Claims for Patent: 4,544,637

Summary for Patent: 4,544,637

Title:	Culture media from clarified diary whey lactose permeates
Abstract:	A method for separating bacteriological growth inhibitors from sweet whey ultrafiltrate to form a mother liquor which is useful as a bacteriological culture media and a precipitate which is useful as a food grade additive to cause clouding, stabilization, emulsification, and thickening of food, pharmaceutical, and cosmetic compositions.
Inventor(s):	Keggins; Kathleen M. (Glen Burnie, MD), Davis; Ann C. (College Park, MD), Sybert; Edward M. (Ellicott City, MD), Mays; Thomas D. (Burtonsville, MD), Milch; Robert A. (Baltimore, MD)
Assignee:	IGI Biotechnology, Inc. (Columbia, MD)
Application Number:	06/418,067
Patent Claims:	1. A dry powder microbiological culture medium which can be reconstituted with water and autoclaved to form a clear, pH neutral culture medium capable of supporting the growth of microorganisms under suitable growth conditions, said culture medium being prepared by a process consisting essentially of: (a) raising the pH of a dairy whey lactose permeate having a pH below about 7 to a selected pH between pH 8 and pH 10 at which essentially all of the dissolved solids which would become insoluble when the permeate is autoclaved for 10-20 minutes at 121 degrees C. and 15 psi precipitate as microcrystalline solids; (b) separating the supernatant from the resulting precipitate to form a microcrystalline solid phase consisting essentially of said previously dissolved solids; (c) removing components having a molecular weight above about 100 kdal from the supernatant to form a lactose-rich supernatant which is essentially free of said dissolved solids; and (d) drying the resultant supernatant to form said dry powder microbiological culture medium. 2. A dry powder microbiological culture medium according to claim 1 wherein said components have been removed in step (c) by ultrafiltration across a filter which retains components having a molecular weight above about 100 kdal. 3. A dry powder microbiological culture medium according to claim 2 wherein said filter retains components having a molecular weight above about 20 kdal. 4. A dry powder microbiological culture medium according to claim 1 further containing an added source of nontoxic assimilable carbon atoms. 5. A dry powder microbiological culture medium according to claim 4 wherein said source is glucose. 6. A dry powder microbiological culture medium according to claim 1 further containing an added source of nontoxic assimilable nitrogen atoms. 7. A dry powder microbiological culture medium according to claim 6 wherein said source is a yeast extract, hydrolyzed casein, or mixtures thereof. 8. A dry powder microbiological culture medium according to claim 1 further containing an added gelling agent in an amount capable of forming a gel when said dry powder is admixed with water. 9. A dry powder microbiological culture medium according to claim 8 further containing hydrolyzed casein, yeast extract, cysteine HCl, and glucose added in amounts effective to produce a microbiological culture medium suitable for the cultivation of both aerobic and anaerobic bacteria. 10. A dry powder microbiological culture medium according to claim 1 further containing hydrolyzed casein, yeast extract, and glucose added in amounts effective to produce a general purpose microbiological growth medium. 11. A dry powder microbiological culture medium according to claim 1 further containing hydrolyzed casein, yeast extract, glucose, and a colorimetric oxidation-reduction indicator added in amounts effective to produce a culture medium suitable for the cultivation of anaerobic bacteria. 12. A sterile microbiological culture medium consisting essentially of an aqueous solution of the dry powder culture medium according to claim 1. 13. A sterile microbiological culture medium according to claim 12 which has been sterilized by autoclaving. 14. A sterile microbiological culture medium according to claim 12 having a solids content of about 3.5% (wt/vol). 15. A sterile microbiological culture medium according to claim 12 having a total glucose content of about 0.5% and further containing additivies of about 0.5% hydrolyzed casein and about 0.05% yeast extract to produce a general purpose microbiological growth medium. 16. A sterile microbiological culture medium according to claim 12 having a total glucose content of about 0.4% and further containing additives of a nontoxic gelling agent in an amount effective to reduce oxygen diffusion, about 0.5% hydrolyzed casein, and about 0.05% yeast extract to produce a microbiological culture medium suitable for the cultivation of both aerobic and anaerobic bacteria. 17. A sterile microbiological culture medium according to claim 12 having a total glucose content of about 0.5% and further containing additives of about 0.5% hydrolyzed casein, about 1% yeast extract, about 0.2% cysteine HCl, about 0.5% hemin, about 0.1% vitamin K.sub.3, and an effective amount of a colorimetric oxidation-reduction indicator and having a pH of about 7.8 and an oxidation-reduction potential of -150 mV or less to produce a culture medium suitable for the cultivation of anaerobic bacteria.

Details for Patent 4,544,637

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Recordati Rare Diseases, Inc.	PANHEMATIN	hemin for injection	For Injection	101246	07/20/1983		2040-01-28