Claims for Patent: 5,695,968

Summary for Patent: 5,695,968

Title:	Process for production of D-.alpha.-amino acids
Abstract:	The present invention is directed to a gene which is related to a D-N-carbamoyl-.alpha.-amino acid amidohydrolase which is an enzyme capable of converting D-N-carbamoyl-.alpha.-amino acids into D-.alpha.-amino acids; a recombinant plasmid in which a DNA fragment containing the gene is incorporated into a vector; a microorganism belonging to the genus Escherichia, Pseudomonas, Flavobacterium, Bacillus, Serratia, Corynebacterium, or Brevibacterium, which is transformed by incorporating the recombinant plasmid thereinto; a process for the production of D-N-carbamoyl-.alpha.-amino acid amidohydrolases, comprising the steps of cultivating the transformed microorganism and collecting the desired product therefrom; a D-N-carbamoyl-.alpha.-amino acid amidohydrolase obtained by the method; and a process for the production of D-.alpha.-amino acids with the aid of an action of the enzyme. The D-N-carbamoyl-.alpha.-amino acid amidohydrolase can be fixed on a support for immobilization and used as an immobilized enzyme.
Inventor(s):	Nanba; Hirokazu (Takasago, JP), Yamada; Yukio (Kakogawa, JP), Takano; Masayuki (Akashi, JP), Ikenaka; Yasuhiro (Akashi, JP), Takahashi; Satomi (Kobe, JP), Yajima; Kazuyoshi (Kobe, JP)
Assignee:	Kanegafuchi Kagaku Kogyo Kabushiki Kaisha (Osaka, JP)
Application Number:	08/479,638
Patent Claims:	1. A process for the production of D-.alpha.-amino acids characterized in that D-N-carbamoyl-.alpha.-amino acids represented by the formula: R--CH(NHCONH.sub.2)--COOH wherein R is phenyl; phenyl substituted with hydroxy; alkyl having 1 to 4 carbon atoms; alkyl having 1 to 4 carbon atoms substituted with hydroxy, alkylthio, carboxyl, amino, phenyl or phenyl substituted with hydroxy or amido; aralkyl having 7 to 8 carbon atoms; or thienyl; provided that when R is phenyl substituted with hydroxyl, optionally one or more hydroxyl groups are attached at any of the o-, m-, and p-positions; are converted into the corresponding D-.alpha.-amino acids in an aqueous medium, using an enzyme which is produced by a transformant obtained by transforming a host bacterial cell with a recombinant DNA comprising a vector and a DNA fragment having a gene encoding said enzyme, said enzyme being capable of converting D-N-carbamoyl-.alpha.-amino acids by removal of their carbamoyl groups into the corresponding D-.alpha.-amino acids, after which the D-.alpha.-amino acids are collected, wherein the recombinate DNA is a plasmid selected from the group consisting of pAHD 101, pAD 108, pPHD 301, and pPD 304. 2. A process for the production of D-.alpha.-amino acids characterized in that D-N-carbamoyl-.alpha.-amino acids represented by the formula: R--CH(NHCONH.sub.2)--COOH wherein R is phenyl; phenyl substituted with hydroxy; alkyl having 1 to 4 carbon atoms; alkyl having 1 to 4 carbon atoms substituted with hydroxy, alkylthio, carboxyl, amino, phenyl or phenyl substituted with hydroxy or amido; aralkyl having 7 to 8 carbon atoms; or thienyl; provided that when R is phenyl substituted with hydroxyl, one or more hydroxyl groups are attached at any of the o-, m-, and p-positions; are converted into the corresponding D-.alpha.-amino acids in an aqueous medium, using an enzyme which is produced by a transformant obtained by transforming a host bacterial cell with a recombinant DNA comprising a vector and a DNA fragment having a gene encoding a D-N-carbamoyl-.alpha.-amino acid amidohydrolase capable of converting D-N-carbamoyl-.alpha.-amino acids by removal of their carbamoyl groups into the corresponding D-.alpha.-amino acids, after which the D-.alpha.-amino acids are collected, wherein said transformant is selected from the group consisting of Escherichia coli JM 109 pAHD 101, Escherichia coli JM 109 pAD 108 (FERM BP-3184), Escherichia coli JM 109 pPHD 301 and Escherichia coli JM 109 pPD 304 (FERM BP-3183). 3. A process for the production of a D-.alpha.-amino acids characterized in that D-N-carbamoyl-.alpha.-amino acids represented by the formula: wherein R is phenyl; phenyl substituted with hydroxy; alkyl having 1 to 4 carbon atoms; alkyl having 1 to 4 carbon atoms substituted with hydroxy, alkylthio, carboxyl, amino, phenyl or phenyl substituted with hydroxy or amido; aralkyl having 7 to 8 carbon atoms; or thienyl; provided that when R is phenyl substituted with hydroxyl, optionally one or more hydroxyl groups are attached at any of the o-, m-, and p-positions; are converted into the corresponding D-.alpha.-amino acids in an aqueous medium, using an enzyme which is produced by a bacterium selected from the group consisting of Pseudomonas sp. KNK 0030A (FERM BP-3181), and Pseudomonas sp. KNK 505 (FERM BP-3182), said enzyme being capable of converting D-N-carbamoyl-.alpha.-amino acids by removal of their carbamoyl groups into the corresponding D-.alpha.-amino acids, after which the D-.alpha.-amino acids are collected.

Details for Patent 5,695,968

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Baxalta Us Inc.	BIOCLATE (ARMOUR), RECOMBINATE	antihemophilic factor (recombinant)	For Injection	103375	12/10/1992		2014-12-09
Baxalta Us Inc.	BIOCLATE (ARMOUR), RECOMBINATE	antihemophilic factor (recombinant)	For Injection	103375	03/10/2010		2014-12-09