Claims for Patent: 6,225,284

Summary for Patent: 6,225,284

Title:	Somatostatin peptides
Abstract:	Somatostain analogues comprising the amino acid sequence of the formula (I): -(D/L)Trp-Lys-X1-X2, wherein X1 is a substituted Thr, Ser, Tyr, Glu or Cys residue and X2 is an α-amino acid having an aromatic residue on the Cα side chain, or an amino acid unit selected from Dab, Dpr, Dpm, His (Bzl)HyPro, thienyl-Ala, cyclohexyl-Ala and t.-butyl-Ala, the residue Lys of said sequence corresponding to the residue Lys9 of the native somatostatin-14, in free form, in salt form or complexed with a detectable element have interesting pharmacological properties.
Inventor(s):	Rainer Albert, Wilfried Bauer, Christian Bruns, Nagarajan Chandramouli, Ian Lewis, Gisbert Weckbecker
Assignee:	Recordati SA
Application Number:	US08/981,426
Patent Claims:	1. A somatostatin analogue which is a hexapeptide unit numbered from 1 to 6, the residues at positions 3 through 6 of said hexapeptide unit having the sequence of formula I -(D/L)Trp-Lys-X1-X2- (1) wherein X1 is a radical of formula (a) or (b) wherein R1 is optionally substituted phenyl, R2 is —Z1CH2—R1, —CH2—CO—O—CH2—R1, wherein Z1 is O or S, and X2 is an α-amino acid having an aromatic residue on the Cα side chain, or an amino acid unit selected from Dab, Dpr, Dpm, His, (Bzl)HyPro, thienyl-Ala, cyclohexyl-Ala and t.-butyl-Ala, the residue Lys of said sequence corresponding to the residue Lys9 of the native somatostatin-14, or a chelate thereof, in free form or in salt or complex form. 2. A somatostatin analogue according to claim 1 in which the hexapeptide unit is cyclic with a direct peptide linkage between the α-carbonyl group of the residue at position 6 and the α-amino group of the residue at position 1, or a chelate thereof, in free form or in salt or complex form. 3. A somatostatin analogue according to claim 1 which is a compound of formula (II) wherein X1 and X2 are as in claim 1, A is a divalent residue selected from the group consisting of Pro, wherein R3 is NR8R9-C2-6alkylene, guanidino-C2-6alkylene or C2-6alkylene-COOH, R3a is H, C1-4alkyl or has independently one of the significances given for R3, R3b is H or C1-4alkyl, Ra is OH or NR5R6, Rb is —(CH2)1-3— or —CH(CH3)—, R4 is H or CH3, R4a is optionally ring-substituted benzyl, each of R5 and R6 independently is H, C1-4alkyl, ω-amino-C1-4alkylene, ω-hydroxy-C1-4alkylene or acyl, R7 is a direct bond or C1-6alkylene, each of R8 and R9 independently is H, C1-4alkyl, ω-hydroxy-C2-4alkylene, acyl or CH2OH—(CHOH)c—CH2— wherein c is 0, 1, 2, 3 or 4, or R8 and R9 form together with the nitrogen atom to which they are attached a heterocyclic group which may comprise a further heteroatom, and R11 is optionally ring-substituted benzyl, —(CH2)1-3—OH, CH3—CH(OH)— or —(CH2)1-5—NR5R6, and ZZa is a natural or unnatural α-amino acid unit, or a chelate thereof, in free form or in salt or complex form. 4. A somatostatin analogue according to claim 1 wherein A comprises an amino group bearing a chelating group, in free form, in salt form or complexed with a detectable element. 5. A process for the production of a somatostatin analogue according to claim 1 comprising a) removing at least one protecting group which is present in a somatostatin analogue comprising a residue of formula I, the somatostatin analogue being in protected form, or b) linking together by an amide bond two peptide units, each of them containing at least one amino acid in protected or unprotected form, wherein the amide bond is in such a way that the desired amino acid sequence is obtained and, where required, effecting process step a), or c) removing a functional group of an unprotected or a protected somatostatin peptide or converting it into another functional group so that another unprotected or protected peptide is obtained and in the latter case stage a) of the process is effected, or d) to produce a chelated somatostatin analogue linking together a chelating agent and a non-chelated somatostatin analogue in protected or unprotected form and comprising a free amino group in such a way that the chelating group is fixed on the desired amino group of the somatostatin analogue, and stage a) is then optionally effected and recovering the somatostatin analogue thus obtained in free form, in salt form or optionally complexed with a detectable element. 6. A pharmaceutical composition comprising a pharmaceutical acceptable carrier or diluent and a therapeutically effective amount of a somatastatin analogue according to claim 1 or a chelate thereof, or a pharmaceutically acceptable salt or a complex with a detectable element. 7. A method of treating disorders with an aetiology comprising or associated with excess GH-secretion, gastro-intestinal disorders, malignant cell proliferative diseases, angiogenesis, or of preventing or combating graft vessel diseases, restenosis and vascular occlusion following vascular injury, which method comprises administering to a subject in need of such treatment a therapeutically effective amount of a somatostatin analogue of claim 1 or a chelate thereof, or a pharmaceutically acceptable salt or a complex with a detectable element.