an interferon, a PYY agonist, an exendin, an exendin analogue, an exendin agonist, atrial natriuretic peptide (ANP), human growth hormone (hGH), erythropoietin, TNF-.alpha., calcitonin, gonadotropin releasing hormone (GnRH), a GnRH analogue, hirudin, glucagon, and a monoclonal antibody fragment. 84. A pharmaceutical composition comprising a compound according to claim 1 or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier. 85. A pharmaceutical composition according to claim 84 comprising a compound wherein R.sub.1 is a protein carrier. 86. A pharmaceutical composition according to claim 85 comprising the compound herein designated HSA-Fmoc-Insulin. 87. A pharmaceutical composition according to claim 84 comprising a compound wherein R.sub.1 is polymer carrier. 88. A pharmaceutical composition according to claim 87 comprising a compound wherein R.sub.1 is linear or branched PEG. 89. A pharmaceutical composition according to claim 88 comprising a compound wherein R.sub.1 is a radical of the formula: --R.sub.5--R.sub.6-PEG wherein R.sub.5 is selected from the group consisting of --NH--, --S--, --CO--, --COO--, --CH.sub.2--, --SO.sub.2--, --SO.sub.3--, --PO.sub.2--, and --PO.sub.3--, and R.sub.6 is a bond or a radical through which the polyethylene glycol (PEG) moiety is covalently attached to R.sub.5. 90. A pharmaceutical composition according to claim 89 comprising a compound wherein R.sub.5 is --NH--; R.sub.6 is selected from the group consisting of --CO--, --COO--, --CH.sub.2--, --CH(CH.sub.3)--, CO--NH--, --CS--NH, --CO--CH.sub.2--NH--CO--, --CO-- CH(CH.sub.3)--NH--CO--, --CO--CH.sub.2--NH--CO--NH, ##STR00051## Z is O, S or NH that is linked to the PEG moiety; and R.sub.7 is selected from the group consisting of C1-C18 straight or branched alkylene, phenylene, an oxyalkylene radical having 3-18 carbon atoms in the backbone, a residue of a peptide containing 2-10 amino acid residues, and a residue of a saccharide containing 1-10 monosaccharide residues. 91. A pharmaceutical composition according to claim 90 comprising a compound of the formula: ##STR00052## wherein R.sub.2 is H or --SO.sub.3H at position 2 of the fluorene ring, Y is the residue of a drug containing at least one amino group, and PEG is a linear or branched PEG moiety of molecular weight in the range of 5000 to 80000 Da. 92. A pharmaceutical composition according to claim 91 wherein the PEG moiety is a branched molecule of 40000 Da. 93. A pharmaceutical composition according to claim 92 wherein R.sub.2 is --SO.sub.3H, herein designated (PEG-FMS).sub.n-drug, wherein n is an integer from 1 to 3. 94. A pharmaceutical composition according to claim 93 wherein the drug is an antibiotic aminoglycoside drug or an antineoplastic drug. 95. A pharmaceutical composition according to claim 93 wherein the drug is a peptide or a protein drug of low or medium molecular weight. 96. A pharmaceutical composition according to claim 95 wherein said peptide or protein is selected from the group consisting of insulin, an interferon, a PYY agonist, an exendin, an exendin analogue, an exendin agonist, atrial natriuretic peptide (ANP), human growth hormone (hGH), erythropoietin, TNF-.alpha., calcitonin, gonadotropin releasing hormone (GnRH), a GnRH analogue, hirudin, glucagon, and a monoclonal antibody fragment. 97. A pharmaceutical composition according to claim 96 comprising a pegylated PMS conjugate of insulin. 98. A pharmaceutical composition according to claim 96 comprising a pegylated FMS conjugate of IFN-.alpha.2. 99. A pharmaceutical composition according to claim 96 comprising a pegylated FMS conjugate of PYY.sub.3-36 (SEQ ID NO:12). 100. A pharmaceutical composition according to claim 96 comprising a pegylated FMS conjugate of hGH. 101. A pharmaceutical composition according to claim 96 comprising a pegylated FMS conjugate of ANP. 102. A pharmaceutical composition according to claim 96 comprising a pegylated FMS conjugate of an exendin or exendin agonist. 103. A pharmaceutical composition according to claim 102 comprising the pegylated FMS conjugate of exendin-4 agonist PEG.sub.40-FMS-exendin-4 (PEG.sub.40-FMS-[SEQ ID NO:1]). 104. A compound according to claim 29 wherein n is 1 or 2. 105. A compound according to claim 39 wherein n is 1 or 2.

Details for Patent 7,585,837

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Eli Lilly And Company	HUMULIN R U-100	insulin human	Injection	018780	10/28/1982		2023-04-08
Eli Lilly And Company	HUMULIN R U-500	insulin human	Injection	018780	12/29/2015		2023-04-08
Eli Lilly And Company	HUMULIN R U-100	insulin human	Injection	018780	08/06/1998		2023-04-08
Eli Lilly And Company	HUMULIN R U-500	insulin human	Injection	018780	03/31/1994		2023-04-08
Eli Lilly And Company	HUMULIN R U-100	insulin human	Injection	018780	05/25/2018		2023-04-08
Grifols Therapeutics Llc	ALBUKED, PLASBUMIN-20, PLASBUMIN-25, PLASBUMIN-5	albumin (human)	For Injection	101138	10/21/1942		2023-04-08