Claims for Patent: 11,053,502

Summary for Patent: 11,053,502

Title:	Therapeutic inhibition of lactate dehydrogenase and agents therefor
Abstract:	This invention relates to compounds, compositions, and methods useful for reducing lactate dehydrogenase target RNA and protein levels via use of dsRNAs, e.g., Dicer substrate siRNA (DsiRNA) agents.
Inventor(s):	Bob D. Brown, Henryk T. Dudek, Cheng Lai
Assignee:	Novo Nordisk Health Care AG
Application Number:	US17/110,121
Patent Claims:	1. A double stranded ribonucleic acid (dsRNA) agent for inhibiting expression of lactic acid dehydrogenase A (LDHA), wherein the dsRNA comprises a sense strand and an antisense strand forming a double stranded region, wherein the antisense strand comprises at least 19 consecutive nucleotides differing by 3 or fewer nucleotides from the nucleotide sequence 5′-UCAGAUAAAAAGGACAACAUGC-3′ (SEQ ID NO: 7193), wherein the antisense strand is 19 to 35 nucleotides in length. 2. The dsRNA agent of claim 1, wherein the antisense strand comprises at least 19 consecutive nucleotides differing by 2 or fewer nucleotides from the nucleotide sequence 5′-UCAGAUAAAAAGGACAACAUGC-3′ (SEQ ID NO: 7193). 3. The dsRNA agent of claim 1, wherein the antisense strand comprises at least 19 consecutive nucleotides differing by 1 or fewer nucleotides from the nucleotide sequence 5′-UCAGAUAAAAAGGACAACAUGC-3′ (SEQ ID NO: 7193). 4. The dsRNA agent of claim 1, wherein the antisense strand comprises at least 19 consecutive nucleotides from the nucleotide sequence 5′-UCAGAUAAAAAGGACAACAUGC-3′ (SEQ ID NO: 7193). 5. The dsRNA agent of claim 1, wherein all of the nucleotides of the double-stranded region are modified nucleotides. 6. The dsRNA agent of claim 5, wherein the modified nucleotides are selected from the group consisting of 2′-O-methyl-modified nucleotides and 2′-fluoro-modified nucleotides. 7. The dsRNA agent of claim 1, wherein all of the nucleotides of the sense and antisense strands are modified nucleotides. 8. The dsRNA agent of claim 1, wherein the dsRNA agent is attached to one or more N-acetylgalactosamine (GalNAc) moieties. 9. The dsRNA agent of claim 1, wherein the dsRNA agent comprises a phosphate backbone modification selected from the group consisting of a methylphosphonate, a phosphorothioate and a phosphotriester. 10. The dsRNA agent of claim 1, wherein the dsRNA agent comprises a phosphorothioate linkage. 11. The dsRNA agent of claim 1, wherein the sense strand and the antisense strand comprise a phosphorothioate linkage. 12. The dsRNA agent of claim 1, wherein the antisense strand is 19 to 23 nucleotides in length. 13. The dsRNA agent of claim 1, wherein the antisense strand is 22 nucleotides in length. 14. The dsRNA agent of claim 1, wherein the sense strand and the antisense strand are fully complementary. 15. The dsRNA agent of claim 1, wherein the antisense strand comprises a 3′ overhang of 2 nucleotides. 16. The dsRNA agent of claim 1, wherein the dsRNA agent reduces LDHA mRNA levels by at least 70% in a cell when provided to the cell at a concentration of at least 1 nM. 17. A pharmaceutical composition comprising the dsRNA agent of claim 1 and a pharmaceutically acceptable carrier. 18. A double stranded ribonucleic acid (dsRNA) agent for inhibiting expression of lactic acid dehydrogenase A (LDHA), wherein the dsRNA comprises a sense strand and an antisense strand forming a double stranded region, wherein the antisense strand comprises at least 19 consecutive nucleotides differing by 3 or fewer nucleotides from the nucleotide sequence 5′-UCAGAUAAAAAGGACAACAUGC-3′ (SEQ ID NO: 7193); wherein the antisense strand is 19 to 35 nucleotides in length; wherein all of the nucleotides of the double-stranded region are modified nucleotides, and wherein the modified nucleotides are selected from the group consisting of 2′-O-methyl-modified nucleotides and 2′-fluoro-modified nucleotides; and wherein the dsRNA is attached to one or more N-acetylgalactosamine (GalNAc) moieties. 19. The dsRNA agent of claim 18, wherein the antisense strand comprises at least 19 consecutive nucleotides from the nucleotide sequence 5′-UCAGAUAAAAAGGACAACAUGC-3′ (SEQ ID NO: 7193). 20. The dsRNA agent of claim 19, wherein the sense strand and the antisense strand comprise a phosphorothioate linkage. 21. The dsRNA agent of claim 20, wherein the antisense strand is 22 nucleotides in length; and wherein the sense strand and the antisense strand are fully complementary. 22. The dsRNA agent of claim 21, wherein the sense strand is between 32 and 80 nucleotides in length and comprises a tetraloop. 23. A pharmaceutical composition comprising the dsRNA agent of claim 20 and a pharmaceutically acceptable carrier. 24. A double stranded ribonucleic acid (dsRNA) agent for inhibiting expression of lactic acid dehydrogenase A (LDHA), wherein the dsRNA comprises a sense strand and an antisense strand forming a double stranded region, wherein the antisense strand comprises at least 15 consecutive nucleotides differing by 4 or fewer nucleotides from the nucleotide sequence 5′-UCAGAUAAAAAGGACAACAUGC-3′ (SEQ ID NO: 7193), wherein the antisense strand is 19 to 35 nucleotides in length. 25. The dsRNA agent of claim 24, wherein all of the nucleotides of the double stranded region are modified nucleotides, and wherein the modified nucleotides are selected from the group consisting of 2′-O-methyl-modified nucleotides and 2′-fluoro-modified nucleotides; and wherein the dsRNA is attached to one or more N-acetylgalactosamine (GalNAc) moieties. 26. The dsRNA agent of claim 25, wherein the antisense strand is 19 to 30 nucleotides in length, and wherein the sense strand is between 32 and 80 nucleotides in length and comprises a tetraloop. 27. A pharmaceutical composition comprising the dsRNA agent of claim 25 and a pharmaceutically acceptable carrier. 28. A mammalian cell comprising the dsRNA agent of claim 1. 29. A mammalian cell comprising the dsRNA agent of claim 20. 30. A mammalian cell comprising the dsRNA agent of claim 25.