Claims for Patent: 4,152,868

Summary for Patent: 4,152,868

Title:	Method of producing mushroom spawn
Abstract:	An improved method of producing mushroom spawn without the necessity of employing a heated pressurized autoclave to sterilize the growth chamber and the mushroom mycelium growth medium contained therein. The elimination of the autoclave not only eliminates an expensive piece of equipment, but also saves substantial time, as well as the possibility of serious injuries that have occurred in the past in using heated autoclaves.
Inventor(s):	Lincoln; Richard G. (Long Beach, CA)
Assignee:
Application Number:	05/880,770
Patent Claims:	1. An improved method of growing mushroom spawn in a plurality of growth chambers, with each of said chambers having a removable cover that is vented to the ambient atmosphere through a filter, said method including the steps of: a. providing a clean enclosure substantially free of micro organisms; b. providing a source of a gaseous fumigant; c. subjecting a quantity of a mushroom mycelium growth nutrient to said fumigant to sterilize said nutrient; d. providing a quantity of sterile water in said clean enclosure; e. transporting said sterile nutrient to said clean enclosure and mixing the same with a quantity of sterile water; f. heating the mixture of sterile water and sterile nutrient in said clean room to cook the nutrient; g. sequentially sterilizing said growth chambers and covers and disposing said growth chambers and covers in said clean room; h. partially filling said growth chambers in said clean room with said heated nutrient after the latter has cooled to ambient temperature; i. inoculating said nutrient in said clean room in said growth chambers with mushroom mycelium of a desired species; j. placing said covers in sealed positions on said growth chambers after said inoculation; k. transporting said growth chambers with covers thereon to an area that is maintained at a temperature and environmental condition favorable to optimum growth of said mycelium, with said vented openings allowing escape of gases from said growth chambers, but said filters preventing the entry of micro organisms from the ambient atmosphere into the interior of said growth chambers; and l. allowing said mycelium to grow in said nutrient in said growth chambers until the spawn has grown through said nutrient and occupies a substantial part thereof. 2. An improved method of growing mushroom spawn as defined in claim 1 in which said nutrient is grain. 3. The method as defined in claim 1 in which the sterilizing of said growth chambers is by a gaseous fumigant.

Details for Patent 4,152,868

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	ZOSTAVAX	zoster vaccine live	For Injection	125123	05/25/2006		2039-09-23