Claims for Patent: 6,576,753

Summary for Patent: 6,576,753

Title:	DNA encoding human leukotriene C4 synthase
Abstract:	The present invention provides nucleotide sequences encoding human LTC.sub.4 synthase or variants thereof, which nucleotide sequences include a human LTC.sub.4 synthase genomic clone, and unique fragments thereof. The present invention also provides recombinant plasmids, and cells transformed therewith, including the LTC.sub.4 synthase gene sequences. The present invention further provides the chromosomal localization of the human LTC.sub.4 synthase gene, and also provides methods for identifying LTC.sub.4 synthase gene mutations that correlate with susceptibility to bronchial asthma. Diagnostic methods are also provided, as are methods for identifying LTC.sub.4 synthase modulators (i.e., compounds that alter the expression and/or activity of LTC.sub.4 synthase). The presents invention also provides functional equivalents of the LTC.sub.4 synthases, protein, and also methods of identifying LTC.sub.4 synthase inhibitors.
Inventor(s):	Lam; Bing K. (Roslindale, MA), Penrose; John F. (Norwood, MA), Austen; K. Frank (Wellesley, MA)
Assignee:	The Brigham and Women\'s Hospital, Inc. (Boston, MA)
Application Number:	09/809,592
Patent Claims:	1. An isolated human LTC.sub.4 synthase genomic clone consisting of SEQ ID NO.:10. 2. A unique fragment of LTC.sub.4 synthase genomic clone selected from the group consisting of: a fragment of intron 1, consisting of at least a portion of SEQ ID NO.:10 nucleotides 1505-2949; a fragment of intron 2, consisting of at least a portion of SEQ ID NO.:10 nucleotides 3050-3151; a fragment of intron 3, consisting of at least a portion of SEQ ID NO.:10 nucleotides 3223-3306; and a fragment of intron 4, consisting of at least a portion of SEQ ID NO.:10 nucleotides 3389-3618. 3. A unique fragment of human LCT4 synthase genomic clone consisting of at least a portion of nucleotides 1-1359 of SEQ ID NO.:10 upstream of the LTC.sub.4 start site. 4. An oligonucleotide primer specific for human LCT4 synthase genomic clone selected from the group consisting of: a fragment of intron 1, consisting of at least a portion of SEQ ID NO.:10 nucleotides 1505-2949; a fragment of intron 2, consisting of at least a portion of SEQ ID NO.:10 nucleotides 3050-3151; a fragment of intron 3, consisting of at least a portion of SEQ ID NO.:10 nucleotides 3223-3306; and a fragment of intron 4, consisting of at least a portion of SEQ ID NO.:10 nucleotides 3389-3618. 5. A hybridization probe specific for human LCT4 synthase genomic clone selected from the group consisting of: a fragment of intron 1, consisting of at least a portion of SEQ ID NO.:10 nucleotides 1505-2949; a fragment of intron 2, consisting of at least a portion of SEQ ID NO.:10 nucleotides 3050-3151; a fragment of intron 3, consisting of at least a portion of SEQ ID NO.:10 nucleotides 3223-3306; and a fragment of intron 4, consisting of at least a portion of SEQ ID NO.:10 nucleotides 3389-3618.

Details for Patent 6,576,753

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2014-05-20
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2014-05-20
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2014-05-20