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Last Updated: March 28, 2024

Claims for Patent: 5,712,155


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Summary for Patent: 5,712,155
Title: DNA encoding tumor necrosis factor-.alpha. and -.beta. receptors
Abstract:Tumor necrosis factor receptor DNAs and expression vectors encoding TNF receptors, and processes for producing TNF receptors as products of recombinant cell culture, are disclosed.
Inventor(s): Smith; Craig A. (Seattle, WA), Goodwin; Raymond G. (Seattle, WA), Beckmann; M. Patricia (Poulsbo, WA)
Assignee: Immunex Corporation (Seattle, WA)
Application Number:08/346,555
Patent Claims:1. An isolated DNA sequence selected from the group consisting of:

(a) a DNA sequence that encodes a polypeptide having the amino acid sequence selected from the group consisting of amino acids 1 to X of FIG. 2A and amino acids 1 to 233 of FIG. 3A, wherein X is an amino acid from 163 to 235; and

(b) a DNA sequence capable of hybridization to the complement of the DNA sequence of (a) under moderately stringent conditions (50.degree. C., 2.times. SSC) and which encodes a polypeptide that is capable of binding to TNF and which is at least 88% identical to a polypeptide encoded by the DNA of (a).

2. An isolated DNA sequence selected from the group consisting off

(a) a DNA sequence that encodes a polypeptide having the amino acid sequence selected from the group consisting of amino acids 1 to X of FIG. 2A and amino acids 1 to 233 of FIG. 3A, wherein X is an amino acid from 163 to 235; and

(b) a DNA sequence capable of hybridization to the complement of the DNA sequence of (a) under moderately stringent conditions (50.degree. C., 2.times. SSC) and which encodes TNF-R protein that is capable of binding greater than 0.1 nmoles TNF per nmole TNF-R and which is at least 88% identical to a polypeptide encoded by the DNA of (a).

3. An isolated DNA sequence selected from the group consisting of:

(a) a DNA sequence that encodes a polypeptide having the amino acid sequence selected from the group consisting of amino acids 1 to X of FIG. 2A and amino acids 1 to 233 of FIG. 3A, wherein X is an amino acid from 163 to 235; and

(b) a DNA sequence capable of hybridization to the complement of the DNA sequence of (a) under moderately stringent conditions (50.degree. C., 2.times. SSC) and which encodes TNF-R protein that is capable of binding greater than 0.5 nmoles TNF per nmole TNF-R and which is at least 88% identical to a polypeptide encoded by the DNA of (a).

4. A recombinant expression vector comprising the DNA sequence according to claim 1.

5. A recombinant expression vector comprising the DNA sequence according to claim 2.

6. A recombinant expression vector comprising the DNA sequence according to claim 3.

7. A host cell transformed or transfected with the vector according to claim 4.

8. A host cell transformed or transfected with the vector according to claim 5.

9. A host cell transformed or transfected with the vector according to claim 6.

10. An isolated DNA sequence selected from the group consisting of:

(a) a DNA sequence that encodes a polypeptide having the amino acid sequence selected from the group consisting of amino acids 1 to X of FIG. 2A and amino adds 1 to 233 of FIG. 3A, wherein X is an amino acid from 163 to 235; and

(b) a DNA sequence that encodes a polypeptide identical to the polypeptide encoded by the DNA of (a) except for modification(s) to the amino acid sequence selected from the group consisting of: (i) inactivated N-linked glycosylation sites; (ii) altered KEX2 protease cleavage sites; (iii) conservative amino acid substitutions; (iv) substitution or deletion of cysteine residues; and (iv) combinations of modifications (i)-(iv); wherein such polypeptide is capable of binding TNF.

11. An isolated DNA sequence selected from the group consisting of:

(a) a DNA sequence that encodes a polypeptide having the amino acid sequence selected from the group consisting of amino acids 1 to X of FIG. 2A and amino acids 1 to 233 of FIG. 3A, wherein X is an amino acid from 163 to 235; and

(b) a DNA sequence that encodes a polypeptide identical to the polypeptide encoded by the DNA of (a) except for modification(s) to the amino acid sequence selected from the group consisting of: (i) inactivated N-linked glycosylation sites; (ii) altered KEX2 protease cleavage sites; (ii) conservative amino acid substitutions; (iv) substitution or deletion of cysteine residues; and (v) combinations of modifications (i)-(iv); which encoded polypeptide is capable of binding greater than 0.1 moles TNF per nmole of such polypeptide.

12. An isolated DNA sequence selected from the group consisting of:

(a) a DNA sequence that encodes a polypeptide having the amino acid sequence selected from the group consisting of amino acids 1 to X of FIG. 2A and amino acids 1 to 233 of FIG. 3A, wherein X is an amino acid from 163 to 235; and

(b) a DNA sequence that encodes a polypeptide identical to the polypeptide encoded by the DNA of (a) except for modification(s) to the amino acid sequence selected from the group consisting of: (i) inactivated N-linked glycosylation sites; (ii) altered KEX2 protease cleavage sites; (ii) conservative amino acid substitutions; (iv) substitution or deletion of cysteine residues; and (v) combinations of modifications (i)-(iv); which encoded polypeptide is capable of binding greater than 0.5 moles TNF per nmole of such polypeptide.

13. A recombinant expression vector comprising the DNA according to any of claims 10, 11 or 12.

14. A host cell transformed or transfected with the vector according to claim 13.

15. A DNA sequence that encodes a polypeptide having the amino acid sequence selected from the group consisting of (a) amino acids 1-235 of FIG. 2A; and (b) a DNA sequence capable of hybridization to the DNA sequence of (a) under moderately stringent conditions (50.degree. C., 2.times. SSC) and which encodes a polypeptide that is capable of binding to TNF and which is at least 88% identical to a polypeptide encoded by the DNA of (a).

16. A recombinant expression vector comprising the DNA sequence according to claim 15.

17. A host cell transformed or transfected with the vector according to claim 16.

Details for Patent 5,712,155

Applicant Tradename Biologic Ingredient Dosage Form BLA Approval Date Patent No. Expiredate
Immunex Corporation ENBREL etanercept For Injection 103795 11/02/1998 ⤷  Try a Trial 2009-09-05
Immunex Corporation ENBREL etanercept For Injection 103795 05/27/1999 ⤷  Try a Trial 2009-09-05
Immunex Corporation ENBREL etanercept Injection 103795 09/27/2004 ⤷  Try a Trial 2009-09-05
Immunex Corporation ENBREL etanercept Injection 103795 02/01/2007 ⤷  Try a Trial 2009-09-05
Immunex Corporation ENBREL MINI etanercept Injection 103795 09/14/2017 ⤷  Try a Trial 2009-09-05
Immunex Corporation ENBREL etanercept Injection 103795 ⤷  Try a Trial 2009-09-05
>Applicant >Tradename >Biologic Ingredient >Dosage Form >BLA >Approval Date >Patent No. >Expiredate

International Patent Family for US Patent 5,712,155

Country Patent Number Estimated Expiration
South Africa 907072 ⤷  Try a Trial
World Intellectual Property Organization (WIPO) 9406476 ⤷  Try a Trial
World Intellectual Property Organization (WIPO) 9319777 ⤷  Try a Trial
World Intellectual Property Organization (WIPO) 9103553 ⤷  Try a Trial
United States of America RE36755 ⤷  Try a Trial
United States of America 7459528 ⤷  Try a Trial
>Country >Patent Number >Estimated Expiration

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