Claims for Patent: 5,691,295

Summary for Patent: 5,691,295

Title:	Detergent compositions
Abstract:	A laundry detergent for washing fabrics composed of proteinogenic fibers is comprised of at least one surfactant and a proteolytically active amount of a protease having a keratinase/caseinase activity ratio of less than about 0.80.
Inventor(s):	Maurer; Karl-Heinz (Erkrath, DE), Pochandke; Winfried (Monheim, DE), Kottwitz; Beatrix (Duesseldorf, DE), Poethkow; Jorg (Duesseldorf, DE), Weiss; Albrecht (Langenfold, DE), Schmidt; Irmgard (Solingen, DE), Upadek; Horst (Ratingen, DE)
Assignee:	Cognis Gesellschaft fuer Biotechnologie mbH (Duesseldorf, DE)
Application Number:	08/373,818
Patent Claims:	1. A detergent composition for washing fabrics composed of proteinogenic fibers comprising at least one surfactant and a proteolytically active amount of a Bacillus lentus protease selected from the group consisting of F49 variant having S3T+V4I+A188P+V193M+V199I+L211D mutations, F46 variant having S3T+V4I+S153E+A188P+V193M+V199I or combinations thereof having a keratinase/caseinase activity ratio of less than about 0.80. 2. The detergent of claim 1 wherein said protease has a KC ratio of less than about 0.70. 3. The detergent of claim 1 wherein said ratio is from about 0.2 to about 0.65. 4. The detergent of claim 1 wherein said protease has a proteolytic activity of from about 100 PU/g to about 7500 PU/g. 5. The detergent of claim 1 wherein said protease is mutant BacillUs lantus DSM 5483 variant F49 having the following mutations: S3T +V4I +A188P+V193M+V199I+L211D. 6. A detergent composition for washing fabrics composed of proteinogenic fibers comprising a proteolytically active amount of a Bacillus lentus protease selected from the group consisting of F49 variant having S3T+V4I+A188P+V193M+V199I+L211D mutations, F46 variant having 3ST+V4I+S154E+A188P+V193M+V199I or combination thereof having a keratinase/caseinase activity ratio of less than about 0.80 and a nonionic surfactant selected from the group consisting of a fatty alkyl polyglycoside, a fatty alkyl polyalkoxylate, a fatty acid polyhydroxyamide, an ethoxylated fatty alkylamine, a propoxylated fatty alkylamine, an ethoxylated vicinal diol, a propoxylated vicinal diol, an ethoxylated fatty acid alkyl ester, a propoxylated fatty acid alkyl ester, an ethoxylated fatty acid amide, a propoxylated fatty acid amide, and mixtures thereof. 7. The detergent of claim 6 wherein said nonionic surfactant is present in an amount of from about 2% to about 25% by weight. 8. The detergent of claim 6 further comprising an anionic surfactant selected from the group consisting of a fatty alkyl sulfate, a fatty alkyl ether sulfate, a sulfofatty acid ester, a sulfofatty acid disalt, and combinations thereof. 9. A detergent composition for washing fabrics composed of proteinogenic fibers comprising a proteolytically active amount of a Bacillus lentus protease selected from the group consisting of F49 variant having S3T+V4I+A188P+V193M+V199I+L211D, F46 variant having 3ST+V4I+S154E+A188P+V193M+V199I mutations and combinations thereof having a keratinase/caseinase activity ratio of less than about 0.80 and an anionic surfactant selected from the group consisting of a fatty alkyl sulfate, a fatty alkyl ether sulfate, a sulfofatty acid ester, a sulfofatty acid disalt, and combinations thereof. 10. The detergent of claim 6 wherein said anionic surfactant is present in an amount of from about 2% to about 25% by weight. 11. The detergent of claim 9 wherein said anionic surfactant is selected from the group consisting of an alkyl sulfate, an alkenyl sulfate, an alkyl ether sulfate, an alkenyl ether sulfate in wherein the alkyl or alkenyl group contains from about 8 to about 22 carbon atoms. 12. The detergent of claim 11 wherein said alkyl or alkenyl group contains from about 12 to about 18 carbon atoms. 13. The detergent of claim 1 further comprising a builder selcted from the group consisting of an alkali metal alumosilicate, a crystalline alkali metal silicate with a modulus above 1, a monomeric polycarboxylate, a polymeric polycarboxylate and mixtures thereof. 14. The detergent of claim 13 wherein said builder is present in an amount of from about 2.5% to about 60% by weight. 15. A detergent composition for washing fabrics composed of proteinogenic fibers comprising from about 20% to about 55% by weight of an inorganic builder; up to about 15% by weight of a water-soluble organic builder; from about 2.5% to about 20% by weight of an anionic surfactant; from about 1% to about 20% by weight of a nonionic surfactant; up to about 25% by weight of a bleaching agent; up to about 8% by weight of a bleach activator; up to about 20% by weight of an inorganic salt; from about 0.4% to about 1.2% by weight of a lipase, cutinase, amylase, cellulase, pullulanase, oxidase or peroxidase and a proteolytically aistive amount of a Bacillus lentus protease selected from the group consisting of F49 variant having S3T+V4I+A188P+V193M+V199I+L211D mutations, F46 variant having S3T+V4I+S154E+A188P+V193M+V199I mutations, or combinations thereof having a keratinase/caseinase activity ratio of less than about 0.80. 16. The detergent of claim 15 wherein the amount of said organic builder is from about 2% to about 12% by weight; the amount of said bleaching agent is from about 1% to about 15% by weight; the amount of said bleach activator is from about 0.5% to about 6% by weight; the amount of said inorganic salt is from about 0.1% to about 15% by weight; the amount of said lipase, cutinase, amylase, cellulase, pullulanase, oxidase or peroxidase is from about 0.4% to about 1.2% by weight. 17. The detergent of claim 16 wherein said inorganic salt is an alkali metal carbonate, an alkali metal hydrogen carbonate, or an alkali metal sulfate. 18. A detergent composition for washing fabrics composed of proteinogenic fibers comprising from about 20% to about 55% by weight of an inorganic builder; up to about 15% by weight of a water-soluble organic builder; up to about 15% by weight of an anionic surfactant; from about 4% to about 24% by weight of a nonionic surfactant; up to about 65% by weight of an inorganic salt; and a proteolytically active amount of a Bacillus lentus protease selected from the group consisting of F49 variant having S3T+V4I+A188P+V193M+V199I+L211D mutation, F46 having S3T+V4I+S154E+A188P+V193M+V199I mutations or combinations thereof having a keratinase/caseinase activity ratio of less than about 0.80. 19. The detergent of claim 18 wherein the amount of said organic builder is from about 2% to about 12% by weight; the amount of said anionic surfactant is from about 1% to about 10% by weight; the amount of said inorganic salt is from about 1% to about 30% by weight. 20. A composition comprising first and second mutant proteases wherein in said first mutant protease at least one of the amino acid residues at positions 99 and 154 are different from those in Bacillus lentus DSM 5483 and wherein in said second mutant protease at least the amino acid residue at position 211 is different from that in Bacillus lentus DSM 5483. 21. The composition of claim 20 wherein said first mutant protease is F46. 22. The composition of claim 20 wherein said second mutant protease is F49. 23. A detergent composition comprising at least one surfactant and at least a first and second mutant protease wherein in said first mutant protease at least one of the amino acid residues at positions 99 and 154 are different from those in Bacillus lentus DSM 5483 and wherein in said second mutant protease at least the amino acid residue at position 211 is different from that in Bacillus lentus DSM 5483. 24. A composition comprising at least one surfactant and a proteolytically active amount of first and second mutant proteases wherein in said first mutant protease at least one of the amino acid residues at positions 99 and 154 are different from those in Bacillus lentus DSM 5483 and wherein in said second mutant protease at least the amino acid residue at position 211 is different from that in Bacillus lentus DSM 5483. 25. The composition of claim 24 wherein said first mutant protease is F46 having the following mutations: S3T+V4I+S154E+A188P+V193M+V199I. 26. The composition of claim 24 wherein said second mutant protease is F49 having the following mutations: S3T+V4I+A188P+V193M+V199I+L211D. 27. A composition comprising at least one surfactant and a proteolytically active amount of first and second mutant Bacillus lentus proteases wherein each of said protease has an amino acid subsitution selected from the group consisting of R99G, R99A, R99S, S154D, S154E, L221 D, L211 E, and a combination thereof.

Details for Patent 5,691,295

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Sanofi-aventis U.s. Llc	LANTUS	insulin glargine	Injection	021081	April 20, 2000	5,691,295	2015-01-17
Sanofi-aventis U.s. Llc	LANTUS	insulin glargine	Injection	021081	April 25, 2007	5,691,295	2015-01-17