Claims for Patent: 7,507,547

Summary for Patent: 7,507,547

Title:	Screening assays for antioxidants and antiproliferative compounds
Abstract:	Provided are screening assays for identifying and evaluating compounds with antioxidant and/or antiproliferative activities.
Inventor(s):	Carraway; Robert E. (Boston, MA)
Assignee:	University of Massachusetts (Boston, MA)
Application Number:	11/223,395
Patent Claims:	1. A method of identifying a candidate compound with antioxidant or antiproliferative activity, or both, the method comprising: contacting a test compound to a cell expressing neurotensin receptors on its cell surface; contacting the cell with a neurotensin receptor ligand; monitoring binding of the ligand to the cell; and selecting the test compound as a candidate compound with antioxidant or antiproliferative activity if the test compound enhances binding of the ligand to the cell, relative to binding of the ligand to a cell of the same cell type that is not contacted with the test compound. 2. The method of claim 1, wherein the test compound is a compound that belongs to a class selected from the group consisting of: dihydropyridines, polyphenols, flavonoids, isoprenoids, retinoids, inhibitors of mitochondrial function, inhibitors of glycolysis, inhibitors of glycogen synthase kinase, inhibitors of flavoprotein oxidases, iron/zinc chelators, inhibitors of lipoxygenases, inhibitors of protein kinase C, inhibitors of PI3-kinase, inhibitors of tyrosine kinases, and estrogen agonist. 3. The method of claim 1, wherein the ligand is neurotensin. 4. The method of claim 1, wherein the ligand is selected from the group consisting of neurotensin; a neurotensin fragment comprising neurotensin (8-13); neurotensin or an analog or fragment thereof with a substitution at one or more of the following positions: Arg 8, Arg 9, Pro 10, or Tyr 11, Ile 12, or Leu 13; a neurotensin with tryptophan substitution for Tyr 11; a neurotensin analog or fragment thereof MP-2530; Neuromedin-N; xenopsin; xenin; histamine releasing peptide; SR48692; SR142948A; and levocabastine. 5. The method of claim 1, wherein the ligand is radiolabeled. 6. The method of claim 1, wherein the cell is a cultured cell from a tumor selected from the group consisting of a Ewing's sarcoma, a myeloma, an astrocytoma, a lung tumor, a colon tumor, an ovarian tumor, a pancreatic tumor, and a prostate tumor. 7. The method of claim 1, further comprising: contacting a cancer cell with the selected candidate compound; monitoring proliferation of the cancer cell; and identifying the candidate compound as an antiproliferative agent if the candidate compound inhibits proliferation of the cancer cell relative to proliferation of a cancer cell of the same cell type that is not contacted with the candidate compound. 8. The method of claim 7, wherein the cancer cell is a cell selected from the group consisting of a Ewing's sarcoma cell, a myeloma cell, an astrocytoma cell, a lung tumor cell, a colon tumor cell, an ovarian tumor cell, a pancreatic tumor cell, and a prostate tumor cell. 9. The method of claim 1, wherein the method is repeated for a plurality of test compounds. 10. The method of claim 1, wherein the cell is selected from the group consisting of a Ewing's sarcoma cell, a myeloma cell, an astrocytoma cell, a lung tumor cell, a colon tumor cell, an ovarian tumor cell, a pancreatic tumor cell, and a prostate tumor cell. 11. The method of claim 1, wherein the test compound is selected as a candidate compound with antiproliferative activity if the test compound enhances binding of the ligand to the cell, relative to binding of the ligand to a cell of the same cell type that is not contacted with the test compound. 12. The method of claim 11, wherein the test compound is a compound that belongs to a class selected from the group consisting of: dihydropyridines, polyphenols, flavonoids, isoprenoids, retinoids, inhibitors of mitochondrial function, inhibitors of glycolysis, inhibitors of glycogen synthase kinase, inhibitors of flavoprotein oxidases, iron/zinc chelators, inhibitors of lipoxygenases, inhibitors of protein kinase C, inhibitors of PI3-kinase, inhibitors of tyrosine kinases, and estrogen agonist. 13. The method of claim 11, wherein the ligand is neurotensin. 14. The method of claim 11, wherein the ligand is selected from the group consisting of neurotensin; a neurotensin fragment comprising neurotensin (8-13); neurotensin or an analog or fragment thereof with a substitution at one or more of the following positions: Arg 8, Arg 9, Pro 10, or Tyr 11, Ile 12, or Leu 13; a neurotensin with tryptophan substitution for Tyr 11; a neurotensin analog or fragment thereof MP-2530; Neuromedin-N; xenopsin; xenin; histamine releasing peptide; SR48692; SR142948A; and levocabastine. 15. The method of claim 11, wherein the ligand is radiolabeled. 16. The method of claim 11, wherein the cell is a cultured cell from a tumor selected from the group consisting of a Ewing's sarcoma, a myeloma, an astrocytoma, a lung tumor, a colon tumor, an ovarian tumor, a pancreatic tumor, and a prostate tumor.

Details for Patent 7,507,547

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Jubilant Hollisterstier Llc	N/A	positive skin test control-histamine	Injection	103891	03/13/1924		2024-09-09