Last Updated: August 15, 2026

Details for Patent: 5,723,147


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Summary for Patent: 5,723,147
Title:Multivesicular liposomes having a biologically active substance encapsulated therein in the presence of a hydrochloride
Abstract:Disclosed are multivesicular liposomes containing biologically active substances, the multivesicular liposomes having defined size distribution, adjustable average size, adjustable internal chamber size and number, and a modulated rate of the biologically active substance in contrast to the previous art. The process comprises dissolving a lipid component in volatile organic solvents, adding an immiscible aqueous component containing at least one biologically active substance to be encapsulated, and adding to either or both the organic solvents and the lipid component, a hydrochloride effective to control the release rate of the biologically active substance from the multivesicular liposome, making a water-in-oil emulsion from the two components, immersing the emulsion into a second aqueous component, dividing the emulsion into small solvent spherules which contain even smaller aqueous chambers, and then removing the solvents to give an aqueous suspension of multivesicular liposomes encapsulating biologically active substances.
Inventor(s):Sinil Kim, Stephen B. Howell
Assignee: Pacira Pharmaceuticals Inc
Application Number:US08/472,126
Patent Claim Types:
see list of patent claims
Use; Composition; Dosage form;
Patent landscape, scope, and claims:

US Patent 5,723,147 covered a multivesicular liposome platform in which hydrohalide concentration was used to modulate drug release. Its broadest claims reached compositions containing a biologically active substance, amphipathic lipid, neutral lipid, and 10-500 mM hydrochloric acid or selected hydrochloride salts. The patent also claimed therapeutic methods and narrower formulations for cytarabine, morphine sulfate, hydromorphone, amikacin, methotrexate, and iohexol.

The patent issued on March 3, 1998, and its 20-year statutory term expired in 2016, subject to any recorded patent-term adjustment. It therefore presents no current U.S. blocking right. Its historical importance is greater in the DepoFoam and DepoCyt patent families, where later or separate patents could have provided product-specific protection.

US Drug Patent 5,723,147: Claim Scope, Expiration, and Multivesicular Liposome Patent Landscape

What does US Patent 5,723,147 protect?

US 5,723,147 protects a release-controlled multivesicular liposome, or MVL, that contains a hydrohalide at a concentration of approximately 10 mM to 500 mM. The hydrohalide must be present in an amount sufficient to modulate release of the encapsulated active ingredient.

The core claim requires four technical elements:

Required element Claim requirement
Liposome architecture Multivesicular liposome with multiple non-concentric chambers
Active ingredient Broad list of therapeutic, biological, agricultural, diagnostic, and radioactive agents
Lipid system Amphipathic lipid plus neutral lipid lacking a hydrophilic head group
Release modifier Hydrochloric acid or specified hydrochloride salt at 10-500 mM

The patent is therefore a platform patent rather than a conventional compound patent. It does not claim cytarabine, morphine, amikacin, or another active ingredient in isolation. Infringement requires practice of the specified MVL architecture and hydrohalide-controlled formulation conditions.

The claims are composition, method-of-treatment, and formulation-specific claims. They do not require a particular injection route, dose, particle size, lipid identity, encapsulation efficiency, or release profile unless those limitations are introduced through a dependent claim.

How broad is claim 1 of US 5,723,147?

Claim 1 is the principal composition claim and has four substantial limitations.

First, the carrier must be a multivesicular liposome with multiple non-concentric chambers and membranes distributed in a matrix. This excludes ordinary unilamellar liposomes, simple multilamellar liposomes, polymeric microspheres, solid lipid particles, and non-liposomal depot systems.

Second, the formulation must contain an amphipathic lipid and a neutral lipid lacking a hydrophilic head group. The claim is directed to a two-component lipid system, not merely any lipid nanoparticle. The neutral lipid limitation is important because many modern lipid nanoparticles use ionizable lipids, cholesterol, phospholipids, and polyethylene glycol lipids without necessarily satisfying the claimed structural description.

Third, the formulation must contain one of the recited hydrohalides:

  • Hydrochloric acid;
  • Arginine hydrochloride;
  • Histidine hydrochloride;
  • Lysine hydrochloride; or
  • Pyridine hydrochloride.

Fourth, the hydrohalide concentration must fall within approximately 10 mM to 500 mM and must be sufficient to modulate release. The concentration range alone is not the entire limitation. The formulation must also have the claimed release-modulating function.

The active-ingredient list is unusually broad. It includes small molecules, nucleic acids, proteins, peptides, vaccines, hormones, radionuclides, pesticides, and herbicides. Claims 4 and 5 expressly extend the platform to pesticide and herbicide formulations, while claims 7 and 8 identify protein and hormone subcategories.

What formulations are protected by US 5,723,147?

The patent covers formulations in which hydrohalide concentration changes the release behavior of the encapsulated material. The specification and dependent claims distinguish between conditions that increase and decrease release rates.

The most commercially relevant narrow formulations are:

Claim Active ingredient Hydrohalide condition Claimed effect
9 Cytarabine, morphine sulfate, hydromorphone, amikacin, methotrexate, or iohexol 10-500 mM selected hydrohalide Release modulation
11 Amikacin, morphine sulfate, or cytarabine As required by claim 9 Composition limitation
13 Cytarabine 70-500 mM hydrochloric acid Decreases release rate
14 Cytarabine 10-70 mM hydrochloric acid Increases release rate
15 Morphine sulfate 25-200 mM hydrochloric acid Decreases release rate

Claims 13 and 14 create a concentration-dependent split for cytarabine. A cytarabine MVL containing 10 mM to less than approximately 70 mM hydrochloric acid is directed toward increased release, while a formulation containing approximately 70 mM to 500 mM is directed toward decreased release.

The boundary at approximately 70 mM creates potential claim-construction and testing issues. A product near that boundary would require precise measurement of the encapsulation-phase concentration and analysis of whether the formulation produces the claimed release effect.

How do claims 6 and 12 cover treatment methods?

Claim 6 covers administering a biologically active substance encapsulated in an MVL when encapsulation occurred in the presence of one of the specified hydrohalides at 10-500 mM. Claim 12 narrows the active ingredient to cytarabine, morphine sulfate, hydromorphone, amikacin, or methotrexate.

The method claims require more than administration of the active ingredient. They require:

  1. Encapsulation in a multivesicular liposome;
  2. Use of a specified hydrohalide during encapsulation;
  3. A hydrohalide concentration within the claimed range; and
  4. Release modulation, with claim 12 further requiring a therapeutically effective release rate.

Claims 3 and 10 add a therapeutic-effect limitation requiring a rate sufficient to ameliorate disease in a living mammal. These limitations may narrow infringement but can create evidentiary disputes because they depend on the clinical and pharmacokinetic behavior of the formulation.

Claims 6 and 12 also contain drafting inconsistencies. Claim 6 refers to "a hydrochloride" while listing hydrochloric acid and hydrochloride salts. Claim 12 uses similar terminology. The terminology would likely be interpreted in the context of the specification and claim set rather than according to strict chemical nomenclature alone.

When did US 5,723,147 lose exclusivity?

US 5,723,147 lost its U.S. patent exclusivity in 2016. The patent issued March 3, 1998, under the post-1995 patent-term regime, which generally provides a term of 20 years from the earliest effective U.S. nonprovisional filing date under 35 U.S.C. § 154.

Milestone Date
U.S. patent application 1996
Patent issuance March 3, 1998
Standard statutory term 20 years from applicable nonprovisional filing date
Expected expiration 2016
Current status Expired

The patent is not a live U.S. exclusion right. Patent-term adjustment, if any, would be reflected in the USPTO record. No patent-term extension under the pharmaceutical regulatory extension statute should be assumed for this platform patent without a specific extension record.

The expiration of US 5,723,147 does not eliminate later patents covering particular MVL products, manufacturing processes, lipid combinations, formulations, or drug-specific delivery systems.

What is the Orange Book status of US 5,723,147?

US 5,723,147 is a platform patent and is not inherently an Orange Book-listed patent. The FDA Orange Book lists patents associated with approved drug products when the NDA holder submits them and the listing meets the applicable statutory and regulatory categories. Platform patents may be listed for a drug product, but a patent number does not become an Orange Book patent merely because it concerns drug delivery.

For a product such as liposomal cytarabine, Orange Book analysis must distinguish:

  • The NDA’s listed drug substance patents;
  • Drug product or formulation patents;
  • Method-of-use patents;
  • Manufacturing patents, which generally are not Orange Book-listed in the same manner;
  • Expired patents that remain visible historically but do not block approval or launch.

The relevant FDA regulatory pathway for a generic equivalent would depend on whether the reference product is treated as an eligible reference listed drug and whether the proposed product can demonstrate pharmaceutical equivalence, bioequivalence, and equivalent clinical performance. Complex liposomal products can require a more extensive analytical and clinical package than conventional immediate-release small-molecule products. FDA, “Approved Drug Products with Therapeutic Equivalence Evaluations.” [2]

Which products are most closely associated with this patent?

The most relevant commercial connection is the DepoFoam MVL technology and DepoCyt, a liposomal formulation of cytarabine. DepoCyt used multivesicular liposomes to provide sustained release of cytarabine after intrathecal administration. Its formulation and regulatory history are separate from the legal scope of US 5,723,147, but cytarabine is expressly identified in claims 9, 11, 12, 13, and 14.

Product or technology Relationship to US 5,723,147
DepoFoam Platform technology associated with multivesicular liposomal delivery
DepoCyt Liposomal cytarabine product; directly relevant to the cytarabine claim set
Liposomal morphine systems Relevant to claims 9, 11, 12, and 15
Liposomal amikacin systems Relevant to claims 9, 11, and 12
Liposomal methotrexate systems Relevant to claims 9 and 12
Iohexol MVL systems Relevant to claim 9, but not the treatment method claim in claim 12

The FDA approved DepoCyt in 1999 for intrathecal treatment of lymphomatous meningitis. The label described a sustained-release liposomal cytarabine product administered by the intrathecal route. [3] DepoCyt was later withdrawn from the U.S. market for commercial reasons, not because US 5,723,147 remained enforceable.

How does US 5,723,147 compare with other multivesicular liposome patents?

The patent landscape is divided into five groups.

Core MVL architecture patents

These patents claim the physical structure and preparation of multivesicular liposomes. They are potentially broader than drug-specific formulation patents because they may cover many active ingredients and release profiles.

Hydrohalide release-modulation patents

US 5,723,147 occupies this category. Its distinctive limitation is the use of hydrochloric acid or selected hydrochloride salts during encapsulation to alter release.

Drug-specific MVL patents

Separate patents may claim cytarabine, morphine sulfate, hydromorphone, amikacin, or other actives in an MVL. These patents can create barriers even after expiration of a broader platform patent.

Manufacturing patents

MVL manufacturing may involve double-emulsion or multiple-emulsion processes, lipid deposition, solvent removal, aqueous-phase selection, and control of internal chamber composition. A later entrant could avoid US 5,723,147 yet infringe a process patent covering a particular manufacturing sequence.

Commercial product patents

Commercial products may have patents directed to dosage, route of administration, treatment schedules, stability, vial composition, or a particular sustained-release profile. These patents are usually more relevant to launch timing than an expired platform patent.

Known related patent numbers in the MVL and DepoFoam field include US 5,766,627 and US 5,932,241, which should be reviewed separately for filing dates, continuations, terminal disclaimers, and expiration status. Their relevance cannot be inferred solely from similar titles or shared inventorship. The USPTO patent file and complete family records control. [1]

What patent litigation affects US 5,723,147?

US 5,723,147 has no current enforcement value because its statutory term expired in 2016. Historical litigation or licensing involving DepoFoam, DepoCyt, or related MVL patents may still matter for:

  • Interpretation of MVL structural terms;
  • Ownership and chain-of-title analysis;
  • Inventorship;
  • Settlement scope;
  • License restrictions;
  • Damages periods before expiration;
  • Continuation and divisional patent rights.

A Paragraph IV challenge to US 5,723,147 itself is no longer commercially meaningful. Paragraph IV certifications are directed to listed patents associated with an approved drug and are used to challenge patents before expiration. An expired patent cannot ordinarily support a forward-looking launch injunction, although it can remain relevant to historical regulatory records and litigation databases.

No current generic-entry risk should be assigned to this patent alone. Risk must be assessed against unexpired patents covering the reference product, formulation, method of use, or manufacturing process.

How strong is the patent estate for a competing MVL product?

The expired patent is technically broad but legally weak as a current barrier. Its strongest historical features were:

Strength factor Assessment
Broad active-ingredient definition Strong historical breadth
Defined MVL architecture Meaningful structural limitation
Hydrohalide concentration range Clear numerical limitation
Functional release requirement Supports technical distinction but creates proof issues
Specific cytarabine claims Stronger product relevance
Expiration Eliminates current exclusionary force
Platform coverage Does not by itself block later patented products
Manufacturing coverage Not established by the claims provided

A competing product would face the greatest current risk from later, unexpired patents covering the specific lipid composition, particle architecture, manufacturing process, dosage regimen, or active ingredient. It would not face a live infringement risk from US 5,723,147 after expiration.

What generic launch scenarios exist for liposomal cytarabine and similar products?

A generic or follow-on entrant could pursue several pathways:

  1. A conventional abbreviated application, if FDA determines that the proposed product can demonstrate the required equivalence through standard analytical and bioequivalence methods.
  2. A suitability or alternative pathway, where the product differs in dosage form or formulation and FDA requires additional evidence.
  3. A 505(b)(2) application, particularly if the proposed product relies on published literature, a listed drug, or changes to the delivery system.
  4. A full NDA pathway if the product cannot rely sufficiently on the reference product or if clinical bridging is substantial.

For complex liposomal products, the main barriers are usually formulation comparability, particle-size distribution, internal aqueous-phase characterization, release testing, sterility, stability, and clinical pharmacokinetics. Patent expiry reduces the legal barrier but does not remove the FDA development burden.

Does US 5,723,147 create biosimilar risk?

No conventional biosimilar pathway is implicated by this patent. The claims cover liposomal formulations of proteins, peptides, vaccines, cytokines, and other biologically active substances, but the patent is not a biologic composition-of-matter patent.

A protein product using an MVL delivery system could face:

  • Biologic reference-product exclusivity;
  • Device or delivery-system patents;
  • Formulation patents;
  • Manufacturing patents;
  • Clinical and analytical comparability requirements.

Those issues arise independently of the expired patent. The patent’s inclusion of interleukin-2, granulocyte colony-stimulating factor, hepatitis B antigen, GM-CSF, IGF-1, and alpha-interferon does not create current biosimilar exclusivity.

Key Takeaways

  • US 5,723,147 covers hydrohalide-modulated multivesicular liposomes.
  • The core elements are MVL architecture, amphipathic lipid, neutral lipid without a hydrophilic head group, and 10-500 mM hydrochloric acid or specified hydrochloride salts.
  • Claims 9-15 focus on cytarabine, morphine sulfate, hydromorphone, amikacin, methotrexate, and iohexol.
  • Claims 13 and 14 divide cytarabine formulations at approximately 70 mM hydrochloric acid based on release direction.
  • The patent issued March 3, 1998, and expired in 2016 under the standard post-1995 patent term.
  • It is not a current U.S. launch blocker.
  • Orange Book and Paragraph IV risk must be evaluated against later patents associated with the specific approved product.
  • The principal continuing risks are later formulation, process, dosage, method-of-use, and product-specific patents.
  • DepoCyt and DepoFoam are the most commercially relevant technologies for understanding the patent family and historical licensing environment.
  • Patent expiry does not remove FDA complexity for generic or follow-on liposomal products.

FAQs About US Patent 5,723,147

Is US 5,723,147 still enforceable?

No. Its standard 20-year patent term ended in 2016, making it unavailable as a current U.S. exclusion right.

Does the patent cover ordinary liposomes?

No. The claims require a multivesicular liposome with multiple non-concentric chambers and membranes distributed in a matrix.

Does the patent cover all liposomal cytarabine products?

No. A cytarabine product must also satisfy the MVL structure, lipid requirements, hydrohalide conditions, and release-modulation limitations.

Can a company launch a liposomal morphine product without reviewing this patent?

Yes as to this expired patent, but later patents covering morphine formulation, manufacturing, dosage, administration, or release characteristics must be assessed.

Was US 5,723,147 a composition-of-matter patent for cytarabine?

No. It was a delivery-platform and formulation patent. Cytarabine was one of several active ingredients recited in narrower claims.

References

  1. United States Patent and Trademark Office. (1998). Multivesicular liposomes with modulated release rates, U.S. Patent No. 5,723,147.
  2. U.S. Food and Drug Administration. (2024). Approved drug products with therapeutic equivalence evaluations.
  3. U.S. Food and Drug Administration. (1999). DepoCyt (liposomal cytarabine) prescribing information.
  4. United States Code. (2024). 35 U.S.C. § 154: Contents and term of patent; provisional rights.
  5. United States Patent and Trademark Office. (2024). Patent Center and patent term information records.

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Drugs Protected by US Patent 5,723,147

Applicant Tradename Generic Name Dosage NDA Approval Date TE Type RLD RS Patent No. Patent Expiration Product Substance Delist Req. Patented / Exclusive Use Submissiondate
>Applicant >Tradename >Generic Name >Dosage >NDA >Approval Date >TE >Type >RLD >RS >Patent No. >Patent Expiration >Product >Substance >Delist Req. >Patented / Exclusive Use >Submissiondate

Foreign Priority and PCT Information for Patent: 5,723,147

Foriegn Application Priority Data
Foreign Country Foreign Patent Number Foreign Patent Date
United Kingdom8704171Feb 23, 1987

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