Claims for Patent: 10,487,368

Summary for Patent: 10,487,368

Title:	Stabilization of rubisco activase for enhanced photosynthesis and crop yields
Abstract:	A modified rubisco activase, wherein the modified rubisco activase has a melting temperature greater than that of wild type rubisco activase. Further aspects of the disclosure relate to an isolated polynucleotide encoding a modified rubisco activase and a recombinant expression system comprising the isolated polynucleotide. Still further aspects of the disclosure relate to a plant cell transfected with the recombinant expression system. Certain aspects of the disclosure relate to a genetically modified plant expressing the isolated polynucleotide encoding a modified rubisco activase.
Inventor(s):	Pokkuluri; Phani R. (Westmont, IL), Schiffer; Marianne (Downers Grove, IL), Joachimiak; Andrej (Bolingbrook, IL), Wilton; Rosemarie (Elmhurst, IL)
Assignee:	UChicago Argonne, LLC (Chicago, IL)
Application Number:	15/690,247
Patent Claims:	1. A modified rubisco activase comprising a point mutation at amino acid positions 135 or 163 based on the sequence according to SEQ ID NO: 31, wherein the modified rubisco activase has a melting temperature greater than about 30.4.degree. C. 2. The modified rubisco activase of claim 1, wherein the point mutation at amino acid position 135 results in a substitution of R for the naturally occurring amino acid at this position, wherein the point mutation at amino acid position 163 results in a substitution of I for the naturally occurring amino acid at this position. 3. The modified rubisco activase of claim 1, wherein the modified rubisco activase further comprises a point mutation at amino acid position 262 based on the sequence according to SEQ ID NO: 31. 4. The modified rubisco activase of claim 1, wherein the modified rubisco activase is soy .beta. rubisco activase. 5. The modified rubisco activase of claim 2, wherein the modified rubisco activase comprises the point mutations at amino acid positions 135, 163, and further comprises a point mutation at amino acid position 262 according to SEQ ID NO: 31, wherein the point mutation at amino acid position 262 results in a substitution of I for the naturally occurring amino acid at this position. 6. The modified rubisco activase of claim 2, wherein the modified rubisco activase comprises point mutations at positions 135 and 163 based on the sequence according to SEQ ID NO: 31. 7. The modified rubisco activase of claim 1, wherein the modified rubisco activase is Miscanthus .beta. rubisco activase according to SEQ ID NO: 2, and wherein the amino acid positions 135 and 162 based on SEQ ID NO: 31 respectively correspond to amino acid positions 133 and 161 in SEQ ID NO: 2. 8. The modified rubisco activase of claim 7, wherein the modified rubisco activase has a melting temperature greater than about 39.8.degree. C. 9. The modified rubisco activase of claim 7, wherein the point mutation at amino acid position 133 results in a substitution of R for the naturally occurring amino acid at this position, wherein the point mutation at amino acid position 161 results in a substitution of I for the naturally occurring amino acid at this position. 10. The modified rubisco activase of claim 7, wherein the modified rubisco activase comprises point mutations at amino acid positions 133 and 161, and further comprises a point mutation at position 260 in SEQ ID NO: 2 that corresponds to position 262 according to SEQ ID NO: 31. 11. The modified rubisco activase of claim 1, wherein the modified rubisco activase has a melting temperature greater than about 34.5.degree. C. 12. The modified rubisco activase of claim 1, wherein the modified rubisco activase has a melting temperature greater than about 34.9.degree. C. 13. The modified rubisco activase of claim 1, wherein the modified rubisco activase has a melting temperature greater than about 38.8.degree. C. 14. The modified rubisco activase of claim 1, wherein the modified rubisco activase has a melting temperature greater than about 42.8.degree. C. 15. The modified rubisco activase of claim 1, wherein the modified rubisco activase has a melting temperature greater than about 44.0.degree. C. 16. The modified rubisco activase of claim 1, wherein the modified rubisco activase has a melting temperature greater than about 44.4.degree. C. 17. The modified rubisco activase of claim 1, wherein the modified rubisco activase has a melting temperature greater than about 46.0.degree. C. 18. An isolated polynucleotide encoding the modified rubisco activase of claim 1. 19. A recombinant expression system comprising the isolated polynucleotide of claim 18. 20. A plant cell transfected with the recombinant expression system of claim 19. 21. A genetically modified plant expressing the isolated polynucleotide of claim 18. 22. The genetically modified plant of claim 21, wherein the genetically modified plant is a genetically modified Arabidospis. 23. The genetically modified plant of claim 21, wherein the genetically modified plant is a genetically modified soybean plant. 24. The genetically modified plant of claim 21, wherein the genetically modified plant is a genetically modified Miscanthus giganteus.

Details for Patent 10,487,368

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Genentech, Inc.	ACTIVASE	alteplase	For Injection	103172	11/13/1987		2036-09-30
Genentech, Inc.	CATHFLO ACTIVASE	alteplase	For Injection	103172	09/04/2001		2036-09-30