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Last Updated: April 26, 2024

Claims for Patent: 5,658,759

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Summary for Patent: 5,658,759

Title:	Recombinant DNA expression vectors
Abstract:	The invention provides expression vectors containing the promoter, enhancer and substantially complete 5\'-untranslated region including the first intron of the major immediate early gene of human cytomegalovirus. Further vectors including the hCMV-MIE DNA linked directly to the coding sequence of a heterologous gene are described. Host cells transfected with the vectors and a process for producing heterologous polypeptides using the vectors and the use of the hCMV-MIE DNA for expression of a heterologous gene are also included within the invention.
Inventor(s):	Bebbington; Christopher Robert (Windsor, GB)
Assignee:	Celltech Limited (Berkshire, GB)
Application Number:	08/633,013
Patent Claims:	1. A host cell transfected with a recombinant expression vector comprising the promoter, enhancer and complete 5' untranslated region including the first intron of the hCMV-MIE operably linked to a heterologous coding sequence. 2. A process for the recombinant production of a heterologous polypeptide comprising culturing a host cell according to claim 1. 3. A host cell according to claim 1, wherein the recombinant expression vector further comprises a restriction site to facilitate insertion of the heterologous coding sequence. 4. A host cell according to claim 1, wherein the promoter, enhancer and complete 5' untranslated region including the first intron of the hCMV-MIE gene are linked directly to the heterologous coding sequence. 5. A host cell according to claim 1, wherein the recombinant expression vector further includes the translational initiation signal of the hCMV-MIE gene. 6. A host cell according to claim 1, wherein the recombinant expression vector is pCMGS, pEE6.hCMV or pHT.1. 7. A process for the recombinant production of a heterologous polypeptide comprising culturing a host cell according to claim 3. 8. A process for the recombinant production of a heterologous polypeptide comprising culturing a host cell according to claim 4. 9. A process for the recombinant production of a heterologous polypeptide comprising culturing a host cell according to claim 5. 10. A process for the recombinant production of a heterologous polypeptide comprising culturing a host cell according to claim 6.

Details for Patent 5,658,759

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Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986	⤷ Try a Trial	2007-07-23
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132		⤷ Try a Trial	2007-07-23
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132		⤷ Try a Trial	2007-07-23
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

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