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Last Updated: January 29, 2022

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Claims for Patent: 6,750,378

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Summary for Patent: 6,750,378
Title: Maize H3C4 promoter combined with the first intron of rice actin, chimeric gene comprising it and transformed plant
Abstract:The present invention relates to a DNA sequence, a 5\' regulatory element allowing the expression of a heterologous gene in a plant cell from a monocotyledonous plant, characterized in that it comprises, in the direction of transcription, a first DNA sequence, which is a functional fragment of the sequence of the maize H3C4 promoter, and a second DNA sequence, which is a functional fragment of the sequence of the first intron of rice actin. The invention also relates to a chimeric gene comprising the said DNA sequence and the plants transformed with the said gene.
Inventor(s): Derose; Richard (Lyons, FR), Freyssinet; Georges (Cyr Au Mont d\'Or, FR)
Assignee: Rhone-Poulenc Agro (Lyons, FR)
Application Number:09/037,531
Patent Claims:1. An isolated DNA sequence comprising, in the direction of transcription, a fragment of the sequence of the maize H3C4 promoter wherein said fragment of the sequence of the maize H3C4 promoter has the sequence shown in SEQ ID NO: 1 and a fragment of the sequence of the first intron of rice actin wherein said fragment of the sequence of the first intran of rice actin has the sequence shown in SEQ ID NO: 2.

2. An isolated DNA sequence comprising the DNA sequence shown in SEQ ID NO: 3.

3. An expression cassette comprising a coding sequence and 5' and 3' heterologous regulatory elements, wherein the 5' regulatory element comprises the isolated DNA sequence according to claim 1, and wherein said expression cassette functions in monocotyledonous plants or plant cells.

4. An expression cassette according to claim 3, wherein the coding sequence is a DNA sequence encoding a protein of interest.

5. The expression cassette according to claim 4, wherein the DNA sequence encoding a protein of interest is a DNA sequence encoding a selectable marker.

6. The expression cassette according to claim 4, wherein the DNA sequence encoding a protein of interest is selected from the group consisting of a DNA sequence encoding a protein that confers herbicide tolerance, a DNA sequence encoding a protein that confers insect tolerance, and a DNA sequence encoding a protein that confers disease tolerance.

7. The expression cassette according to claim 6, wherein the DNA sequence encoding a protein that confers herbicide tolerance is selected from the group consisting of a DNA sequence encoding the Bar gene conferring tolerance to bialophos, a DNA sequence encoding an EPSPS conferring resistance to herbicides having EFSPS as a target, a DNA sequence encoding glyphosate oxidoreductase, and a DNA sequence encoding an HPPD conferring tolerance to herbicides having HPPD as a target.

8. The expression cassette according to claim 7, wherein the DNA sequence encoding a protein that confers herbicide tolerance is a DNA sequence encoding an EPSPS or a DNA sequence encoding an HPPD.

9. The expression cassette according to claim 8, wherein the DNA sequence encoding a protein that confers herbicide tolerance is a DNA sequence encoding an EPSPS.

10. A cloning or expression vector for the transformation of a plant cell or of a plant, which comprises the expression cassette according to claim 3 and at least one replication origin.

11. The cloning or expression vector according to claim 10, which is a plasmid.

12. A method of transforming plant cells, comprising integrating the expression cassette according to claim 3 into the plant cells resulting in transformed plant cells.

13. A plant cell which contains at least one expression cassette according to claim 3.

14. A transformed plant which comprises the plant cell according to claim 13.

15. A transformed plant which is regenerated from a plant cell according to claim 13.

16. A transformed plant produced from the culture of a transformed plant according to claim 14 or the crossing of a transformed plant according to claim 14 with another plant.

17. Seed of the transformed plant according to claim 14.

18. The expression cassette according to claim 9, wherein the DNA sequence encoding an EPSPS is CP4 or a double-mutant EPSPS.

19. A method of controlling weeds in a field comprising weeds and seeds or plants, said weeds or plants each comprising the expression cassette according to claim 7, which method consists in applying to the field a dose of herbicide which is toxic to the weeds but to which the seeds or plants are tolerant.

20. A method of cultivating plants transformed with the expression cassette according to claim 7, which method comprises: sowing seeds comprising said expression cassette in a field comprising weeds; cultivating plants from the seeds; applying to the field a dose of herbicide which is toxic to the weeds but to which the seeds or plants are tolerant; and harvesting the cultivated plants when they reach maturity.

21. The method according to claim 5, wherein the applying step is before the sowing step, or during or after the sowing step.

22. A transformed plant produced from the crossing of the transformed plant of claim 16.

Details for Patent 6,750,378

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 1986-06-04 ⤷  Try it Free 2017-12-14
Merck Sharp & Dohme Corp. INTRON A interferon alfa-2b For Injection 103132 ⤷  Try it Free 2017-12-14
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

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