Claims for Patent: 6,429,193

Summary for Patent: 6,429,193

Title:	CELL CHEMOTACTIC FACTOR (CCTF) ORIGINATING IN MAMMALIAN TOOTH PRICEMENT OR CEMENT, METHOD FOR PURIFYING THE SAME, AND NOVEL CONNECTIVE TISSUE ADHESION PROMOTERS CONTAINING THE SAME AS THE ACTIVE INGREDIENT
Abstract:	A precementum- and/or cementum-derived chemotactic factor (CCTF) of a tooth of a Mammalian is characterized by a molecular weight measured by SDS-PAGE of 67000.+-.1000. A process for purifying a precementum- and/or cementum-derived gingival fibroblast chemotactic factor (CCTF) of a Mammalian tooth includes eluting a protein mixture from precementum and/or cementum of a Mammalian tooth and purifying the precementum- and/or cementum-derived chemotactic factor (CCTF) from the eluted protein mixture by molecular weight fractionation, ion-exchange adsorption chromatography and hydroxyapatite adsorption chromatography. In the process for purifying the gingival fibroblast chemotactic factor (CCTF) the Mammalian is preferably a bovine.
Inventor(s):	Munekata; Masanobu (Sapporo, JP), Nishimura; Kazuaki (Sakai, JP)
Assignee:	Kanebo, Ltd. (Tokyo, JP)
Application Number:	09/486,020
Patent Claims:	1. A process for purifying a precementum- and/or cementum-derived gingival fibroblast chemotactic factor (CCTF) of a Mammalian tooth, which comprises eluting a protein mixture from precementum and/or cementum of a Mammalian tooth and purifying the precementum- and/or cementum-derived chemotactic factor (CCTF) from the eluted protein mixture by molecular weight fractionation, ion-exchange adsorption chromatography and hydroxyapatite adsorption chromatography. 2. The process for purifying the gingival fibroblast chemotactic factor (CCTF) of claim 4 further comprising the steps of: (a) collecting precementum and/or cementum from an extracted Mammalian tooth and immersing them in saline or collagenase-containing saline with stirring, thereby to elute the protein mixture; (b) removing an insoluble matter from the eluted protein mixture of step (a) by centrifugal separation and filtration, and subjecting the resulting filtrate to gel filtration or ultrafiltration to obtain a fraction having a molecular weight fraction of 270,000 or more; (c) adsorbing the fraction obtained in step (b) on a DEAE ion-exchange resin, collecting a fraction eluted with a 0.2-0.3 mM sodium chloride-containing tris ethanolamine hydrochloride buffer, and concentrating the fraction by desalination; and (d) adsorbing the concentrated fraction obtained in step (c) on hydroxyapatite equilibrated with a phosphate buffer, and collecting a fraction eluted with 80-110 mM phosphoric acid. 3. The process for purifying the gingival fibroblast chemotactic factor (CCTF) according to claim 1 or 2, wherein the Mammalian is a bovine.

Details for Patent 6,429,193

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2017-08-19