Claims for Patent: 6,521,458

Summary for Patent: 6,521,458

Title:	Compositions and methods for improved plant transformation
Abstract:	The present invention provides methods for eliminating plants containing non-T-DNA sequences derived from a T-DNA vector. More specifically, the present invention provides a method for killing plant cells that receive non-T-DNA sequences based on incorporation of a lethal polynucleotide sequence into the non-T-DNA portion of the vector.
Inventor(s):	Gutterson; Neal (Oakland, CA), Hanson; William G. (Walnut Creek, CA)
Assignee:	DNA Plant Technology Corporation (Nogales, AZ)
Application Number:	09/302,980
Patent Claims:	1. A method for producing a transgenic plant containing a polynucleotide of interest, the method comprising: (a) introducing into a plurality of plant cells a T-DNA vector comprising: (i) a T-DNA sequence comprising a right border, a left border and the polynucleotide of interest positioned between the right border and left border, and (ii) a non-T-DNA sequence comprising a lethal polynucleotide sequence encoding a lethal polypeptide, wherein said non-T-DNA sequence is located beyond the left T-DNA border; (b) selecting a plant cell which comprises the T-DNA sequence and does not comprise the lethal polynucleotide sequence; and (c) regenerating a transgenic plant from the selected plant cell. 2. The method of claim 1, wherein the lethal polypeptide is a ribonuclease. 3. The method of claim 2, wherein the ribonuclease is Barnase. 4. The method of claim 3, wherein the Barnase has an intron in the coding region. 5. The method of claim 1, wherein the non-T-DNA sequence further comprises a screenable marker and the method further comprises detection of the screenable marker in the plant cells. 6. The method of claim 5, wherein the screenable marker encodes .beta.-glucuronidase. 7. The method of claim 1, wherein the lethal polynucleotide sequence is within about 5 kb of the left border. 8. An isolated T-DNA vector comprising a T-DNA sequence comprising a right border and a left border and a non-T-DNA sequence comprising a lethal polynucleotide sequence encoding a lethal polypeptide, wherein said non-T-DNA sequence is located beyond the left T-DNA border. 9. The isolated T-DNA vector of claim 8, further comprising a polynucleotide of interest positioned between the right border and left border of the T-DNA sequence. 10. The isolated T-DNA vector of claim 8, further comprising a selectable marker positioned between the right border and left border of the T-DNA sequence. 11. The isolated T-DNA vector claim 8, wherein the lethal polypeptide is a ribonuclease. 12. The isolated T-DNA vector claim 11, wherein the ribonuclease is Barnase. 13. The isolated T-DNA vector of claim 12, wherein the Barnase has an intron in the coding region. 14. The isolated T-DNA vector of claim 8, wherein the non-T-DNA sequence further comprises a screenable marker. 15. The isolated T-DNA vector of claim 14, wherein the screenabke marker encodes .beta.-glucuronidase. 16. The isolated T-DNA vector of claim 8, wherein the lethal polynucleotide sequence is within 5 kb of the left border.

Details for Patent 6,521,458

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2018-05-22
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2018-05-22
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2018-05-22