Claims for Patent: 4,075,322

Summary for Patent: 4,075,322

Title:	Enzyme composition
Abstract:	An enzyme of hydrolytic action, according to the invention, is a neutral protease having the molecular weight of about 37,000, the isoelectric point at pH 8.8, the optimum activity at 40.degree. - 45.degree. C, the stability range in aqueous solutions at pH 6.8 - 8.0, quickly inactivating in said solutions at temperatures above 50.degree. C. The enzymatic preparation containing the enzyme of hydrolytic action in an amount of 50 - 500 PU/g and the metabolites of the strains Streptomyces hygroscopicus. A method for preparing the hydrolytic enzyme, in which the strain Streptomyces hygroscopicus is cultivated on media containing assimilable sources of carbon and nitrogen, inorganic mineral salts, in deep aerobic conditions, with accumulation of the enzyme of hydrolytic action and the metabolites of the said strains in the culture fluid, followed by subsequent isolation of the enzyme. A medicinal preparation for treating respiratory diseases, purulent wounds and thermal burns, for treating and preventing empyema of the pleura, containing the enzyme of hydrolytic action in the quantity of 1 - 50 PU/mg, inactive protein and mineral salts.
Inventor(s):	Bashkovich; Alexandr Pavlovich (Leningrad, SU), Zimnukhova; Evgenia Semenovna (Leningrad, SU), Prokopovich; Agnessa Vladimirovna (Leningrad, SU), Polatovskaya; Olga Grigorievna (Leningrad, SU), Pronina; Maria Ivanovna (Leningrad, SU), Kulbakh; Valter Osvaldovich (Leningrad, SU), Rivkina-Pevtsova; Khasya Ionovna (Leningrad, SU), Grinberg; Grigory Efimovich (Leningrad, SU)
Assignee:
Application Number:	05/593,536
Patent Claims:	1. A composition having hydrolytic, proteolytic and thrombolytic properties consisting essentially of an effective amount of hygrolytin and a pharmaceutically acceptable carrier, said hygrolytin being the product of the process which comprises cultivating, under deep aerobic conditions, Streptomyces hygroscopicus, strain 1913 (All-Union Research Institute of Antibiotics, Moscow, Culture Collection, Deposit No. 997), using an aqueous nutrient medium containing assimilable sources of carbon, nitrogen and inorganic substances, and separating hygrolytin formed during the culture. 2. The composition of claim 1, wherein the concentration of the hygrolytin is from 1 to 50 PU/mg. 3. The composition of claim 1, wherein the active principle is used in combination with a pharmaceutical solvent selected from the group consisting of sterile water for injections, a sodium chloride isotonic solution, a sodium chloride hypertonic solution, and a 0.25 to 2% solution of novocain. 4. The composition of claim 3, wherein the concentration of the active principle is in the range of 5 to 50 PU/ml. 5. The composition of claim 1, wherein the active principle is used in combination with a powdered filler for topical application, the filler being starch. 6. The composition of claim 5, wherein the concentration of the active principle is up to 500 PU per gram of filler.

Details for Patent 4,075,322

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Merck Sharp & Dohme Corp.	ZOSTAVAX	zoster vaccine live	For Injection	125123	05/25/2006		1995-02-21