Claims for Patent: 9,267,148

Summary for Patent: 9,267,148

Title:	Methods for the biosynthesis of taurine or hypotaurine in cells
Abstract:	The present invention describes an approach to increase taurine or hypotaurine production in prokaryotes or eukaryotes. More particularly, the invention relates to genetic transformation of organisms with genes that encode proteins that catalyze the conversion of cysteine to taurine, methionine to taurine, cysteamine to taurine, or alanine to taurine. The invention describes methods for the use of polynucleotides that encode functional cysteine dioxygenase (CDO), cysteine dioxygenase (CDO) and sulfinoalanine decarboxylase (SAD) or glutamate decarboxylase (GAD), cysteamine dioxygenase (ADO), taurine-pyruvate aminotransferase (TPAT), TPAT and sulfoacetaldehyde acetyltransferase (SA), taurine dioxygenase (TDO) or the small (ssTDeHase) and large subunits of taurine dehydrogenase (lsTDeHase) polypeptides in plants to increase taurine, hypotaurine or taurine precursor production. The preferred embodiment of the invention is in plants but other organisms may be used. Increased taurine production in plants will enhance plant growth and development, yield, or tolerance to biotic and/or abiotic stresses and could be used as nutraceutical, pharmaceutical, or therapeutic compounds or as a supplement in animal feed.
Inventor(s):	Turano; Frank J. (Baltimore, MD), Turano; Kathleen A. (Baltimore, MD), Carlson; Peter S. (Baltimore, MD), Kinnersley; Alan M. (Baltimore, MD)
Assignee:	PLANT SENSORY SYSTEMS, LLC (Baltimore, MD)
Application Number:	13/505,415
Patent Claims:	1. A plant cell comprising a first exogenous DNA which comprises two separate expression cassettes, wherein a first expression cassette comprises a first promoter operably linked to a first polynucleotide and a second expression cassette comprises a second promoter operably linked to a second polynucleotide, wherein: the first polynucleotide encodes cysteine dioxygenase (CDO); and the second polynucleotide encodes sulfinoalanine decarboxylase (SAD). 2. A plant cell comprising a first exogenous DNA which comprises a single expression cassette, wherein the single expression cassette comprises a promoter operably linked to a polynucleotide which encodes (i) CDO and SAD; (ii) fused CDO-SAD; (iii) fused CDO-linker-SAD; (iv) fused SAD-CDO; or (v) fused SAD-linker-CDO. 3. A plant cell comprising a first exogenous DNA which comprises a promoter operably linked to a polynucleotide, wherein the polynucleotide encodes CDO. 4. The plant cell of claim 1 wherein: the CDO comprises the amino acid sequence SEQ ID NO:3 or the amino acid sequence SEQ ID NO:4; and the SAD comprises the amino acid sequence SEQ ID NO:7 or the amino acid sequence SEQ ID NO:8. 5. The plant cell of claim 3 wherein: the CDO comprises the amino acid sequence SEQ ID NO:3 or the amino acid sequence SEQ ID NO:4. 6. The plant cell of claim 1 wherein the first polynucleotide comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2. 7. The plant cell of claim 1 wherein the second polynucleotide comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:5 and SEQ ID NO:6. 8. The plant cell of claim 2 wherein the polynucleotide comprises a nucleotide sequence encoding CDO selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2 and a nucleotide sequence encoding SAD selected from the group consisting of SEQ ID NO:5 and SEQ ID NO:6. 9. The plant cell of claim 3 wherein the polynucleotide comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2. 10. The plant cell of claim 1 wherein at least one of the first and second promoters is a constitutive promoter. 11. The plant cell of claim 2 wherein the promoter is a constitutive promoter. 12. The plant cell of claim 1 wherein at least one of the first and second promoters is a non-constitutive promoter. 13. The plant cell of claim 2 wherein the promoter is a non-constitutive promoter. 14. The plant cell of claim 12 wherein the non-constitutive promoter is selected from the group consisting of a tissue-preferred promoter, a tissue-specific promoter, a cell type-specific promoter, an inducible promoter, a plant glutamate decarboxylase (GAD) promoter, plant sulphate transporter (SULTR) promoter, and a plant glutamate receptor (GLR) promoter. 15. The plant cell of claim 13 wherein the non-constitutive promoter is selected from the group consisting of a tissue-preferred promoter, a tissue-specific promoter, a cell type-specific promoter, an inducible promoter, a plant glutamate decarboxylase (GAD) promoter, plant sulphate transporter (SULTR) promoter, and a plant glutamate receptor (GLR) promoter. 16. The plant cell of claim 1 wherein one or both of the first and second polynucleotides comprises a subcellular location sequence. 17. The plant cell of claim 2 wherein the polynucleotide comprises a subcellular location sequence. 18. The plant cell of claim 16 wherein the subcellular location sequence targets a subcellular location selected from the group consisting of an apoplast, a vacuole, a plastid, a chloroplast, a proplastid, an etioplast, a chromoplast, a mitochondrion, a peroxisome, a glyoxysome, a nucleus, a lysosome, an endomembrane system, an endoplasmic reticulum, a vesicle, and a Golgi apparatus. 19. A plant storage organ comprising the plant cell of claim 1. 20. The plant storage organ of claim 19 which a seed, a tuber, a fruit, or a root. 21. A transgenic seed having in its genome (i) exogenous DNA encoding CDO or (ii) exogenous DNA encoding CDO and SAD, wherein enzymatic activity of the protein increases production of taurine or hypotaurine in a transgenic plant grown from the transgenic seed. 22. The transgenic seed of claim 21 wherein: the CDO comprises the amino acid sequence SEQ ID NO:3 or SEQ ID NO:4; and the SAD comprises the amino acid sequence SEQ ID NO:7 or SEQ ID NO:8. 23. The transgenic seed of claim 21 wherein (i) the exogenous DNA comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2 or (ii) the exogenous DNA comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2 and a nucleotide sequence selected from the group consisting of SEQ ID NO:5 and SEQ ID NO:6. 24. A transgenic plant grown from the transgenic seed of claim 21. 25. The transgenic plant of claim 24 which is selected from the group consisting of acacia, alfalfa, algae, aneth, apple, apricot, artichoke, arugula, asparagus, avocado, banana, barley, beans, beech, beet, Bermuda grass, blackberry, blueberry, Blue grass, broccoli, brussels sprouts, cabbage, camelina, canola, cantaloupe, carrot, cassava, cauliflower, celery, cherry, chicory, cilantro, citrus, clementines, coffee, corn, cotton, cucumber, duckweed, Douglas fir, eggplant, endive, escarole, eucalyptus, fennel, fescue, figs, forest trees, garlic, gourd, grape, grapefruit, honey dew, jicama, kiwifruit, lettuce, leeks, lemon, lime, Loblolly pine, maize, mango, melon, mushroom, nectarine, nut, oat, okra, onion, orange, ornamental plants, papaya, parsley, pea, peach, peanut, pear, pepper, persimmon, pine, pineapple, plantain, plum, pomegranate, poplar, potato, pumpkin, quince, radiata pine, radicchio, radish, rapeseed, raspberry, rice, rye, rye grass, seaweed, scallion, sorghum, Southern pine, soybean, spinach, squash, strawberry, sugarbeet, sugarcane, sunflower, sweet potato, sweetgum, switchgrass, tangerine, tea, tobacco, tomato, turf, turnip, a vine, watermelon, wheat, yam, and zucchini. 26. A method of producing a crop of transgenic plants, comprising multiplying or breeding plant material to obtain a crop of transgenic plants from the transgenic seed of claim 21. 27. A method of producing taurine or hypotaurine, comprising growing the plant cell of claim 1 under conditions which permit expression of the first and second polynucleotides, thereby producing taurine or hypotaurine. 28. A method of producing taurine or hypotaurine, comprising growing the plant cell of claim 2 under conditions which permit expression of the polynucleotide, thereby producing taurine or hypotaurine. 29. The method of claim 27 further comprising introducing the exogenous DNA into the plant cell. 30. A transgenic plant having in its genome (i) exogenous DNA encoding CDO or (ii) exogenous DNA encoding CDO and SAD, wherein the plant has increased growth, yield, or biomass, altered development, or increased tolerance to biotic (pests, pathogens, bacteria, microbes, viruses, viroids, microorganisms, invertebrates, insects, nematodes, or vertebrates) or abiotic (changes in osmotic conditions, oxidative damage, drought, salt, cold, freezing, heat, UV light or light intensity) stress. 31. A transgenic plant having in its genome (i) exogenous DNA encoding CDO or (ii) exogenous DNA encoding CDO and SAD, wherein the plant has increased water-use-efficiency or nitrogen-use-efficiency. 32. A transgenic plant having in its genome (i) exogenous DNA encoding CDO or (ii) exogenous DNA encoding CDO and SAD, wherein the seeds have increased oil, starch or protein. 33. A method of altering a property of a transgenic plant, comprising contacting a transgenic plant having in its genome (i) exogenous DNA encoding CDO or (ii) exogenous DNA encoding CDO and SAD, with an agent which increases sulfur or nitrogen concentration in cells of the transgenic plant, wherein the property is selected from the group consisting of increased plant growth, altered plant development, increased tolerance to biotic stress, increased tolerance to abiotic stress, increased yield, and increased biomass. 34. The method of claim 33 wherein the agent increases sulfur concentration and the agent is selected from the group consisting of fertilizer, sulfur, sulfite, sulfide, sulfate, taurine, hypotaurine, homotaurine, homocysteine, cystathionine cysteate, 2-sulfacetaldehyde, N-acetyl thiazolidine 4 carboxylic acid (ATCA), glutathione, and bile. 35. The method of claim 33 wherein the agent increases nitrogen concentration and the agent is selected from the group consisting of an amino acid and a polyamine. 36. The method of claim 35 wherein the agent is an amino acid and the amino acid is not naturally present in a protein. 37. The method of claim 36 wherein the amino acid is GABA, citrulline, or ornithine. 38. The method of claim 35 wherein the agent is an amino acid and the amino acid is selected from the group consisting of cysteine, methionine, glutamate, glutamine, serine, alanine, and glycine. 39. A pharmaceutical composition comprising an extract of the transgenic plant of claim 24, wherein the extract comprises taurine, hypotaurine, homotaurine, taurine derivatives, taurine conjugates, or taurine polymers. 40. A nutritional supplement comprising an extract of the transgenic plant of claim 24. 41. An animal feed supplement comprising the plant storage organ of claim 19. 42. An animal feed supplement comprising the transgenic seed of claim 21. 43. An animal feed supplement comprising the transgenic plant of claim 24. 44. The plant cell of claim 17 wherein the subcellular location sequence targets a subcellular location selected from the group consisting of an apoplast, a vacuole, a plastid, a chloroplast, a proplastid, an etioplast, a chromoplast, a mitochondrion, a peroxisome, a glyoxysome, a nucleus, a lysosome, an endomembrane system, an endoplasmic reticulum, a vesicle, and a Golgi apparatus.

Details for Patent 9,267,148

Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Aimmune Therapeutics, Inc.	PALFORZIA	peanut (arachis hypogaea) allergen powder-dnfp	Powder	125696	January 31, 2020		2030-10-29