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Last Updated: April 27, 2024

Claims for Patent: 6,767,729

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Summary for Patent: 6,767,729

Title:	Enzyme liquor and process for producing the same enzyme preparation protease preparations and protease-producing bacterium
Abstract:	An enzyme solution obtained by cultivating Bacillus subtilis M2-4 strain highly heat-resistant peptidase activity such that the residual activity thereof after 1-hr heat treatment at 60 to 65.degree. C. at pH 7 is substantially 100%. An enzyme preparation can be obtained by separating the enzyme protein from the enzyme solution. Using such enzyme solution or enzyme preparation as an active component, a proteolytic enzyme preparation can be prepared. The enzyme solution, the enzyme preparation and the proteolytic enzyme preparation have both high heat-resistance and great potency of hydrolyzing proteins into low molecules.
Inventor(s):	Nagano; Hiroko (Aichi, JP), Shoji; Zenya (Akita, JP), Hirano; Kenichi (Gifu, JP), Ando; Keiichi (Gifu, JP)
Assignee:	Amano Enzyme Inc. (Nagoya, JP)
Application Number:	09/926,623
Patent Claims:	1. A method for producing an isolated peptidase solution, comprising cultivating Bacillus subtilis M2-4 strain (FERM BP-7155) to produce a culture, wherein said Bacillus subtilis M2-4 strain (FERM BP-7155) is isolated from dough of Mantou, and obtaining the peptidase solution from the culture, wherein said peptidase solution is highly heat resistant such that the residual activity thereof after 1-hr heat treatment at 60 to 65.degree. C. at pH 7 is substantially 100%. 2. The method of claim 1, wherein the peptidase solution also has protease and collagenase activity. 3. The method of claim 2, wherein said peptidase solution has aminopeptidase activity. 4. The method of claim 1, wherein said peptidase solution also has protease activity. 5. The method of claim 4, wherein said protease activity is neutral protease activity. 6. The method of claim 4, wherein said protease activity is alkaline protease activity. 7. The method of claim 1, wherein said peptidase solution also has collagenase activity.

Details for Patent 6,767,729

Glossary

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Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965	⤷ Try a Trial	2019-05-27
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

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