Claims for Patent: 6,331,665

Summary for Patent: 6,331,665

Title:	Insect resistant fertile transgenic corn plants
Abstract:	Fertile transgenic Zea mays (corn) plants which stably express recombinant DNA which is heritable are provided wherein said DNA preferably comprises a recombinant gene which encodes a seed storage protein, so that the amino acid profile of the corn is improved.
Inventor(s):	Lundquist; Ronald C. (Minnetonka, MN), Walters; David A. (Bloomington, MN)
Assignee:	DeKalb Genetics Corp. (DeKalb, IL)
Application Number:	08/619,077
Patent Claims:	1. A fertile transgenic Zea mays plant comprising a preselected DNA sequence encoding a Bacillus thuringiensis endotoxin, wherein the preselected DNA sequence is adjusted to be more efficently expressed in maize than the native B. thuringiensis DNA sequence encoding said endotoxin, and wherein said preselected DNA is heritable. 2. The transgenic Zea mays plant of claim 1 wherein the preselected DNA sequence comprises an increased G+C content of the degenerate third base of the codons. 3. The transgenic Zea mays plant of claim 1 or wherein the preselected DNA sequence comprises a sequence encoding the HD73 endotoxin of Bacillus thuringiensis. 4. A seed produced by the transgenic Zea mays plant claim 1, 3, which comprises said preselected DNA sequence. 5. The transgenic Zea mays plant of claim 1 wherein the preselected DNA sequence encodes a truncated Bacillus thuringiensis endotoxin. 6. The transgenic Zea mays plant of claim 1 wherein the preselected DNA sequence comprises a promoter. 7. A fertile inbred transgenic Zea mays plant comprising a preselected DNA sequence encoding a Bacillus thuringiensis endotoxin, wherein the preselected DNA sequence is adjusted to be more efficiently expressed in maize than the native B. thuringiensis DNA sequence encoding said endotoxin and wherein the preselected DNA sequence is heritable. 8. A fertile hybrid transgenic Zea mays plant comprising a preselected DNA sequence encoding a Bacillus thuringiensis endotoxin, wherein the preselected DNA sequence is adjusted to be more efficiently expressed in maize than the native B. thuringiensis DNA sequence encoding said endotoxin, and wherein the preselected DNA sequence is heritable. 9. A fertile transgenic Zea mays plant comprising a preselected heritable DNA sequence encoding a Bacillus thuringiensis endotoxin, wherein the preselected DNA sequence is adjusted to be more efficiently expressed in maize than the native B. thuringiensis DNA sequence encoding said endotoxin, and wherein the preselected DNA sequence further comprises a selectable marker gene or a reporter gene. 10. The transgenic Zea mays plant of claim 9 wherein the preselected DNA sequence comprises a sequence encoding the HD73 endotoxin of Bacillus thuringiensis. 11. The transgenic Zea mays plant of claim 9 wherein the preselected DNA sequence comprises a sequence encoding the HD1 endotoxin of Bacillus thuringiensis. 12. A seed produced by the transgenic Zea mays plant of claim 9, 10 or 11 which comprises said preselected DNA sequence. 13. The transgenic Zea mays plant of claim 9 wherein the DNA sequence encodes a truncated Bacillus thuringiensis endotoxin. 14. The transgenic Zea mays plant of claim 9 wherein the preselected DNA sequence further comprises a promoter operably linked to said DNA sequence encoding said endotoxin and a promoter operably linked to said selectable marker gene. 15. A fertile inbred transgenic Zea mays plant comprising a preselected heritable DNA sequence encoding a Bacillus thuringiensis endotoxin, wherein the preselected DNA sequence is adjusted to be more efficiently expressed in maize than the native B. thuringiensis DNA sequence encoding said endotoxin, and wherein the preselected DNA sequence further comprises a selectable marker gene or a reporter gene. 16. A fertile hybrid transgenic Zea mays plant comprising a preselected heritable DNA sequence encoding a Bacillus thuringiensis endotoxin, wherein the preselected DNA sequence is adjusted to be more efficiently expressed in maize than the native B. thuringiensis DNA sequence encoding said endotoxin, and wherein the preselected DNA sequence further comprises a selectable marker gene or a reporter gene. 17. The transgenic Zea mays plant of claim 9 wherein the selectable marker gene confers resistance or tolerance to a compound selected from the group consisting of hygromycin, sethoxydim, haloxyfop, glyphosate, methotrexate, imidazoline, sulfonylurea, triazolopyrimidine, s-triazine, bromoxynil, phosphinothricin, kanamycin, G418, 2,2-dichloropropionic acid and neomycin. 18. The transgenic plant of claim 17 wherein the compound is phosphinothricin. 19. The transgenic plant of claim 17 wherein the compound is glyphosate. 20. The transgenic plant of claim 17 wherein the compound is kanamycin. 21. The transgenic plant of claim 17 wherein the compound is hygromycin. 22. The transgenic plant of claim 9 wherein the DNA encoding the Bacillus thuringiensis endotoxin is fused in frame with said selectable marker or reporter gene. 23. The inbred transgenic plant of claim 15 wherein the DNA encodes a truncated Bacillus thuringiensis endotoxin. 24. The hybrid transgenic plant of claim 18 wherein the DNA encodes a truncated Bacillus thuringiensis endotoxin. 25. The transgenic plant of claim 5, 23 or 24 wherein the truncated Bacillus thuringiensis endotoxin comprises about the N-terminal 50% of the endotoxin. 26. The transgenic plant of claim 1 or 9 wherein the preselected DNA further encodes a protease inhibitor. 27. The transgenic plant of claim 6 or 14 wherein the preselected DNA sequence further comprises the maize AdhIS first intron or the maize Shrunken-2 first intron positioned between the promoter and the DNA encoding said endotoxin. 28. The transgenic plant of claim 6 or 14 wherein the preselected DNA sequence further comprises a manopine synthase promoter, a nopaline synthase promoter or an octopine synthase promoter. 29. The transgenic plant of claim 6 or 14 wherein the promoter is the CaMV 35S or 19S promoter. 30. A population of plants obtained by breeding the transgenic plants of claim 1 or 14 wherein the preselected DNA sequence is transmitted by Mendelian inheritance through both male and female parent plants. 31. An inbred insect-resistant transgenic Zea mays plant prepared by a process comprising: (a) crossing a fertile transgenic Zea mays plant comprising a preselected DNA sequence encoding a Bacillus thuringiensis endotoxin, wherein the preselected DNA sequence is adjusted to be more efficiently expressed in maize than the a B. thuringiensis DNA sequence encoding said endotoxin, and wherein said DNA sequence is heritable, with a member of a second inbred Zea mays line; (b) recovering insect-resistant transgenic progeny plants from said cross; (c) back-crossing one of the transgenic progeny plant with a member of said second inbred line; (d) recovering insect-resistant transgenic progeny plants from said cross; and (e) repeating steps (b) and (c) to obtain said inbred plant. 32. The inbred transgenic Zea mays plant of claim 31 wherein said preselected DNA sequence encodes a truncated Bacillus thuringiensis endotoxin. 33. A transgenic insect-resistant hybrid plant prepared by crossing the inbred plant of claim 31 or 32 with an inbred Zea mays line, and recovering said hybrid plant.

Details for Patent 6,331,665

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2010-01-22
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2010-01-22
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2010-01-22