You’re using a public version of DrugPatentWatch with 5 free searches available | Register to unlock more free searches. CREATE FREE ACCOUNT

Last Updated: May 10, 2024

Claims for Patent: 5,462,873

✉ Email this page to a colleague

« Back to Dashboard

Summary for Patent: 5,462,873

Title:	DNA for use in a simple method for detecting inhibitors of retrotransposition
Abstract:	The present invention relates, in general, to a DNA segment. In particular, the present invention relates to a DNA segment comprising a selectable marker gene, a DNA segment comprising a selectable marker gene inserted into a retrotransposon, cells containing these DNA segments, and methods of using these DNA segments and cells.
Inventor(s):	Garfinkel; David J. (Frederick, MD), Curcio; M. Joan (Keedysville, MD)
Assignee:	The United States of America as represented by the Department of Health (Washington, DC)
Application Number:	08/102,854
Patent Claims:	1. A vector comprising a DNA segment comprising a selectable marker gene wherein said selectable marker gene contains an intron inserted into a coding sequence of said gene and said intron is present in an antisense orientation relative to transcription of said marker gene. 2. The DNA segment according to claim 1, wherein said selectable marker gene is HIS3 containing said intron. 3. The DNA segment according to claim 2, wherein said selectable marker gene is HIS3AI. 4. A cell that is transformed with a DNA segment according to claim 1. 5. A vector comprising a DNA segment comprising a selectable marker gene inserted into a retrotransposon wherein said selectable marker gene contains an intron inserted into a coding sequence of said gene and said intron is in an antisense orientation relative to transcription of said marker gene and in a sense orientation relative to transcription of said retrotransposon. 6. The DNA segment according to claim 5, wherein said intron is an artificial intron. 7. The DNA segment according to claim 6, wherein said artificial intron is a yeast artificial intron. 8. The DNA segment according to claim 7, wherein said yeast artificial intron is a 104-base-pair artificial yeast intron contained on a PvuII-SnaB1 fragment. 9. The DNA segment according to claim 5, wherein said selectable marker gene is HIS3 containing said intron. 10. The DNA segment according to claim 9, wherein said selectable marker gene is HIS3AI. 11. The DNA segment according to claim 10, wherein said DNA segment is Ty1-H3mHIS3A1. 12. The DNA segment according to claim 11, wherein said DNA segment is pGTy1-H3mHIS3A1. 13. The DNA segment according to claim 5, wherein said retrotransposon is a yeast retrotransposon. 14. The DNA segment according to claim 13, wherein said yeast retrotransposon is Ty1. 15. A cell that is transformed with a DNA segment according to claim 5. 16. The cell according to claim 15, wherein said cell is a eukaryotic cell. 17. The cell according to claim 16, wherein said eukaryotic cell is a yeast cell.

Details for Patent 5,462,873

Subscribe to access the full database, or Try a Trial
Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132	06/04/1986	⤷ Try a Trial	2012-10-31
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	For Injection	103132		⤷ Try a Trial	2012-10-31
Merck Sharp & Dohme Corp.	INTRON A	interferon alfa-2b	Injection	103132		⤷ Try a Trial	2012-10-31
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

Make Better Decisions: Try a trial or see plans & pricing

Drugs may be covered by multiple patents or regulatory protections. All trademarks and applicant names are the property of their respective owners or licensors. Although great care is taken in the proper and correct provision of this service, thinkBiotech LLC does not accept any responsibility for possible consequences of errors or omissions in the provided data. The data presented herein is for information purposes only. There is no warranty that the data contained herein is error free. thinkBiotech performs no independent verification of facts as provided by public sources nor are attempts made to provide legal or investing advice. Any reliance on data provided herein is done solely at the discretion of the user. Users of this service are advised to seek professional advice and independent confirmation before considering acting on any of the provided information. thinkBiotech LLC reserves the right to amend, extend or withdraw any part or all of the offered service without notice.