Claims for Patent: 5,622,851

Summary for Patent: 5,622,851

Title:	Human deoxycytidylate deaminase gene
Abstract:	The present invention is directed to a DNA molecule encoding a gene for human deoxycytidylate deaminase. The DNA molecule comprises 26,764 base pairs. A 1317 base pair 5\' untranslated region is followed by exon 1 of 108 base pairs, which is followed by intron 1 of 402 base pairs, which is followed by exon 2 of 136 base pairs, which is followed by intron 2 of 20,303 base pairs, which is followed by exon 3 of 117 base pairs, which is followed by intron 3 of 1357 base pairs, which is followed by exon 4 of 97 base pairs, which is followed by intron 4 of 1554 base pairs, which is followed by exon 5 of 76 base pairs, which is followed by a 3\' untranslated region of 1297 base pairs. Methods of using the DNA molecule are also provided.
Inventor(s):	Maley; Frank (Delmar, NY), Maley; Gladys R. (Delmar, NY), Weiner; Karen X. B. (Syracuse, NY)
Assignee:	Health Research, Incorporated (Albany, NY)
Application Number:	08/370,975
Patent Claims:	1. An isolated DNA molecule encoding a gene for human deoxycytidylate deaminase comprising a 5' untranslated region, said 5' untranslated region having a nucleotide sequence as shown in SEQ ID NO:2. 2. An isolated DNA molecule selected from the group consisting of intron 1 having a nucleotide sequence as shown in SEQ ID NO:4, intron 2 having a nucleotide sequence as shown in SEQ ID NO:6, intron 3 having a nucleotide sequence as shown in SEQ ID NO:8, and intron 4 having a nucleotide sequence as shown in SEQ ID NO:10. 3. An isolated DNA molecule encoding a gene for human deoxycytidylate deaminase wherein said DNA molecule has a nucleotide sequence comprising SEQ ID NO:1. 4. An expression vector comprising the DNA molecule of claim 3. 5. A cell transfected with the expression vector of claim 4. 6. A cell comprising the DNA molecule of claim 3. 7. A method for producing human deoxycytidylate deaminase which method comprises culturing the cell of claim 6 under conditions suitable for translation of said DNA molecule, thereby expressing said human deoxycytidylate deaminase, and recovering the human deoxycytidylate deaminase expressed thereby. 8. An oligonucleotide having a nucleotide sequence complementary to the nucleotide sequence of the isolated DNA molecule of claim 2.

Details for Patent 5,622,851

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986		2039-02-26
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132			2039-02-26
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132			2039-02-26