Claims for Patent: 6,090,585

Summary for Patent: 6,090,585

Title:	Human collagenase inhibitor sequence, recombinant vector system for using same and recombinant-DNA method for the manufacture of same
Abstract:	A portable DNA sequence is disclosed which is capable of directing intracellular production of metalloproteinanse inhibitors. Vectors containing this portable DNA sequence are also set forth, including the vector pUC9-F5/237P10 (ATCC Accession No. 53003). A recombinant-DNA method for the microbial production of a metalloproteinase inhibitor, which method incorporates at least one of the portable DNA sequences and the vectors disclosed herein is described.
Inventor(s):	Carmichael; David F. (Louisville, CO), Anderson; David C. (Louisville, CO), Stricklin; George P. (Germantown, TN), Welgus; Howard G. (St. Louis, MO)
Assignee:	Amgen Inc. (Thousand Oaks, CA)
Application Number:	08/474,553
Patent Claims:	1. A purified DNA sequence encoding a human collagenase inhibitor, said sequence comprising: 2. A purified DNA sequence encoding a human collagenase inhibitor, said sequence comprising: 10 20 30 40 50 60 GGCCATCGCC GCAGATCCAG CGCCCAGAGA GACACCAGAG AACCCACCAT GGCCCCCTTT 70 80 90 100 110 120 GACCCCTGGC TTCTGCATCC TGTTGTTGCT GTGGCTGATA GCCCCAGCAG GGCCTGCACC 130 140 150 160 170 180 TGTGTCCCAC CCCACCCACA GACGGCCTTC TGCAATTCCG ACCTCGTCAT CAGGGCCAAG 190 200 210 220 230 240 TTCGTGGGGA CACCAGAAGT CAACCAGACC ACCTTATACC AGCGTTATGA GATCAAGATG 250 260 270 280 290 300 ACCAAGATGT ATAAAGGGTT CCAAGCCTTA GGGGATGCCG CTGACATCCG GTTCGTCTAC 310 320 330 340 350 360 ACCCCCGCCA TGGAGAGTGT CTGCGGATAC TTCCACAGGT CCCACAACCG CAGCGAGGAG 370 380 390 400 410 420 TTTCTCATTG CTGGAAAACT GCAGGATGGA CTCTTGCACA TCACTACCTG CAGTTTCGTG 430 440 450 460 470 480 GCTCCCTGGA ACAGCCTGAG CTTAGCTCAG CGCCGGGGCT TCACCAAGAC CTACACTGTT 490 500 510 520 530 540 GGCTGTGAGG AATGCACAGT GTTTCCCTGT TTATCCATCC CCTGCAAACT GCAGAGTGGC 550 560 570 580 590 600 ACTCATTGCT TGTGGACGGA CCAGCTCCTC CAAGGCTCTG AAAAGGGCTT CCAGTCCCGT 610 620 630 640 650 660 CACCTTGCCT GCCTGCCTCG GGAGCCAGGG CTGTGCACCT GGCAGTCCCT GCGGTCCCAG 670 680 690 700 710 720 ATAGCCTGAA TCCTGCCCGG AGTGGAAGCT GAAGCCTGCA CAGTGTCCAC CCTGTTCCCA 730 740 750 760 770 780 CTCCCATCTT TCTTCCGGAC AATGAAATAA AGAGTTACCA CCCAGCAAAA AAAAAAAGGA. 3. A recombinant-DNA cloning vector comprising a nucleotide sequence encoding a human collagenase inhibitor, said nucleotide sequence comprising: 10 20 30 40 50 60 GTTGTTGCTG TGGCTGATAG CCCCAGCAGG GCCTGCACCT GTGTCCCACC CCACCCACAG 70 80 90 100 110 120 ACGGCCTTCT GCAATTCCGA CCTCGTCATC AGGGCCAAGT TCGTGGGGAC ACCAGAAGTC 130 140 150 160 170 180 AACCAGACCA CCTTATACCA GCGTTATGAG ATCAAGATGA CCAAGATGTA TAAAGGGTTC 190 200 210 220 230 240 CAAGCCTTAG GGGATGCCGC TGACATCCGG TTCGTCTACA CCCCCGCCAT GGAGAGTGTC 250 260 270 280 290 300 TGCGGATACT TCCACAGGTC CCACAACCGC AGCGAGGAGT TTCTCATTGC TGGAAAACTG 310 320 330 340 350 360 CAGGATGGAC TCTTGCACAT CACTACCTGC AGTTTCGTGG CTCCCTGGAA CAGCCTGAGC 370 380 390 400 410 420 TTAGCTCAGC GCCGGGGCTT CACCAAGACC TACACTGTTG GCTGTGAGGA ATGCACAGTG 430 440 450 460 470 480 TTTCCCTGTT TATCCATCCC CTGCAAACTG CAGAGTGGCA CTCATTGCTT GTGGACGGAC 490 500 510 520 530 540 CAGCTCCTCC AAGGCTCTGA AAAGGGCTTC CAGTCCCGTC ACCTTGCCTG CCTGCCTCGG 550 560 570 580 590 600 GAGCCAGGGC TGTGCACCTG GCAGTCCCTG CGGTCCCAGA TAGCCTGAAT CCTGCCCGGA 610 620 630 640 650 660 GTGGAAGCTG AAGCCTGCAC AGTGTCCACC CTGTTCCCAC TCCCATCTTT CTTCCGGACA 670 680 690 700 ATGAAATAAA GAGTTACCAC CCAGCAAAAA AAAAAAGGAA TTC. 4. A recombinant-DNA cloning vector comprising a nucleotide sequence encoding a human collagenase inhibitor, said nucleotide sequence comprising: 10 20 30 40 50 60 GGCCATCGCC GCAGATCCAG CGCCCAGAGA GACACCAGAG AACCCACCAT GGCCCCCTTT 70 80 90 100 110 120 GACCCCTGGC TTCTGCATCC TGTTGTTGCT GTGGCTGATA GCCCCAGCAG GGCCTGCACC 130 140 150 160 170 180 TGTGTCCCAC CCCACCCACA GACGGCCTTC TGCAATTCCG ACCTCGTCAT CAGGGCCAAG 190 200 210 220 230 240 TTCGTGGGGA CACCAGAAGT CAACCAGACC ACCTTATACC AGCGTTATGA GATCAAGATG 250 260 270 280 290 300 ACCAAGATGT ATAAAGGGTT CCAAGCCTTA GGGGATGCCG CTGACATCCG GTTCGTCTAC 310 320 330 340 350 360 ACCCCCGCCA TGGAGAGTGT CTGCGGATAC TTCCACAGGT CCCACAACCG CAGCGAGGAG 370 380 390 400 410 420 TTTCTCATTG CTGGAAAACT GCAGGATGGA CTCTTGCACA TCACTACCTG CAGTTTCGTG 430 440 450 460 470 480 GCTCCCTGGA ACAGCCTGAG CTTAGCTCAG CGCCGGGGCT TCACCAAGAC CTACACTGTT 490 500 510 520 530 540 GGCTGTGAGG AATGCACAGT GTTTCCCTGT TTATCCATCC CCTGCAAACT GCAGAGTGGC 550 560 570 580 590 600 ACTCATTGCT TGTGGACGGA CCAGCTCCTC CAAGGCTCTG AAAAGGGCTT CCAGTCCCGT 610 620 630 640 650 660 CACCTTGCCT GCCTGCCTCG GGAGCCAGGG CTGTGCACCT GGCAGTCCCT GCGGTCCCAG 670 680 690 700 710 720 ATAGCCTGAA TCCTGCCCGG AGTGGAAGCT GAAGCCTGCA CAGTGTCCAC CCTGTTCCCA 730 740 750 760 770 780 CTCCCATCTT TCTTCCGGAC AATGAAATAA AGAGTTACCA CCCAGCAAAA AAAAAAAGGA. 5. The vector pUC9-F5/237P10. 6. A recombinant-DNA method for microbial production of a human collagenase inhibitor comprising: (a) culturing a host microorganism under conditions wherein said inhibitor is expressed, said microorganism containing the vector of claim 3, said vector comprising a nucleotide sequence encoding a human collagenase inhibitor; and (b) harvesting said inhibitor. 7. A recombinant-DNA method for microbial production of a human collagenase inhibitor comprising: (a) culturing a host microorganism under conditions wherein said inhibitor is expressed, said microorganism containing the vector of claim 4, said vector comprising a nucleotide sequence encoding a human collagenase inhibitor; and (b) harvesting said inhibitor. 8. The microorganism C600/pUC9-F5/237P10 having A.T.C.C. accession number 53003.

Details for Patent 6,090,585

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Smith & Nephew, Inc.	SANTYL	collagenase	Ointment	101995	06/04/1965		2017-07-18