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Last Updated: May 7, 2024

Claims for Patent: 5,723,332

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Summary for Patent: 5,723,332

Title:	Translational enhancer DNA
Abstract:	The gene which expresses the light (.beta.-)chain of kinesin contains at its 5\'-end a region of DNA of high secondary structure which provides a strong enhancer of translation. In the human kinesin gene it forms a double hairpin loop. The secondary structure, optionally with flanking sequence, is claimed as isolated DNA. Constructs in which it serves is an enhancer for expression of foreign DNA are also claimed.
Inventor(s):	Chernajovsky; Yuti Luis Alberto (London, GB2)
Assignee:	British Technology Group Limited (London, GB2)
Application Number:	08/633,779
Patent Claims:	1. Isolated DNA from the portion of the .beta.-chain of human kinesin genomic DNA lying between the 5'-end of the gene and the 3'-end of the first intron thereof, comprising nucleotides 430 to 474 of SEQ ID NO:1 or a variant thereof, wherein one to four nucleotides of said 430-474 nucleotides are varied or deleted. 2. DNA according to claim 1, which comprises nucleotides 430 to 551 of SEQ ID NO:1 or a said variant thereof. 3. DNA according to claim 2 which comprises, in addition to said nucleotides 430 to 551 or a said variant thereof, from 1 to 100 nucleotides of 5'- or 3'- flanking sequence of said .beta.-chain of human kinesin genomic DNA. 4. DNA according to claim 1 which comprises, in addition to said nucleotides 430 to 474 or a said variant thereof, from 1 to 100 nucleotides of 5'- or 3'-flanking sequence of said .beta.-chain of human kinesin genomic DNA. 5. Isolated DNA according to claim 1 which further comprises an open-reading frame from nucleotides 391-426 of SEQ ID NO:1 coding for protein of 12 amino acids. 6. A construct for expression of non-.beta.-kinesin DNA encoding a protein, comprising in the order 5' to 3' (1) a eukaryotic promoter, (2) DNA according to claim 1 for enhancement of translation and (3) DNA coding for a protein other than .beta.-kinesin. 7. A construct for expression of two protein coding DNAs which comprises in the order 5' to 3' (1) a eukaryotic promoter, (2) downstream of the promoter, DNA coding for a first protein other than .beta.-kinesin and which lacks a transcription termination signal, (3) DNA according to claim 6 which serves both as an internal ribosome entry site (IRES) and a translational enhancer and (4) DNA coding for a second protein other than .beta.-kinesin and which is distanced downstream from the IRES-translational enhancer DNA so as to permit translation of mRNA from the IRES. 8. A construct according to claim 6 wherein the promoter is one which is stronger than that of the native .beta.-kinesin.

Details for Patent 5,723,332

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Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986	⤷ Try a Trial	2013-11-26
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132		⤷ Try a Trial	2013-11-26
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132		⤷ Try a Trial	2013-11-26
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

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